Ahmed A. Eltoukhy

TL;DR: The biological barriers to gene delivery in vivo are introduced and recent advances in material sciences, nanotechnology and nucleic acid chemistry that have yielded promising non-viral delivery systems are discussed, some of which are currently undergoing testing in clinical trials.

TL;DR: In this paper, the authors examined cellular uptake of siRNA delivered in lipid nanoparticles (LNPs) using cellular trafficking probes in combination with automated high-throughput confocal microscopy as well as defined perturbations of cellular pathways paired with systems biology approaches to uncover protein-protein and protein-small molecule interactions.

TL;DR: It is shown that gene silencing assays employing lipoplexes result in a high rate of false negatives that can largely be avoided through formulation, which will facilitate the discovery of next-generation reagents for LNP-mediated nucleic acid delivery.

TL;DR: A magnetic nucleic acid delivery system composed of iron oxide nanoparticles and cationic lipid-like materials termed lipidoids is developed, with performance exceeding that of the commercially available lipid-based reagent, Lipofectamine 2000.

TL;DR: The results help to elucidate the influence of polymer MWD on nucleic acid delivery and provide insight toward the rational design of next-generation materials for gene therapy.