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Ali Bordbar

Bio: Ali Bordbar is an academic researcher from Pasteur Institute of Iran. The author has contributed to research in topics: Leishmania major & Leishmania. The author has an hindex of 9, co-authored 20 publications receiving 189 citations. Previous affiliations of Ali Bordbar include Mashhad University of Medical Sciences.

Papers
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Journal ArticleDOI
TL;DR: The statistically Fst values indicate that Echinococcus sensu stricto populations are not genetically well differentiated in various geographical regions of Iran, and further study is needed to analyze concatenated mitogenomes.

50 citations

Journal ArticleDOI
TL;DR: The analytical framework conveys a clear understanding of both L. major and L. turanica which can only be approved as causative agents of ZCL by more extensive sampling and followed by standardized molecular diagnosis.

21 citations

Journal ArticleDOI
TL;DR: The objective was to find Leishmania parasites circulating in reservoir hosts, sand flies and human simultaneously and to describe the mechanisms leading to ZCL in Iran.
Abstract: Objectives Only Leishmania major is well known as a causative agent of zoonotic cutaneous leishmaniasis (ZCL) in Iran. Our objective was to find Leishmania parasites circulating in reservoir hosts, sand flies and human simultaneously. Methods Sand flies, rodents and prepared smears of humans were sampled. DNA of Leishmania parasites was extracted, and two fragments of ITS-rDNA gene amplified by PCR. RFLP and sequencing were employed to identify Leishmania parasites. Results Leishmania major and L. turanica were identified unequivocally by targeting and sequencing ITS-rDNA from humans, rodents and sand flies. The new Leishmania species close to gerbilli (GenBank Accession Nos. EF413076; EF413087) was discovered only in sand flies. Conclusion Based on parasite detection of ITS-rDNA in main and potential reservoir hosts and vectors and humans, we conclude that at least two Leishmania species are common in the Turkmen Sahra ZCL focus. Phylogenetic analysis proved that the new Leishmania is closely related to Leishmania mammal parasites (Leishmania major, Leishmania turanica, Leishmania gerbilli). Its role as a principal agent of ZCL is unknown because it was found only in sand flies. Our findings shed new light on the transmission cycles of several Leishmania parasites in sand flies, reservoir hosts and humans. Objectifs Seul Leishmania major est bien connu comme agent causal de la leishmaniose cutanee zoonotique (LCZ) en Iran. Notre objectif etait de trouver des parasites de Leishmania circulant simultanement chez les hotes reservoirs, les phlebotomes et les humains. Methodes Les phlebotomes, les rongeurs et des frottis prepares a partir d'humains ont ete echantillonnes. L’ADN de Leishmania a ete extrait et deux fragments du gene ITS-ADNr ont ete amplifies par PCR. Le RFLP et le sequencage ont ete utilises pour identifier les parasites Leishmania. Resultats L. major et L. turanica ont ete identifies sans equivoque par le ciblage et le sequencage de l’ITS-ADNr chez les etres humains, les rongeurs et les phlebotomes. Nous avons decouvert une nouvelle espece de Leishmania chez les phlebotomes que nous avons nomme L. iranica (n° d'acces GenBank: EF413076. EF413087). Conclusion Sur base de la detection des parasites par l’ITS-ADNr chez les hotes reservoirs principaux et potentiels et les vecteurs et les humains, nous concluons qu'au moins deux especes de Leishmania sont courantes dans le foyer Turkemen Sahara ZCL. L'analyse phylogenetique a montre que la nouvelle espece Leishmania est etroitement liee a des parasites Leishmania de mammiferes (L. major, L. turanica, L. gerbilli). Son role en tant que principal agent de LCZ est inconnu car elle n'a ete retrouvee que dans les phlebotomes. Nos resultats apportent une nouvelle lumiere sur les cycles de transmission de plusieurs parasites Leishmania dans les phlebotomes, les hotes reservoirs et les humains. Objetivos Solo Leishmania major es un agente causal bien conocido de la Leishmaniasis cutanea zoonotica (LCZ) en Iran. Nuestro objetivo era encontrar parasitos de Leishmania circulando simultaneamente entre hospederos reservorios, moscas de arena y humanos. Metodos Se tomaron muestras de moscas de arena, roedores y frotis preparados de humanos. Se extrajo el ADN de Leishmania y mediante PCR se amplificaron dos fragmentos del gen ITS-ADNr. Se utilizaron el RFLP y la secuenciacion para identificar los parasitos de Leishmania. Resultados Se identifico a L. major y L. turanica de forma inequivoca mediante amplificacion y secuenciacion del ITS-ADNr de muestras de humanos, roedores y moscas de arena. En moscas, hemos descubierto una nueva especie de Leishmania, la cual hemos llamado L. iranica (numeros de acceso de GenBank EF413076; EF413087). Conclusion Basandonos en la deteccion del ITS-ADNr de parasitos en el hospedero principal y otros potenciales, en vectores y en humanos, concluimos que al menos dos especies de Leishmania son comunes en el foco de LCZ de Turkemen Sahara. El analisis filogenetico muestra que la nueva Leishmania esta estrechamente relacionada con especies de Leishmania que parasitan mamiferos (L. major, L. turanica, L. gerbilli). Su papel como el agente principal de LCZ es desconocido puesto que se encontro solamente en moscas de arena. Nuestros hallazgos aportan nuevos datos sobre los ciclos de transmision de varios parasitos de Leishmania en moscas de arena, hospederos reservorios y humanos.

20 citations

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TL;DR: Finding P. sergenti revealed the probability of transmission and distribution patterns of a non-native vector of CL in related zones and could be used as models indicating climatic zones and environmental variables connected to sand fly presence and vector distribution.
Abstract: Cutaneous leishmaniasis (CL) is a complex vector-borne disease caused by Leishmania parasites that are transmitted by the bite of several species of infected female phlebotomine sand flies. Monthly factor analysis of climatic variables indicated fundamental variables. Principal component-based regionalization was used for recognition of climatic zones using a clustering integrated method that identified five climatic zones based on factor analysis. To investigate spatial distribution of the sand fly species, the kriging method was used as an advanced geostatistical procedure in the ArcGIS modeling system that is beneficial to design measurement plans and to predict the transmission cycle in various regions of Khuzestan province, southwest of Iran. However, more than an 80% probability of P. papatasi was observed in rainy and temperate bio-climatic zones with a high potential of CL transmission. Finding P. sergenti revealed the probability of transmission and distribution patterns of a non-native vector of CL in related zones. These findings could be used as models indicating climatic zones and environmental variables connected to sand fly presence and vector distribution. Furthermore, this information is appropriate for future research efforts into the ecology of Phlebotomine sand flies and for the prevention of CL vector transmission as a public health priority.

19 citations

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TL;DR: It is concluded that one species of sandfly can be infected with different Wolbachia strains and that differentspecies of sandflies can beinfected with a common strain.
Abstract: Individual, naturally occurring Phlebotomus mongolensis and Phlebotomus caucasicus from Iran were screened for infections with the maternally inherited intracellular Rickettsia-like bacterium Wolbachia pipientis via targeting a major surface protein gene (wsp). The main objective of this study was to determine if W. pipientis could be detected in these species. The sandflies were screened using polymerase chain reaction to amplify a fragment of the Wolbachia surface protein gene. The obtained sequences were edited and aligned with database sequences to identify W. pipientis haplotypes. Two strains of Wolbachia were found. Strain Turk 54 (accession EU780683) is widespread and has previously been reported in Phlebotomus papatasi and other insects. Strain Turk 07 (accession KC576916) is a novel strain, found for first time in the two sister species. A-group strains of W. pipientis occur throughout much of the habitat of these sandflies. It is possible that Wolbachia is transferred via horizontal transmission. Horizontal transfer could shed light on sandfly control because Wolbachia is believed to drive a deleterious gene into sandflies that reduces their natural population density. With regard to our findings in this study, we can conclude that one species of sandfly can be infected with different Wolbachia strains and that different species of sandflies can be infected with a common strain.

18 citations


Cited by
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Journal ArticleDOI
TL;DR: A classification method based on the suitability of well-studied molecular markers for typing the 21 known Leishmania species pathogenic to humans is proposed, which can be applied to newly discovered species and to hybrid strains originating from inter-species crosses.

196 citations

Journal ArticleDOI
15 Jun 2017-Parasite
TL;DR: The present review offers information for an integrated control programme against economically relevant parasites of camels in Iran from 1931 to February 2017.
Abstract: Parasitic diseases of camels are major causes of impaired milk and meat production, decreases in performance or even death. Some camel parasites also represent a threat to human health. About 171,500 one-humped camels (Camelus dromedarius) and 100-300 two-humped camels (Camelus bactrianus) live in Iran. Knowledge of the biodiversity of their parasites is still limited. The present review covers all information about camel parasitic diseases in Iran published as dissertations and in both Iranian and international journals from 1931 to February 2017. Ten genera of Protozoa (Trypanosoma, Eimeria, Cryptosporidium, Toxoplasma, Neospora, Sarcocystis, Besnoitia, Theileria, Babesia and Balantidium), 48 helminth species detected in the digestive system, including three species of Trematoda, four species of Cestoda, and 41 species of Nematoda, as well as helminths from other organs - Echinococcus spp., Dictyocaulus filaria, Thelazia leesei, Dipetalonema evansi and Onchocerca fasciata - have so far been described in Iranian camels. Furthermore, 13 species of hard ticks, mange mites, the myiasis flies Cephalopina titillator and Wohlfahrtia magnifica, and immature stages of the Pentastomida Linguatula serrata have also been reported from camels of Iran. Camel parasitic diseases are a major issue in Iran in terms of economics and public health. The present review offers information for an integrated control programme against economically relevant parasites of camels.

84 citations

Journal ArticleDOI
TL;DR: Observations suggest that LAMP offers a good molecular POC technique for the diagnosis of leishmaniasis and is also readily applicable to screening at-risk populations and vector sand flies for Leishmania infection in endemic areas.
Abstract: Leishmaniasis, caused by protozoan parasites of the Leishmania genus, represents an important health problem in many regions of the world. Lack of effective point-of-care (POC) diagnostic tests applicable in resources-limited endemic areas is a critical barrier to effective treatment and control of leishmaniasis. The development of the loop-mediated isothermal amplification (LAMP) assay has provided a new tool towards the development of a POC diagnostic test based on the amplification of pathogen DNA. LAMP does not require a thermocycler, is relatively inexpensive, and is simple to perform with high amplification sensitivity and specificity. In this review, we discuss the current technical developments, applications, diagnostic performance, challenges, and future of LAMP for molecular diagnosis and surveillance of Leishmania parasites. Studies employing the LAMP assay to diagnose human leishmaniasis have reported sensitivities of 80% to 100% and specificities of 94% to 100%. These observations suggest that LAMP offers a good molecular POC technique for the diagnosis of leishmaniasis and is also readily applicable to screening at-risk populations and vector sand flies for Leishmania infection in endemic areas.

78 citations

Journal ArticleDOI
TL;DR: Comprehensive molecular studies that could show the species and strains of Leishmania in different areas of each country can give a better view from the distribution of CL in this region.
Abstract: Leishmania tropica and Leishmania major are both the main cause of anthroponotic (ACL) and zoonotic cutaneous leishmaniasis (ZCL), respectively, in the Old World. Leishmania infantum and Leishmania donovani, which are important causes of visceral leishmaniasis, have also occasionally been reported in CL patients. The present study investigates the current distribution of causative species of CL in Iran and neighboring countries in the Middle East. There has been expansion of L. tropica into new urban and rural foci in Iran, with well-documented cases of visceralization, a substantial increase of CL in Syria, and the emergence of new foci and outbreaks in Turkey and Iraq, especially due to L. major. Civil war in Syria and Iraq, population movement, poverty, and climatic change play important roles in the changing CL distribution in this region. Control programs should adopt a multidisciplinary approach based on active surveillance and case finding, especially in vulnerable refugee populations, determination of hazard maps for CL hot points using GIS and other advanced technology, the free distribution of drugs, rodent control, and greater community engagement in poor and marginalized populations. Comprehensive molecular studies that could show the species and strains of Leishmania in different areas of each country can give a better view from the distribution of CL in this region.

66 citations

Journal ArticleDOI
TL;DR: This is the first study to predict the distribution of ZCL reservoir hosts in Iran, and climbers and topography variables had high contributions toward the prediction of potential distribution of the main reservoir species; therefore, as climate changes, the models should be updated periodically with novel data, and the results should be used in disease-monitoring programs.

64 citations