Robotic micromanipulation is a relatively young field. However, after three decades of development and evolution, the fundamental physics; techniques for sensing, actuation, and control; tool sets ...

Robotic Micromanipulation: Fundamentals and Applications

Plasmonic substrates play a crucial role in the confinement and manipulation of localized electromagnetic fields at the nanoscale. The large electromagnetic field enhancement at metal/dielectric interfaces is widely exploited in surface-enhanced fluorescence (SEF) and surface-enhanced Raman scattering (SERS) spectroscopies. Despite the advantage of near-field enhancement, unfortunately, in metals, the large absorption at optical frequencies induces local heating of the analyte fluid with possible damage of the biological material. In addition, in SEF plasmonic substrates, spacer layers are necessary to minimize undesired fluorescence quenching due to nonradiative decay, which strongly depends on the distance between molecules and metallic substrates. Therefore, the possibility of managing surface electromagnetic states mimicking surface-plasmon resonances in terms of spatial localization, high-field intensity, and dispersion characteristics, while avoiding metallic losses is of great interest. However, di...

Surface-Enhanced Raman and Fluorescence Spectroscopy with an All-Dielectric Metasurface

Strategies for protein detection via surface-enhanced Raman spectroscopy (SERS) currently exploit the formation of randomly generated hot spots at the interfaces of metal colloidal nanoparticles, which are clustered together by intrusive chemical or physical processes in the presence of the target biomolecule. We propose a different approach based on selective and quantitative gathering of protein molecules at regular hot spots generated on the corners of individual silver nanocubes in aqueous medium at physiological pH. Here, the protein, while keeping its native configuration, experiences an intense local E-field, which boosts SERS efficiency and detection sensitivity. Uncontrolled signal fluctuations caused by variable molecular adsorption to different particle areas or inside clustered nanoparticles are circumvented. Advanced electron microscopy analyses and computational simulations outline a strategy relying on a site-selective mechanism with superior Raman signal enhancement, which offers the persp...

Site-Selective Surface-Enhanced Raman Detection of Proteins

A Comprehensive Survey on Microgrippers Design: Mechanical Structure

This paper presents a review of electrothermal micro-actuators and applications. Electrothermal micro-actuators have been a significant research interest over the last two decades, and many different designs and applications have been investigated. The electrothermal actuation method offers several advantages when compared with the other types of actuation approaches based on electrostatic and piezoelectric principles. The electrothermal method offers flexibility in the choice of materials, low-cost fabrication, and large displacement capabilities. The three main configurations of electrothermal actuators are discussed: hot-and-cold-arm, chevron, and bimorph types as well as a few other unconventional actuation approaches. Within each type, trends are outlined from the basic concept and design modifications to applications which have been investigated in order to enhance the performance or to overcome the limitations of the previous designs. It provides a grasp of the actuation methodology, design, and fabrication, and the related performance and applications in cell manipulation, micro assembly, and mechanical testing of nanomaterials, Radio Frequency (RF) switches, and optical Micro-Electro-Mechanical Systems (MEMS).

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Review of Electrothermal Actuators and Applications

A Comprehensive Survey on Microgrippers Design: Operational Strategy

Label-free chemical imaging of live cell membranes can shed light on the molecular basis of cell membrane functionalities and their alterations under membrane-related diseases. In principle, this can be done by surface-enhanced Raman scattering (SERS) in confocal microscopy, but requires engineering plasmonic architectures with a spatially invariant SERS enhancement factor G(x, y) = G. To this end, we exploit a self-assembled isotropic nanostructure with characteristics of homogeneity typical of the so-called near-hyperuniform disorder. The resulting highly dense, homogeneous and isotropic random pattern consists of clusters of silver nanoparticles with limited size dispersion. This nanostructure brings together several advantages: very large hot spot density (∼10(4) μm(-2)), superior spatial reproducibility (SD < 1% over 2500 μm(2)) and single-molecule sensitivity (Gav ∼ 10(9)), all on a centimeter scale transparent active area. We are able to reconstruct the label-free SERS-based chemical map of live cell membranes with confocal resolution. In particular, SERS imaging is here demonstrated on red blood cells in vitro in order to use the Raman-resonant heme of the cell as a contrast medium to prove spectroscopic detection of membrane molecules. Numerical simulations also clarify the SERS characteristics of the substrate in terms of electromagnetic enhancement and distance sensitivity range consistently with the experiments. The large SERS-active area is intended for multi-cellular imaging on the same substrate, which is important for spectroscopic comparative analysis of complex organisms like cells. This opens new routes for in situ quantitative surface analysis and dynamic probing of living cells exposed to membrane-targeting drugs.

Surface-enhanced Raman imaging of cell membrane by a highly homogeneous and isotropic silver nanostructure.

Although tissue and cell manipulation nowadays is a common task in biomedical analysis, there are still many different ways to accomplish it, most of which are still not sufficiently general, inexpensive, accurate, efficient or effective. Several problems arise both for in vivo or in vitro analysis, such as the maximum overall size of the device and the gripper jaws (like in minimally-invasive open biopsy) or very limited manipulating capability, degrees of freedom or dexterity (like in tissues or cell-handling operations). This paper presents a new approach to tissue and cell manipulation, which employs a conceptually new conjugate surfaces flexure hinge (CSFH) silicon MEMS-based technology micro-gripper that solves most of the above-mentioned problems. The article describes all of the phases of the development, including topology conception, structural design, simulation, construction, actuation testing and in vitro observation. The latter phase deals with the assessment of the function capability, which consists of taking a series of in vitro images by optical microscopy. They offer a direct morphological comparison between the gripper and a variety of tissues.

Alden Dochshanov

Papers

A Comprehensive Survey on Microgrippers Design: Mechanical Structure

A Comprehensive Survey on Microgrippers Design: Operational Strategy

Surface-enhanced Raman imaging of cell membrane by a highly homogeneous and isotropic silver nanostructure.

Development of Micro-Grippers for Tissue and Cell Manipulation with Direct Morphological Comparison

Compliance Synthesis of CSFH MEMS-Based Microgrippers