Showing papers by "Alexander N. Glazer published in 1972"
TL;DR: The auramine O-alcohol dehydrogenase interaction offers an additional case to the general correlation that most dyes which bind tightly to enzymes interact at regions overlapping binding sites for the normal ligands.
27 citations
TL;DR: Th Thin fibers, which appeared to originate from only one end of the flagellar filament, were formed upon trypsin digestion, and the amino acid composition of the purified fibers was very different from that of intact Salmonella flageLLin, andThe fibers did not cross-react with antiflagellin or antif ladellar antiserum.
Abstract: Sheared flagella of Salmonella typhimurium strains SL 870 (Nml(+)Fla(+)) and SL 871 (Nml(-)Fla(+)) were found to be susceptible to proteolytic digestion by trypsin, chymotrypsin, and Pronase. The rate of tryptic digestion was similar for the epsilon-N-methyllysine-containing and the nonmethylated flagella. Thin fibers, which appeared to originate from only one end of the flagellar filament, were formed upon trypsin digestion. The fibers were not dissociated at extremes of pH or upon heating. The amino acid composition of the purified fibers was very different from that of intact Salmonella flagellin, and the fibers did not cross-react with antiflagellin or antiflagellar antiserum. The possible significance of these findings is discussed in relation to the flagellar structure.
10 citations