A. N. Ivanova

Bio: A. N. Ivanova is an academic researcher from Siberian Federal University. The author has contributed to research in topics: Raw material & Food processing. The author has an hindex of 2, co-authored 10 publications receiving 5 citations.

Products of sea-buckthorn berries processing in parapharmaceutical production

Abstract: Labour activity of the person during scientific and technical progress is complicated with increasing ecology pollution caused by active use of various chemicals for agricultural purposes, industrial use of toxic metals and other substances, radioactive components of different origin. The purpose of our research is to find out new vegetative raw material being a source of food fibers (in the given concrete case) and possessing high food and biological value which could become a basis for creation new kinds of foodstuff for medical and prophylactic use in the diet of the population directly involved in harmful manufacture at the industrial enterprises and living in ecologically polluted areas. We offer to use the sea-buckthorn berries oil cake as such source of raw material - the dry waste product from sea-buckthorn oil production, which possesses high biological activity and meets all listed above requirements.

Phytohormone content in microstrobiles and androgenic callus of Siberian larch

Abstract: Androgenesis in vitro in plants is a phenomenon of developmental switching of male generative cells, microspores, from their normal gametophytic to sporophytic pathway. We obtained androgenic callus and embryoids (embryo-like structures derived from microspores) of the conifer plant, Siberian larch (Larix sibirica Ledeb.) in the in vitro culture. The immune-enzyme analysis of the hormonal balance of larch androgenic cultures showed a substantial increase in the content of all phytohormones, especially cytokinins and ABA, as compared with initial explants (microstrobiles). This was evidently related to active cell divisions and embryoid formation. A comparison of androgenic cultures derived from trees nonifested and infested with larch gall midges (Dasineura rozhkovi Mam. et Nik.) revealed a cytokinin content increase (by two times) and an ABA content decrease (by two times), which indicates more intense growth of cultures derived from healthy trees. Phytohormone content in the androgenic callus was compared with their accumulation in the embryos of larch seeds harvested from noninfested trees. We concluded that successful growth of androgenic cultures and embryoid formation demand an additional medium supplement with auxins (not more than 0.5 mg/l).

Research of the influence of vacuum packaging on the quality and safety of meat semi-finished products

Abstract: The paper investigates the effect of vacuum packaging on the quality and safety of semi-finished meat products. The microbiological and organoleptic characteristics of a small-sized semi-finished product from pork pulp (goulash, pieces weighing 20-30 g) were studied, part of which was packed in GN 1/4 gastronorm containers, 0.5 kg each, the other part was packed in a vacuum package of 0.5 kg vacuum packer HVC-260T/1A (DZ-260/PD) using three-layer PA/PE bags (polyamide with polyethylene) with dimensions of 250x350 mm and a thickness of 90 microns. All semi-finished products were stored under the same conditions, in a refrigerator at a temperature of 4 °C. Comparative microbiological analysis showed that the number of mesophilic aerobic and facultative anaerobic microorganisms in semi-finished pork meat products stored using traditional technologies exceeds the semi-finished products stored in vacuum packaging. In terms of sanitary and hygienic indicators, the test products meet the requirements of TR CU 021/2011 and SanPiN 2.3.2.1078-01 for 8 days of storage at a temperature of 4 ± 2 ° C. In the investigated semi-finished products revealed shifts in pH (5.12-6.75) during storage and subsequent regeneration. Vacuum packing contributes to the preservation of organoleptic characteristics of meat semi-finished products, in comparison with semi-finished products that were stored traditionally. Analysis of the totality of quality and safety indicators for meat semi-finished products stored in vacuum packaging allows predicting an increase in shelf life up to 8 days. The shelf life of the investigated meat semi-finished products stored in vacuum packaging is 5 times longer than the shelf life established by SanPiN 2.3.2.1324-03.

Androgenesis-inducing stress treatments change phytohormone levels in anthers of three legume species (Fabaceae).

Abstract: Legumes are recalcitrant to androgenesis and induction protocols were only recently developed for pea (Pisum sativum L.) and chickpea (Cicer arietinum L.), albeit with low regeneration frequencies. Androgenesis is thought to be mediated through abscisic acid (ABA) but other phytohormones, such as auxins, cytokinins, and gibberellins, have also been implicated. In view of improving induction protocols, the hormone content of pea, chickpea, and lentil anthers was measured after exposure to cold, centrifugation, electroporation, sonication, osmotic shock, or various combinations thereof using an analytical mass spectrometer. Indole-3-acetic acid (IAA) had a key function during the induction process. In pea, high concentrations of IAA-asparagine (IAA-Asp), a putative IAA metabolite, accumulated during the application of the different stresses. In chickpea, the IAA-Asp concentration increased 30-fold compared to pea but only during the osmotic shock treatment and likely as a result of the presence of exogenous IAA in the medium. In contrast, no treatment in lentil (Lens culinaris) invoked such an increase in IAA-Asp content. Of the various cytokinins monitored, only cis zeatin riboside increased after centrifugation and electroporation in pea and possibly chickpea. No bioactive gibberellins were detected in any species investigated, indicating that this hormone group is likely not linked to androgenesis in legumes. In contrast to the other stresses, osmotic shock treatment caused a reduction in the levels of all hormones analyzed, with the exception of IAA-Asp in chickpea. A short period of low hormone content might be a necessary transition phase for androgenesis induction of legumes. Key message Five androgenesis-inducing stress treatments changed content of ABA, auxin and cytokinin in anthers of three legumes. Osmotic shock treatment differed because it reduced hormone content to very low levels.

Somaclonal variation of haploid in vitro tissue culture obtained from Siberian larch ( Larix sibirica Ledeb.) megagametophytes for whole genome de novo sequencing

Abstract: The objective of this study was to obtain a genetically stable haploid in vitro-derived line from Siberian larch (Larix sibirica Ledeb.) using megagametophyte explants, which then could be used for different molecular genetic studies, including whole genome de novo sequencing. However, cytogenetic analysis and genotyping of 11 microsatellite loci showed high levels of genomic instability and a high frequency of mutation in the obtained megagametophyte-derived callus cultures. All cultures contained new mutations in one or more microsatellite loci.

Genome-wide analysis of the GRAS gene family exhibited expansion model and functional differentiation in sea buckthorn (Hippophae rhamnoides L.)

Abstract: Genome-wide identification of the sea buckthorn GRAS genes and their transcription profiles in different organizations. GRAS proteins comprise a large family of transcription factors that experienced extensive replication, and play important roles in many aspects of growth regulatory and environmental signals. Sea buckthorn is a unique and valuable plant which has tremendous value for medical researching, ecological protection, and providing daily necessities. We identified, characterized, and distinguished duplication models of GRAS transcription factors in sea buckthorn. A systematic whole-genome analysis was performed to identify and characterize the GRAS gene family of sea buckthorn, especially the comparative genome method was used to demonstrate its different duplication models. We identified 62 sea buckthorn GRAS (HrGRAS) genes and renamed based on their respective chromosome distribution. Fifty-nine HrGRASs were classified into nine subgroups and three HrGRASs did not belong to any of the subfamilies according to their phylogenetic features. HrGRAS genes tend to have a representative GRAS domain, few introns, and unevenly distributed on chromosomes. Segmental duplication was the main driver of the GRAS gene family expansion, followed by whole-genome duplication (WGD) and tandem duplication, according to the results of comparative genome analysis. GRAS genes involved in duplication experienced strong purifying selection pressure according to the Ka/Ks ratios. The expression profile derived from transcriptome data shows the expression patterns of HrGRAS gene in different tissues and the characterization of differentially expressed genes. This systematic analysis provided a foundation to understand the expansion and potential functions of GRAS genes with an aim of sea buckthorn crop improvement.

Valorization of Berries’ Agro-Industrial Waste in the Development of Biodegradable Pectin-Based Films for Fresh Salmon (Salmo salar) Shelf-Life Monitoring

Abstract: The healthy properties of berries are known; however, red fruits are very perishable, generating large losses in production and marketing. Nonetheless, these wastes can be revalued and used. The main objective of this study was the development of biodegradable pectin films with berry agro-industrial waste extracts to monitor salmon shelf-life. The obtained extracts from blueberries, blackberries, and raspberries wastes were evaluated in terms of flavonols, phenols and anthocyanins contents, and antioxidant capacity. Then, pectin films with the extracts of different berries were developed and characterized. The results showed that the blueberry extract film was thicker (0.248 mm), darker (L* = 61.42), and opaquer (17.71%), while the highest density (1.477 g/cm3) was shown by the raspberry films. The results also showed that blueberries were the best for further application due to their composition in bioactive compounds, antioxidant capacity, and color change at different pHs. The salmon samples wrapped in blueberry films showed lower values of pH and deterioration of fish during storage compared to the control and pectin samples. This study contributes to the valorization of berries agro-industrial waste by the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative.