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A. A. Plotnikov

Bio: A. A. Plotnikov is an academic researcher from Southern Federal University. The author has contributed to research in topics: Oxidative stress & Superoxide dismutase. The author has an hindex of 3, co-authored 9 publications receiving 51 citations.

Papers
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Journal ArticleDOI
TL;DR: It is hypothesized that the protective effect of SkQ1 under hyperoxia-induced oxidative stress could be realized via direct antioxidant activity and through stimulation of the signaling defense system Keap1/Nrf2/ARE.
Abstract: The administration of SkQ1 to rats at the dose of 50 nmol/kg for five days significantly increased the mRNA levels of transcription factor Nrf2 and of Nrf2-controlled genes encoding antioxidant enzymes SOD1, SOD2, CAT, and GPx4, whereas changes in the level of mRNA of SOD3 in the cerebral cortex of the rat brain were not significant. This was accompanied by activation of antioxidant enzymes (SOD, CAT, GPx, and GST) and increase in reduced glutathione concentration. Under oxidative stress induced by hyperoxia (0.5 MPa for 90 min), the mRNA level of transcription factor Nrf2 decreased, whereas changes in the transcriptional activity of Nrf2-induced genes (SOD1-3, CAT, GPx4) encoding antioxidant enzymes in the cortex of the rat brain hemispheres were insignificant. Under conditions of hyperoxia, lipid peroxidation intensity was increased, CAT was inhibited, and GST activity was moderately increased, whereas SOD and GPx activities in the rat brain cerebral cortex remained at the stationary level. Pretreatment with SkQ1 before the exposure to hyperbaric oxygenation led to an increase in mRNA level of transcription factor Nrf2 and of Nrf2-induced genes (SOD1-2, CAT, and GPx4) encoding antioxidant enzymes, whereas SOD3 expression in the cerebral cortex of the rat brain under oxidative stress was not changed. Concurrently, we observed an increase in activities of these antioxidant enzymes (SOD, CAT, GPx, and GST) and in level of reduced glutathione. We hypothesize that the protective effect of SkQ1 under hyperoxia-induced oxidative stress could be realized via direct antioxidant activity and through stimulation of the signaling defense system Keap1/Nrf2/ARE.

26 citations

Journal ArticleDOI
TL;DR: Circulating NOx level and a combination of IL-6 and leptin permitted the strongest discrimination of patients with PTOA from controls and was correlated with leptin levels in plasma and SF and with the synovial IL-18 level.
Abstract: The identification of biomarkers of post-traumatic osteoarthritis (PTOA) progression is of clinical importance. The aims of this study were: (1) to assess the abilities of various soluble proinflammatory mediators in plasma to distinguish patients with knee PTOA from controls; (2) to determine the correlations between the mediators in plasma and those mediators in synovial fluid (SF); and (3) to explore the associations of the mediators with radiographic PTOA severity. The concentrations of IL-1β, IL-6, IL-18, TNFα, and leptin were measured using ELISA. Nitric oxide was determined as nitrite/nitrate (NO x ) using the Griess reaction. We included 171 subjects (134 PTOA patients and 37 controls) and excluded patients with rheumatoid arthritis or gout. The ROC curve of plasma NO x had the highest AUC, a specificity of 100%, and a sensitivity of 84.4%. The combination of IL-6 and leptin proved to be the most discriminatory, with an AUC value of 0.933, a specificity of 96.7%, and a sensitivity of 85.7%. The levels of NO x , IL-6, IL-18, and leptin in plasma were significantly correlated with their levels in SF. Leptin levels in both plasma (p = 0.036) and SF (p = 0.041) and the synovial IL-18 level (p = 0.045) were correlated with the Kellgren–Lawrence (KL) grade. Early-stage PTOA (KL 1–2) was associated with a high concentration of IL-1β in plasma before and after (OR 6.235, 95% CI 1.362 to 28.552, p = 0.018) adjusting for age, gender, and BMI. Circulating NO x level and a combination of IL-6 and leptin permitted the strongest discrimination of patients with PTOA from controls. PTOA severity was correlated with leptin levels in plasma and SF and with the synovial IL-18 level. Early PTOA was associated with the circulating level of IL-1β. III (case–control study).

22 citations

Journal ArticleDOI
TL;DR: In this paper, a comparative analysis of physio-biochemical indices and transcriptional activity of oxidative stress genes in barley (Hordeum vulgare L.) seedlings after 7-days exposure to bulk- and nano-ZnO (300 and 2000 ǫ/L) was carried out.

20 citations

Journal ArticleDOI
TL;DR: It is hypothesized that protective effect of SkQ1 in hyperoxia-induced oxidative stress can be realized via a direct antioxidant property and the stimulation of the Keap1/Nrf2 redox-sensitive signaling system.
Abstract: The study demonstrated that oxidative stress induced by hyperoxia (0.5 MPa for 90 min) resulted in reduction of mRNA levels of transcription factor Nrf2 and Nrf2-induced genes encoding antioxidant enzymes (SOD1, CAT, GPx4) in peripheral blood leukocytes of rats. The changes in gene expression profiles under hyperoxia were accompanied by disbalance of activity of antioxidant enzymes in the leukocytes, namely activation of superoxide dismutase and inhibition of catalase, glutathione peroxidase, and glutathione-S-transferase. Pretreatment of rats with SkQ1 (50 nmol/kg for five days) significantly increased mRNA levels of transcription factor Nrf2 and Nrf2-induced genes encoding antioxidant enzymes SOD2 and GPx4 and normalized the transcriptional activity of the SOD1 and CAT genes in the leukocytes in hyperoxia-induced oxidative stress. At the same time, the activity of catalase and glutathione peroxidase was increased, and the activity of superoxide dismutase and glutathione-S-transferase returned to the control level. It is hypothesized that protective effect of SkQ1 in hyperoxia-induced oxidative stress can be realized via a direct antioxidant property and the stimulation of the Keap1/Nrf2 redox-sensitive signaling system.

9 citations

Journal Article
TL;DR: Redox-balance disturbance in the mononuclear cells leaded to oxidative stress development, which promoted lymphocytes apoptosis, which led to oxidativestress development in patients with knee osteoarthritis.
Abstract: The features of regulation of free radical oxidation in the blood and the synovial fluid have been studied in patients with knee osteoarthritis. The data were obtained from 46 patients with primary knee osteoarthritis (II-III Kellgren-Lawrence grade, aged 63.26 ± 9.18) and 27 healthy controls. It was defined that the blood plasma was characterized by increased superoxide-eliminating activity and a tendency to the increasing of nitrite/nitrate concentration (nitrosative stress biomarkers). The erythrocytes were measured to have the increased concentration of malondialdehyde (secondary product of lipid peroxidation), activation of superoxide dismutase, gIutathione-S-transferase and reduced glutathione peroxidase activity and glutathione content. The peripheral blood mononuclear fraction of patients with osteoarthritis was characterized by significant activation of superoxide dismutase and glutathione-S- transferase, moderate catalase activation, non-significant glutathione peroxidase activity alterations and increased activity of xanthine oxidoreductase and myeloperoxidase. Finally redox-balance disturbance in the mononuclear cells leaded to oxidative stress development, which promoted lymphocytes apoptosis.

5 citations


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Journal Article
TL;DR: This study demonstrates the existence of 3 linked mutations in the 5′-flanking region of the eNOS gene (T−786→C, A−922→G, and T−1468→A) and shows that the most predictive independent risk factor for coronary spasm was the mutant allele.
Abstract: Background—Coronary spasm plays an important role in the pathogenesis of ischemic heart diseases in general. However, the precise mechanism(s) responsible for coronary spasm remains to be elucidated, and we examined the molecular genetics of coronary spasm. Methods and Results—We searched for the possible mutations in the endothelial nitric oxide synthase (eNOS) gene in patients with coronary spasm. In this study, we demonstrate the existence of 3 linked mutations in the 5′-flanking region of the eNOS gene (T−786→C, A−922→G, and T−1468→A). The incidence of the mutations was significantly greater in patients with coronary spasm than in the control group (P<0.0001). Multiple logistic regression analysis with forward stepwise selection using the environmental risk factors and the eNOS gene variant revealed that the most predictive independent risk factor for coronary spasm was the mutant allele (P<0.0001). As assessed by luciferase reporter gene assays, the T−786→C mutation resulted in a significant reductio...

269 citations

Journal ArticleDOI
TL;DR: In this paper, the authors analyzed individual cytokines involved in the immune processes with an emphasis on their function in osteoarthritis and found that the concentration of both proinflammatory and anti-inflammatory cytokines were altered depending on the OA stage and activity.
Abstract: Osteoarthritis is a common cause of disability worldwide. Although commonly referred to as a disease of the joint cartilage, osteoarthritis affects all joint tissues equally. The pathogenesis of this degenerative process is not completely understood; however, a low-grade inflammation leading to an imbalance between anabolic and katabolic processes is a well-established factor. The complex network of cytokines regulating these processes and cell communication has a central role in the development and progression of osteoarthritis. Concentrations of both proinflammatory and anti-inflammatory cytokines were found to be altered depending on the osteoarthritis stage and activity. In this review, we analyzed individual cytokines involved in the immune processes with an emphasis on their function in osteoarthritis.

99 citations

Journal ArticleDOI
TL;DR: The structure of the polysaccharide and its phosphorylated derivative was determined by IR and NMR, which indicated that the chemical modification was successful and could significantly increase the content of SOD and CAT in serum, liver and spleen tissue of mice.

90 citations

Journal ArticleDOI
TL;DR: Polysaccharide, as one of the effective components of traditional Chinese medicine, has the activities of anti‐oxidation, anti‐virus, improving immunity and so on, and through the endogenous antioxidant stress Nrf2/ARE pathway, it can significantly increase the antioxidant capacity and reduce oxidative stress injury.
Abstract: Polysaccharide, as one of the effective components of traditional Chinese medicine, has the activities of anti-oxidation, anti-virus, improving immunity and so on. Chinese herbal polysaccharides have a wide range of sources. It is found that polysaccharides in many plants and microorganisms have significant antioxidant effects, mainly through the endogenous antioxidant stress Nrf2/ARE pathway to regulate the expression of downstream antioxidant enzymes. These antioxidant enzymes can further block the free radical chain reaction, thus reducing the generation of free radicals. Secondly, by inhibiting the expression of iNOS mRNA and reducing NO production, it can significantly increase the antioxidant capacity and reduce oxidative stress injury. Polysaccharides are rich in natural resources, their multi-channel, multi-target, multi-effect, and other characteristics have made great progress in antioxidants.

73 citations

Journal ArticleDOI
TL;DR: Sulforaphane‐treated BTBR and C57 mice had reduced self‐grooming/marble burying behavior, and increased social interaction in three chambered sociability test as compared to untreated BTBR mice, reason that activation of Nrf2 by sul foraphane corrected Th17 immune dysfunction and oxidant‐antioxidant imbalance in periphery and brain inBTBR mice.

61 citations