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Adam J. Taylor

Bio: Adam J. Taylor is an academic researcher from National Physical Laboratory. The author has contributed to research in topics: Mass spectrometry & Mass spectrometry imaging. The author has an hindex of 8, co-authored 17 publications receiving 499 citations. Previous affiliations of Adam J. Taylor include University of Nottingham & University of Washington.

Papers
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TL;DR: In this paper, the authors applied a stringent decellularization process to demineralized bone matrix (DBM), prepared from bovine bone, and characterized the structure and composition of the resulting ECM materials and DBM itself.

228 citations

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TL;DR: Time of flight secondary ion mass spectroscopy (ToF-SIMS) is used to provide sensitive, molecular specific, localized analysis of detergent decellularized biologic scaffolds and demonstrates the importance of maintaining a balance between cell removal and detergent disruption of matrix architecture and matrix surface ligand landscape.

172 citations

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TL;DR: This study explored several aspects of regional analyte suppression in mass spectrometry imaging (MSI) of a heterogeneous sample, transverse cryosections of mouse brain and employed the concept of a tissue extinction coefficient (TEC) to assess suppression of an analyte on tissue relative to its intensity in an off tissue region.
Abstract: In this study we have explored several aspects of regional analyte suppression in mass spectrometry imaging (MSI) of a heterogeneous sample, transverse cryosections of mouse brain. Olanzapine was homogeneously coated across the section prior to desorption electrospray ionization (DESI) and matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging. We employed the concept of a tissue extinction coefficient (TEC) to assess suppression of an analyte on tissue relative to its intensity in an off tissue region. We expanded the use of TEC, by first segmenting anatomical regions using graph-cuts clustering and calculating a TEC for each cluster. The single ion image of the olanzapine [M + H]+ ion was seen to vary considerably across the image, with anatomical features such as the white matter and hippocampus visible. While trends in regional ion suppression were conserved across MSI modalities, significant changes in the magnitude of relative regional suppression effects between techniques we...

90 citations

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TL;DR: In this paper, the development of the coordination chemistry of dithiolene ligands is summarised, together with a consideration of the electronic structure of complexes of these ‘non-innocent’ ligands.

73 citations

Journal ArticleDOI
TL;DR: Investigation of physiological responses during delay of gratification in relation to emotionality, self-regulation, and adjustment problems points to children who are over- and under-regulated with implications for adjustment problems.

31 citations


Cited by
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09 Mar 2012
TL;DR: Artificial neural networks (ANNs) constitute a class of flexible nonlinear models designed to mimic biological neural systems as mentioned in this paper, and they have been widely used in computer vision applications.
Abstract: Artificial neural networks (ANNs) constitute a class of flexible nonlinear models designed to mimic biological neural systems. In this entry, we introduce ANN using familiar econometric terminology and provide an overview of ANN modeling approach and its implementation methods. † Correspondence: Chung-Ming Kuan, Institute of Economics, Academia Sinica, 128 Academia Road, Sec. 2, Taipei 115, Taiwan; ckuan@econ.sinica.edu.tw. †† I would like to express my sincere gratitude to the editor, Professor Steven Durlauf, for his patience and constructive comments on early drafts of this entry. I also thank Shih-Hsun Hsu and Yu-Lieh Huang for very helpful suggestions. The remaining errors are all mine.

2,069 citations

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TL;DR: An exploration of the structural, mechanical, biochemical and biological information present in native human tissue for bioengineering applications is focused on to provide inspiration for the design of future biomaterials.

754 citations

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TL;DR: The present manuscript represents a review of ECM hydrogels and attempts to identify structure-function relationships that influence the tissue remodeling outcomes and gaps in the understanding thereof.

499 citations

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TL;DR: Challenges and opportunities of ECM biomaterials are investigated for the design of organotypic models to study disease progression, for the ex vivo creation of engineered tissue and for the clinical translation of functional tissue reconstruction strategies in vivo.
Abstract: In tissue engineering and regenerative medicine, a biomaterial provides mechanical support and biochemical signals to encourage cell attachment and modulate cell behaviour. Nature’s template for a biomaterial is the extracellular matrix (ECM). The ECM contains intrinsic biochemical and mechanical cues that regulate cell phenotype and function in development, in homeostasis and in response to injury. The use of ECM-based materials in biomedical research has advanced from coating cell culture plates with purified ECM components to the design of ECM-mimicking biomaterials and the engineering of decellularized tissues aimed at recapitulating the dynamics, composition and structure of the ECM. In this Review, we highlight important matrix properties and functions in the context of tissue engineering and regenerative medicine, consider techniques such as proteomics for the investigation of matrix structure and composition and discuss different engineering strategies for the design of matrix-mimicking biomaterials. Tissue, whole organ and cell culture decellularization approaches are examined for their potential to preserve the tissue-specific biochemical composition and ultrastructure of the ECM and for the development of biomaterials that promote the formation of functional tissues in clinical applications. Finally, we investigate challenges and opportunities of ECM biomaterials for the design of organotypic models to study disease progression, for the ex vivo creation of engineered tissue and for the clinical translation of functional tissue reconstruction strategies in vivo. The extracellular matrix is nature’s template for an ideal biomaterial to guide tissue homeostasis and repair. In this Review, matrix-mimicking biomaterials and decellularized matrices are discussed for their potential to reconstruct and repair tissues in vitro and in vivo.

494 citations

Journal ArticleDOI
TL;DR: In this paper, the most common instances where non-innocent behaviour of redox-active ligands, either substrates or supporting components, is observed in a biochemical context are discussed.

453 citations