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Ales Tichopad
Researcher at Charles University in Prague
Publications - 45
Citations - 6398
Ales Tichopad is an academic researcher from Charles University in Prague. The author has contributed to research in topics: Medicine & Gene expression. The author has an hindex of 20, co-authored 37 publications receiving 5694 citations. Previous affiliations of Ales Tichopad include Academy of Sciences of the Czech Republic & Czech Technical University in Prague.
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Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper--Excel-based tool using pair-wise correlations.
TL;DR: The developed, and herein presented, software called BestKeeper determines the best suited standards, out of ten candidates, and combines them into an index, which can be compared with further target genes to decide, whether they are differentially expressed under an applied treatment.
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Standardized determination of real‐time PCR efficiency from a single reaction set‐up
TL;DR: The developed method yields results of higher accuracy than the currently used method of serial dilutions for amplification efficiency estimation, and resists the subjective influence of researchers, and the estimation can therefore be fully instrumentalized.
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How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments.
TL;DR: Recommendations for the precise estimation of PCR efficiency are proposed: one robust standard curve with at least 3–4 qPCR replicates at each concentration shall be generated, and using a larger volume when constructing serial dilution series reduces sampling error and enables calibration across a wider dynamic range.
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Genetic variability in the SNCA gene influences alpha-synuclein levels in the blood and brain.
Julia Fuchs,Julia Fuchs,Ales Tichopad,Yulia Golub,Marita Munz,Katherine Schweitzer,Bjoern Wolf,Daniela Berg,Jakob C. Mueller,Thomas Gasser +9 more
TL;DR: Evidence is provided that a‐synuclein levels are influenced by genetic variability in the promoter and 3′ region of the SNCA gene in vivo, and the “protective” genotype 259/259 of the PD‐associated promoter repeat NACP‐Repl is associated with lower protein levels in blood.
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Inhibition of real-time RT-PCR quantification due to tissue-specific contaminants.
TL;DR: It is observed that unknown tissue-specific factors can influence amplification kinetics but this affect can be ameliorated, in part, by appropriate primer selection.