A. Benabdeljlil

Bio: A. Benabdeljlil is an academic researcher. The author has contributed to research in topics: Colipase & Lipase. The author has an hindex of 1, co-authored 1 publications receiving 35 citations.

Ovine pancreatic lipase : purification and some properties.

Abstract: Summary Lipase has been isolated from sheep pancreas. The lipoprotein complex formed in pancreas homogenates by the enzyme and endogenous lipids is split by treatment with acetone. Lipase is further purified by ion-exchange chromatography and gel filtration. The molecular weight and the amino-acid composition of ovine lipase are very similar to that of the porcine and bovine enzymes. As previously found in bovine lipase, no carbohydrate is covalently bound to the polypeptide chain which has a N-terminal residue of lysine. The study of the catalytic properties of ovine pancreatic lipase indicates that the enzyme is fully activated by colipase from various species in the presence of conjugated bile salt micellar solutions.

Digestion, absorption and transport of lipids in ruminant animals.

Abstract: Publisher Summary This chapter discusses the digestion, absorption, and transport of lipids in ruminant animals. In the simple-stomached animal, the processes of digestion and absorption of dietary fats begin essentially when they reach the small intestine. In the ruminant animal, the situation is very different and the events that occur within the complex polygastric arrangement of the alimentary tract, principally pregastric microbial fermentation of cellulose and other plant polymers not normally hydrolyzed by digestive enzymes, have a profound effect on the chemical and physical nature of the lipids subsequently presented to the small intestine for digestion. Two major processes occur within the rumen, which have an important bearing on the composition and distribution of the lipid components of the digesta and their subsequent metabolism within the intestine. These processes, which are intrinsically bound together, are lipolysis of the dietary lipids and hydrogenation of their unsaturated fatty-acid constituents. These make an immense contribution directly to the lipid metabolism of the host animal after absorption from the rumen and indirectly through their involvement in bacterial and protozoal lipid synthesis.

Human pancreatic lipase: A glycoprotein

Abstract: Human lipase has been purified from pancreatic juice. The protein has a molecular weight of 48 000 and an N-terminal residue of lysine. It has been characterized as a glycoprotein containing 4.7 mol of glucosamine, 2.8 mol of mannose, 2.9 mol of fucose, 3.0 mol. of galactose and 1.1 mol of glucose per mol of protein. Two isolipases have been separated by polyacrylamide gel electrophoresis. Their isoelectric points are 5.80 and 5.85, respectively and both are glycoproteins. Immunological cross reactions have been obtained between human lipase and other mammalian lipases (porcine, bovine, ovine, canine and rat).

Lipases from Mammals and Fishes

Abstract: Lipases are a broad family of enzymes that catalyze the hydrolysis of ester bonds in substrates such as triglycerides, phospholipids, cholesteryl esters, and vitamin esters. Lipases are receiving increasing interest due to their effects on the quality of food products (e.g., the quality of post-harvest seafoods), and their actual and potential applications in modified foods and industrial processes. Lipases that catalyze specific reactions and that are active at particular conditions of pH and temperature to suit the requirements of industrial processes are of particular interest. This review focuses on lipases that display predominantly triacylglycerol hydrolase activity. Section 1 presents an overview of lipases from terrestrial organisms and the lipase gene family members. Due to their unique physiology, diet, and habitat, fish lipases may demonstrate novel activities that have potential applications for bioindustrial catalysis. Section 2 discusses lipases from fish and compares them with lipases from ...

Pancreatic and microbial lipases: A comparison of the interaction of pancreatic colipase with lipases of various origins

Abstract: Conjugated bile salts inhibit the hydrolysis of triglycerides (TG) by the lipases fromRhizopus arrhizus andGeotrichum candidum. This occurs for detergent concentrations similar to those which suppress the action of mammalian pancreatic lipases upon the same substrates. However, in opposition with what is observed with the latter enzymes, the activity is not restored by the addition of pancreatic colipase. Both pancreatic andR. arrhizus lipases are inactivated at tributyrin/water interface, but only the first enzyme is protected against this surface denaturation by the pancreatic cofactor. These observations suggest that colipases synthesized in mammalian pancreas display specific interaction towards the lipases made by the same organ.

Digestion, absorption and transport of lipids in ruminant animals

Abstract: Publisher Summary This chapter discusses the digestion, absorption, and transport of lipids in ruminant animals. In the simple-stomached animal, the processes of digestion and absorption of dietary fats begin essentially when they reach the small intestine. In the ruminant animal, the situation is very different and the events that occur within the complex polygastric arrangement of the alimentary tract, principally pregastric microbial fermentation of cellulose and other plant polymers not normally hydrolyzed by digestive enzymes, have a profound effect on the chemical and physical nature of the lipids subsequently presented to the small intestine for digestion. Two major processes occur within the rumen, which have an important bearing on the composition and distribution of the lipid components of the digesta and their subsequent metabolism within the intestine. These processes, which are intrinsically bound together, are lipolysis of the dietary lipids and hydrogenation of their unsaturated fatty-acid constituents. These make an immense contribution directly to the lipid metabolism of the host animal after absorption from the rumen and indirectly through their involvement in bacterial and protozoal lipid synthesis.