Afshin Tavakoli

Bio: Afshin Tavakoli is an academic researcher from University of Zanjan. The author has contributed to research in topics: Drought tolerance & Dracocephalum moldavica. The author has an hindex of 5, co-authored 9 publications receiving 81 citations.

Growth and Stomatal Responses of Bread Wheat Genotypes in Tolerance to Salt stress

Abstract: Plant growth is affected by the osmotic stress as well as toxicity of salt in leaves. In order to study of salt stress effects on stomatal conductance and growth rate and relationship between them as wells osmotic and Na-specific effects on these traits, four bread wheat genotypes differing in salt tolerance were selected. Salinity was applied when the leaf 4 was fully expanded. Sodium (Na) concentrations in flag leaf blade at 3 salinity levels (0, 100 and 200 mM NaCl) were measured. Salt-tolerant genotypes showed higher stomatal conductance and growth rate compared to salt-sensitive ones. After 10 and 20 days exposure to salt, stomatal conductance and relative growth rate were reduced, but the reduction was greater in sensitive genotypes. Growth rate was reduced severely in the first period (1-10 days) of salt commencements and it was due to osmotic effect of salt not Na toxicity. In the second period (11-20 days) after salt treatment growth reduced only when salt accumulated to toxic concentrations in the leaves. A positive relationship between stomatal conductance and relative growth rate showed that stomatal conductance can be a reliable indicator of growth rate, and finally can be considered as a sensitive indicator of the osmotic stress. It seems 20 days after salinity, the major effect of salt, especially at low to moderate salinity levels on growth properties was due to the osmotic effect of salt, not to Na-specific effects within the plant. Keywords—Osmotic stress, Relative growth rate, Stomatal conductance, Wheat.

Concentrations-dependent effect of exogenous abscisic acid on photosynthesis, growth and phenolic content of Dracocephalum moldavica L. under drought stress.

Abstract: The drought conditions and the application of ABA reduce the photosynthetic activity, and the processes related to the transpiration of Dracocephalum moldavica L. At the same time, the plant increases the production of phenolic compounds and essential oil as a response to stress conditions. In the semi-arid regions, drought stress is the most important environmental limitations for crop production. Abscisic acid (ABA) plays a crucial role in the reactions of plants towards environmental stress such as drought. Field experiments for two consecutive years in 2016 and 2017 were conducted to evaluate the effect of three watering regimes (well-watered, moderate and severe drought) and five exogenous ABA concentrations (0, 5, 10, 20 and 40 μM) on growth, photosynthesis, total phenolic and essential oil content of Dracocephalum moldavica L. Without ABA application, the highest photosynthetic rate (6.1 μmol CO2 m−2 s−1) was obtained under well-watered condition and, moderate and severe drought stress decreased photosynthesis rate by 26.39% and 34.43%, respectively. Some growth parameters such as stem height, leaf area, leaf dry weight and biological yield were also reduced by drought stress. ABA application showed a decreasing trend in photosynthesis rate and mentioned plant growth parameters under all moisture regimes. The highest seed yield (1243.56 kg ha−1) was obtained under well-watered condition without ABA application. Increasing ABA concentration decreased seed yield in all moisture regimes. The highest total phenolic content (8.9 mg g−1 FW) and essential oil yield (20.58 kg ha−1) were obtained from 20 and 5 μM ABA concentration, respectively, under moderate drought stress.

Analytical overview of DNA interaction with Morin and its metal complexes

Abstract: Flavonoids play an important role in pharmaceutical and biomedical sciences Various flavonoids, especially Morin, have potential to interact with DNA Their prospective clinical and pharmacological utilities have attracted researchers’ attention in last two decades Due to their interaction with DNA and their anticancer effects, a lot of investigations have been carried out all over the world In this paper, we will review the properties of Morin and its different metal complexes as well as their interactions with DNA that have been reported by several research groups Also, further details have been provided about binding mechanisms of these materials with DNA using analytical techniques such as UV–Visible and fluorescence spectroscopies, viscosity and voltammetry Structural analysis showed Morin binds to DNA through non-intercalation mode, but its metal complexes interact with DNA via intercalation mode and binding mode of Morin, and its metal complexes with DNA will be helpful in the determination of anticancer drugs binding mechanism to DNA Therefore, it will be useful in new drug designs

Study the effect of 24-epibrassinolide application on the Cu/Zn-SOD expression and tolerance to drought stress in common bean

Abstract: To study the possibility of increasing the drought tolerance of common bean with the exogenous application of 24-epibrassinolide (EBL), an experiment was conducted in 2016 and 2017. In this experiment, two irrigation levels (optimal irrigation and drought stress) were applied to the main plots and two common bean genotypes (Kusha cultivar and COS16 genotype) and four EBL concentrations (0, 2, 4, and 6 μM) were allocated to sub-plots as factorial. In the flowering stage, drought stress was applied and plants were sprayed with EBL. The results showed that drought stress reduced relative water content (RWC) and increased proline content, malondialdehyde (MDA) content, and antioxidant enzymes activity. However, exogenous application of EBL reduced the seed yield loss and increased the drought stress tolerance in both common bean genotypes by decreasing the MDA content and increasing the RWC, proline content, antioxidant enzymes activity, and nitrate reductase activity. It can be concluded that foliar spray of 4 µM EBL as the best concentration may increase the seed yield and enhance the drought stress tolerance of common bean. Also, Cu/Zn-SOD was up-regulated in response to the drought stress and exogenous EBL. The COS16 genotype showed better response to the drought stress and exogenous EBL than the Kusha cultivar, because of the higher up-regulation of Cu/Zn-SOD in this genotype compared to the Kusha cultivar. Therefore, EBL can be used as a plant growth regulator to enhance drought stress tolerance and minimize the seed yield loss of common bean caused by water deficit.

Photosynthesis under stressful environments: An overview

Abstract: Stressful environments such as salinity, drought, and high temperature (heat) cause alterations in a wide range of physiological, biochemical, and molecular processes in plants. Photosynthesis, the most fundamental and intricate physiological process in all green plants, is also severely affected in all its phases by such stresses. Since the mechanism of photosynthesis involves various components, including photosynthetic pigments and photosystems, the electron transport system, and CO2 reduction pathways, any damage at any level caused by a stress may reduce the overall photosynthetic capacity of a green plant. Details of the stress-induced damage and adverse effects on different types of pigments, photosystems, components of electron transport system, alterations in the activities of enzymes involved in the mechanism of photosynthesis, and changes in various gas exchange characteristics, particularly of agricultural plants, are considered in this review. In addition, we discussed also progress made during the last two decades in producing transgenic lines of different C3 crops with enhanced photosynthetic performance, which was reached by either the overexpression of C3 enzymes or transcription factors or the incorporation of genes encoding C4 enzymes into C3 plants. We also discussed critically a current, worldwide effort to identify signaling components, such as transcription factors and protein kinases, particularly mitogen-activated protein kinases (MAPKs) involved in stress adaptation in agricultural plants.

Heat and Drought Stresses in Crops and Approaches for Their Mitigation.

Abstract: Drought and heat are major abiotic stresses that reduce crop productivity and weaken global food security, especially given the current and growing impacts of climate change and increases in the occurrence and severity of both stress factors. Plants have developed dynamic responses at the morphological, physiological and biochemical levels allowing them to escape and/or adapt to unfavourable environmental conditions. Nevertheless, even the mildest heat and drought stress negatively affects crop yield. Further, several independent studies have shown that increased temperature and drought can reduce crop yields by as much as 50%. Response to stress is complex and involves several factors including signaling, transcription factors, hormones, and secondary metabolites. The reproductive phase of development, leading to the grain production is shown to be more sensitive to heat stress in several crops. Advances coming from biotechnology including progress in genomics and information technology may mitigate the detrimental effects of heat and drought through the use of agronomic management practices and the development of crop varieties with increased productivity under stress. This review presents recent progress in key areas relevant to plant drought and heat tolerance. Furthermore, an overview and implications of physiological, biochemical and genetic aspects in the context of heat and drought are presented. Potential strategies to improve crop productivity are discussed.

Properties and applications of flavonoid metal complexes

Abstract: Flavonoids are widely occurring polyphenol compounds of plant origin with multiple biological and chemical activities. Due to the presence of carbonyl and hydroxyl groups they can coordinate metal ions and form complexes. Metal complexes of flavonoids have many interesting properties: they are colored, often fluorescent, anti- or pro-oxidant, antimicrobial, antiproliferative and biologically active in many other ways. There are many papers covering specific aspects of activity of flavonoid metal complexes, e.g. their antioxidant properties, enzyme-mimicking behavior, therapeutic potential or use in chemical analysis. However, for a researcher interested in this theme, it would be useful to find an extensive review on more than one selected area. Our aim was to cover a wide spectrum of possible activities and potential applications of flavonoids coordinated to metal ions in order to give our readers a broad view on the topic of this class of compounds, their activity and potential applications. While a significant amount of information on the chemical properties and biological activity of flavonoid metal complexes can be found in the literature, an in-depth understanding of structure–property relationships is still lacking. In an attempt to address this issue, a comprehensive discussion of the available data is presented.

Stomatal Conductance Is Essential for Higher Yield Potential of C3 Crops

Abstract: The present review of gas-exchange studies conducted in rainfed or irrigated field conditions clearly indicates that stomatal conductance (gs) of major crops has been inadvertently increased in the last 50 to 80 years as higher yields were being reached through conventional plant breeding. These findings suggest that high gs rates are critical to optimum growth and yield of modern crops, in particular to crop growth rate (CGR) during seed formation and filling. Several hypotheses are presented that may account for this increase. This review also includes studies in which increases in gs were documented for genetically-engineered plants. However, field evaluations of their gas-exchange performance remain sparse. The main finding of this review is that higher rates of gs should be sought, as we forego the usual consideration of crop plants as single organisms losing water, but rather acknowledge that water does exit a crop canopy along a Soil Plant Atmosphere Continuum (SPAC). In non-limiting or moderately-...

Effects of 24‐epibrassinolide on plant growth, antioxidants defense system, and endogenous hormones in two wheat varieties under drought stress

Abstract: Drought stress is a major limitation in enhancing agricultural productivity to fulfill the food demand for the world's population. Fertigation of plants with a variety of biochemicals is being used to create drought resistance in wheat; however, the previous work has been limited in addressing these issues in plants at different growth stages. Therefore, a greenhouse study was conducted to ameliorate the drought stress in two wheat varieties (Chakwal-50 and Faisalabad-2008) by foliar application of 24-epibrassinolide (EBL). It was evident that drought stress had a negative effect on the growth, photosynthesis, and yield of wheat plants. EBL significantly enhanced the plant growth both under optimal and drought conditions. EBL improved the plant height, spike length, and the dry weights of roots, shoots, and grains as compared to control. Furthermore, the foliar application of EBL positively enhanced the osmolyte accumulation, increased the amounts of photosynthetic pigments, and improved the gas exchange parameters. The EBL minimized the oxidative stress by reducing electrolyte leakage, malondialdehyde, and hydrogen peroxide contents whereas it enhanced the activities of antioxidant enzymes, such as catalase, superoxide dismutase, and peroxidase under drought stress. The EBL significantly improved the level of stress hormones, such as abscisic acid, indol acetic acid, and cytokinin under drought stress. The growth response of Chakwal-50 was higher than that of Faisalabad-2008 when exposed to EBL under drought stress. Overall, the EBL plays a major role in the enhancement of growth, biomass, yield, and decrease in oxidative damage in wheat under drought conditions, however; field investigations with different doses of EBL are needed before any further recommendation.