Aki Roberts

Bio: Aki Roberts is an academic researcher from University of Wisconsin–Milwaukee. The author has contributed to research in topics: Poison control & Homicide. The author has an hindex of 15, co-authored 24 publications receiving 1419 citations. Previous affiliations of Aki Roberts include University of New Mexico & University of Aberdeen.

The complete DNA sequence of yeast chromosome III.

Abstract: The entire DNA sequence of chromosome III of the yeast Saccharomyces cerevisiae has been determined. This is the first complete sequence analysis of an entire chromosome from any organism. The 315-kilobase sequence reveals 182 open reading frames for proteins longer than 100 amino acids, of which 37 correspond to known genes and 29 more show some similarity to sequences in databases. Of 55 new open reading frames analysed by gene disruption, three are essential genes; of 42 non-essential genes that were tested, 14 show some discernible effect on phenotype and the remaining 28 have no overt function.

Predictors of Homicide Clearance by Arrest An Event History Analysis of NIBRS Incidents

Abstract: One perspective on homicide clearance by arrest argues that clearance is influenced by police discretion based on victim characteristics. Another suggests that immediate situational characteristics, related to physical evidence and information, are more important in clearing homicides. Using event history analysis, this study examined the effects of victim and situational characteristics on homicide clearance for 1579 murder incidents from the 2002 National Incident-Based Reporting System (NIBRS). Unlike the logistic regression approach typically used in murder clearance research, event history analysis considers the length of time to clearance, increasing the accuracy of estimates. The current analysis found that incidents with female or younger victims were more likely to be cleared. Also, homicides with victims involved in drug- and gang-related activities were more likely to be cleared. However, the significant impact of victim characteristics disappeared after controlling for situational variables re...

Hispanic Victims and Homicide Clearance by Arrest

Abstract: When applied to homicide clearance by arrest, the victim-devaluing perspective posits that police favor higher—social status victims by allocating greater investigative effort to their cases. Previous studies have measured social status via a dichotomous race variable (White vs. Black, White vs. non-White, or non-Hispanic White vs. racial/ ethnic minority). As the Hispanic population grows in the United States, it is increasingly important to extend homicide clearance research beyond the traditional questions of dichotomous racial/ethnic differences. Using 2000-2007 homicide data from agencies that report victim’s ethnicity to the National Incident-Based Reporting System (NIBRS), the current study examines the impact of victim’s race/ethnicity on homicide clearance by arrest via a trichomotous measure (non-Hispanic White, non-Hispanic Black, or Hispanic). Event history analysis found that, controlling for other incident characteristics, homicides with Hispanic victims had a lower risk of clearance by arrest than those with non-Hispanic White or non-Hispanic Black victims.

Explaining japan's postwar violent crime trends*

Abstract: Japan has long been recognized for its low rates of violent crime, rates that usually seem to be declining. The most common explanation for postwar rates links unique cultural characteristics to a system of exceptionally effective informal social controls that, at the macro level, suggest low levels of social disorganization. Other common explanations include low levels of economic stress, a small proportion of young males and a criminal justice system that delivers a high certainty of punishment. In this paper we test these four explanations for Japanese trends using both an annual time-series national analysis (1951 to 2000) and a pooled cross-sectional time-series analysis of the 47 Japanese prefectures from 1955 to 2000 (at 5-year intervals). The results from the two analyses are largely congruent. They show that measures of economic stress, certainty of punishment and age structure are–compared to common social disorganization measures–more consistent predictors of Japanese postwar violent crime trends. Our results suggest that the remarkable strength of the postwar Japanese economy may play a larger role in explaining Japanese violent crime rates than is usually recognized.

Initial sequencing and analysis of the human genome.

Abstract: The human genome holds an extraordinary trove of information about human development, physiology, medicine and evolution. Here we report the results of an international collaboration to produce and make freely available a draft sequence of the human genome. We also present an initial analysis of the data, describing some of the insights that can be gleaned from the sequence.

Life with 6000 Genes

Abstract: The genome of the yeast Saccharomyces cerevisiae has been completely sequenced through a worldwide collaboration. The sequence of 12,068 kilobases defines 5885 potential protein-encoding genes, approximately 140 genes specifying ribosomal RNA, 40 genes for small nuclear RNA molecules, and 275 transfer RNA genes. In addition, the complete sequence provides information about the higher order organization of yeast's 16 chromosomes and allows some insight into their evolutionary history. The genome shows a considerable amount of apparent genetic redundancy, and one of the major problems to be tackled during the next stage of the yeast genome project is to elucidate the biological functions of all of these genes.

Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications.

Abstract: A set of yeast strains based on Saccharomyces cerevisiae S288C in which commonly used selectable marker genes are deleted by design based on the yeast genome sequence has been constructed and analysed. These strains minimize or eliminate the homology to the corresponding marker genes in commonly used vectors without significantly affecting adjacent gene expression. Because the homology between commonly used auxotrophic marker gene segments and genomic sequences has been largely or completely abolished, these strains will also reduce plasmid integration events which can interfere with a wide variety of molecular genetic applications. We also report the construction of new members of the pRS400 series of vectors, containing the kanMX, ADE2 and MET15 genes.

New heterologous modules for classical or PCR‐based gene disruptions in Saccharomyces cerevisiae

Abstract: We have constructed and tested a dominant resistance module, for selection of S. cerevisiae transformants, which entirely consists of heterologous DNA. This kanMX module contains the known kanr open reading-frame of the E. coli transposon Tn903 fused to transcriptional and translational control sequences of the TEF gene of the filamentous fungus Ashbya gossypii. This hybrid module permits efficient selection of transformants resistant against geneticin (G418). We also constructed a lacZMT reporter module in which the open reading-frame of the E. coli lacZ gene (lacking the first 9 codons) is fused at its 3' end to the S. cerevisiae ADH1 terminator. KanMX and the lacZMT module, or both modules together, were cloned in the center of a new multiple cloning sequence comprising 18 unique restriction sites flanked by Not I sites. Using the double module for constructions of in-frame substitutions of genes, only one transformation experiment is necessary to test the activity of the promotor and to search for phenotypes due to inactivation of this gene. To allow for repeated use of the G418 selection some kanMX modules are flanked by 470 bp direct repeats, promoting in vivo excision with frequencies of 10(-3)-10(-4). The 1.4 kb kanMX module was also shown to be very useful for PCR based gene disruptions. In an experiment in which a gene disruption was done with DNA molecules carrying PCR-added terminal sequences of only 35 bases homology to each target site, all twelve tested geneticin-resistant colonies carried the correctly integrated kanMX module.

Genomic Libraries and a Host Strain Designed for Highly Efficient Two-Hybrid Selection in Yeast

Abstract: The two-hybrid system is a powerful technique for detecting protein-protein interactions that utilizes the well-developed molecular genetics of the yeast Saccharomyces cerevisiae. However, the full potential of this technique has not been realized due to limitations imposed by the components available for use in the system. These limitations include unwieldy plasmid vectors, incomplete or poorly designed two-hybrid libraries, and host strains that result in the selection of large numbers of false positives. We have used a novel multienzyme approach to generate a set of highly representative genomic libraries from S. cerevisiae. In addition, a unique host strain was created that contains three easily assayed reporter genes, each under the control of a different inducible promoter. This host strain is extremely sensitive to weak interactions and eliminates nearly all false positives using simple plate assays. Improved vectors were also constructed that simplify the construction of the gene fusions necessary for the two-hybrid system. Our analysis indicates that the libraries and host strain provide significant improvements in both the number of interacting clones identified and the efficiency of two-hybrid selections.