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Showing papers by "Ali H. Bahkali published in 2001"


Journal ArticleDOI
01 Nov 2001-Mycoses
TL;DR: Overall, 13% of patients reacted positively to fungal extracts, revealing allergic sensitization to these fungi, and necessitate further investigation as regards the purification and characterization of these local extracts for better diagnostic use in patients in Saudi Arabia.
Abstract: Summary. The allergenicity and antigenicity of various airborne fungi isolated from the atmosphere of Riyadh were studied. Protein nitrogen contents were estimated and found to range from 0.9 mg ml−1 for Cladosporium to 2.1 mg ml−1 for Aspergillus extracts. Sodium dodecyl sulphate–polyacrylamide gel electrophoresis analysis for those extracts exhibited a number of protein bands of higher molecular weight between 13 and 80 kDa for Alternaria, Ulocladium, Penicillium, Aspergillus and Cladosporium. Extracts in both aqueous and lyophilized forms were sterilized and tested for diagnostic skin prick test in 100 consecutive patients having bronchial asthma and allergic rhinitis. Overall, 13% of patients reacted positively to fungal extracts, revealing allergic sensitization to these fungi. These findings necessitate further investigation as regards the purification and characterization of these local extracts for better diagnostic use in patients in Saudi Arabia. Zusammenfassung. Es wurde die Allergenitat und Antigenitat mehrerer luftburtiger Pilze untersucht, die aus der Luft von Riad isoliert worden waren. Hierzu wurden Pilzextrakte hergestellt, deren Proteinstickstoffgehalt zwischen 0.9 mg ml−1 bei Cladosporium und 2.1 mg ml−1 bei Aspergillus lag. Die SDS–PAGE-Analyse zeigte eine Anzahl von Proteinbanden hoheren Molekulargewichts zwischen 13 und 80 kDa fur Alternaria, Ulocladium, Penicillium, Aspergillus und Cladosporium. Die sowohl wassrigen wie lyophilisierten Extrakte wurden sterilisiert und an 100 unausgewahlten Patienten mit Bronchialasthma und allergischer Rhinitis im Pricktest getestet. Ingesamt 13% der Patienten reagierten auf die Extrakte positiv, was fur eine allergische Sensibilisierung gegen diese Pilze spricht.

11 citations