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Showing papers by "Ali H. Bahkali published in 2018"


Journal ArticleDOI
TL;DR: Novel fungal taxa are described in the present study, including 17 new genera, 93 new species, four combinations, a sexual record for a species and new host records for 16 species.
Abstract: This paper is the seventh in the Fungal Diversity Notes series, where 131 taxa accommodated in 28 families are mainly described from Rosa (Rosaceae) and a few other hosts. Novel fungal taxa are described in the present study, including 17 new genera, 93 new species, four combinations, a sexual record for a species and new host records for 16 species. Bhatiellae, Cycasicola, Dactylidina, Embarria, Hawksworthiana, Italica, Melanocucurbitaria, Melanodiplodia, Monoseptella, Uzbekistanica, Neoconiothyrium, Neopaucispora, Pararoussoella, Paraxylaria, Marjia, Sporormurispora and Xenomassariosphaeria are introduced as new ascomycete genera. We also introduce the new species Absidia jindoensis, Alternaria doliconidium, A. hampshirensis, Angustimassarina rosarum, Astragalicola vasilyevae, Backusella locustae, Bartalinia rosicola, Bhatiellae rosae, Broomella rosae, Castanediella camelliae, Coelodictyosporium rosarum, Comoclathris rosae, C. rosarum, Comoclathris rosigena, Coniochaeta baysunika, C. rosae, Cycasicola goaensis, Dactylidina shoemakeri, Dematiopleospora donetzica, D. rosicola, D. salsolae, Diaporthe rosae, D. rosicola, Endoconidioma rosae-hissaricae, Epicoccum rosae, Hawksworthiana clematidicola, H. lonicerae, Italica achilleae, Keissleriella phragmiticola, K. rosacearum, K. rosae, K. rosarum, Lophiostoma rosae, Marjia tianschanica, M. uzbekistanica, Melanocucurbitaria uzbekistanica, Melanodiplodia tianschanica, Monoseptella rosae, Mucor fluvius, Muriformistrickeria rosae, Murilentithecium rosae, Neoascochyta rosicola, Neoconiothyrium rosae, Neopaucispora rosaecae, Neosetophoma rosarum, N. rosae, N. rosigena, Neostagonospora artemisiae, Ophiobolus artemisiicola, Paraconiothyrium rosae, Paraphaeosphaeria rosae, P. rosicola, Pararoussoella rosarum, Parathyridaria rosae, Paraxylaria rosacearum, Penicillium acidum, P. aquaticum, Phragmocamarosporium rosae, Pleospora rosae, P. rosae-caninae, Poaceicola agrostina, P. arundinicola, P. rosae, Populocrescentia ammophilae, P. rosae, Pseudocamarosporium pteleae, P. ulmi-minoris, Pseudocercospora rosae, Pseudopithomyces rosae, Pseudostrickeria rosae, Sclerostagonospora lathyri, S. rosae, S. rosicola, Seimatosporium rosigenum, S. rosicola, Seiridium rosarum, Setoseptoria arundelensis, S. englandensis, S. lulworthcovensis, Sigarispora agrostidis, S. caryophyllacearum, S. junci, S. medicaginicola, S. rosicola, S. scrophulariae, S. thymi, Sporormurispora atraphaxidis, S. pruni, Suttonomyces rosae, Umbelopsis sinsidoensis, Uzbekistanica rosae-hissaricae, U. yakutkhanika, Wojnowicia rosicola, Xenomassariosphaeria rosae. New host records are provided for Amandinea punctata, Angustimassarina quercicola, Diaporthe rhusicola, D. eres, D. foeniculina, D. rudis, Diplodia seriata, Dothiorella iberica, Lasiodiplodia theobromae, Lecidella elaeochroma, Muriformistrickeria rubi, Neofusicoccum australe, Paraphaeosphaeria michotii, Pleurophoma pleurospora, Sigarispora caulium and Teichospora rubriostiolata. The new combinations are Dactylidina dactylidis (=Allophaeosphaeria dactylidis), Embarria clematidis (=Allophaeosphaeria clematidis), Hawksworthiana alliariae (=Dematiopleospora alliariae) and Italica luzulae (=Dematiopleospora luzulae). This study also provides some insights into the diversity of fungi on Rosa species and especially those on Rosa spines that resulted in the characterisation of eight new genera, 45 new species, and nine new host records. We also collected taxa from Rosa stems and there was 31% (20/65) overlap with taxa found on stems with that on spines. Because of the limited and non-targeted sampling for comparison with collections from spines and stems of the same host and location, it is not possible to say that the fungi on spines of Rosa differ from those on stems. The study however, does illustrate how spines are interesting substrates with high fungal biodiversity. This may be because of their hard structure resulting in slow decay and hence are suitable substrates leading to fungal colonisation. All data presented herein are based on morphological examination of specimens, coupled with phylogenetic sequence data to better integrate taxa into appropriate taxonomic ranks and infer their evolutionary relationships.

151 citations


Journal ArticleDOI
TL;DR: In this article, the authors describe the development of e-caprolactone-citric acid nanomicelles using deep eutectic solvents (DESs) in the form of choline chloride and citric acid.
Abstract: Biomaterials developed using sustainable methods and non-toxic solvents have been effectively applied as eco-friendly, sustainable reaction medium and catalysts for biological applications. This study describes the development of e-caprolactone-citric acid nanomicelles using deep eutectic solvents (DESs) in the form of choline chloride and citric acid. The solvent formation was confirmed by FT-IR and 1H NMR spectroscopy. The highest polycondensation yield of e-caprolactone (e-cp) with citric acid (CA) poly(e-cp-co-CA) was obtained in 1 : 2 DES medium. Camptothecin (CPT), used as a model anticancer agent, was encapsulated by poly(e-cp-co-CA) micelles, which act as drug carriers. Micelle formation was determined by fluorescence analysis using pyrene (CMC = 0.5 mg mL−1). The self-assembled CPT-loaded poly(e-cp-co-CA) micelles were characterized using FT-IR, XRD, SEM and TEM techniques. The size of the nanomicelles was observed to be ∼270 nm using dynamic light scattering (DLS) techniques. The encapsulation efficiency and in vitro profiles of CPT in poly(e-cp-co-CA) micelles were investigated by UV spectrometry at λmax of 350 nm. The antiproliferative effect of the encapsulated CPT, which was investigated in A549 cells, suggested that e-cp-co-CA may be a promising anticancer drug carrier.

27 citations


Journal ArticleDOI
TL;DR: Maximum likelihood and Bayesian inference analyses of a combined ITS, LSU and RPB2 DNA sequence dataset show that V. yunnana is a new species, belonging to the genus Vamsapriya in Xylariaceae.
Abstract: Vamsapriya yunnana sp. nov., collected from dead bamboo in Yunnan Province of China, is described and illustrated in this paper. The new taxon is characterized by synnematous conidiophores, with enteroblastic, monotretic conidiogenous cells and brown to dark brown, 4–9-septate, fusiform conidia with a long, apical, appendage-like region. The species differs from the type and other species in the genus in having a wider fertile region and rostrate apex of the conidia. Maximum likelihood and Bayesian inference analyses of a combined ITS, LSU and RPB2 DNA sequence dataset show that V. yunnana is a new species, belonging to the genus Vamsapriya in Xylariaceae.

11 citations


Journal ArticleDOI
TL;DR: A high incidence of Brucella in camels of Riyadh suggests an important health issue associated with consumption of raw camel milk, and a modified method for the isolation of DNA from camel milk is described.
Abstract: Brucellosis a zoonotic disease is endemic in Saudi Arabia especially in the Najed region due to the customary consumption of raw milk. For a detailed investigation of brucellosis in camel milk, 80 samples were collected from Riyadh region. Twenty-four and sixteen percentage of the samples were positive for Brucella when tested with Rose Bengal test and ELISA, respectively. Genomic DNA from the milk samples was prepared using a modified method presented in this study. Brucella was detected in camel milk using three different primer sets targeting Brucella 16S rRNA gene, omp2, and IS711 genes. Among the three primer sets, an F4/R2 set of primers targeting Brucella 16S rRNA gene was most sensitive and yielded PCR products from nine milk samples. Finally, the 16S rRNA gene sequences of these isolates confirmed the contamination of Brucella spp. in nine milk samples. This study, therefore, suggests a high incidence of Brucella in camels of Riyadh. Practical applications This manuscript describes a modified method for the isolation of DNA from camel milk. The DNA was subsequently used successfully for the amplification of various genes for detecting Brucella. Furthermore, there was no report from Riyadh region on the detection of Brucella from camel milk. This report, therefore, points out toward an important health issue associated with consumption of raw camel milk. If published, this report will be helpful in educating the local population about the health risk associated with the consumption of raw milk.

7 citations


Journal ArticleDOI
01 Mar 2018-Apmis
TL;DR: It is suggested that consumption of raw camel milk from this region constitutes a great health risk because many strains exhibit β‐hemolytic activity and resistant to six different antibiotics.
Abstract: Customary consumption of unpasteurized milk by the population in the central Najed region of Saudi Arabia may pose a health risk. Therefore, 80 camel milk samples were collected aseptically from seven different stations of Riyadh region. The biochemical and microbiological properties of these milk samples were determined. Nutrient agar and brain heart infusion agar were used to determine mesophilic aerobic counts (MACs). The MAC in each mL of milk varied from 60 to 16 × 104 CFU/mL on nutrient agar. Based on the colony morphology, 176 colonies were collected from different samples, and these isolates were de-replicated into 80 unique isolates using rep-PCR analysis. Surprisingly, the 16S rRNA sequence analysis of these strains revealed that more than one-third of the collected milk samples contained strains that share maximum sequence similarities with well-known pathogens, such as Brucella, Bacillus anthracis, Listeria monocytogenes, and MRSA. Furthermore, many strains exhibit 16S rRNA gene similarity with opportunistic pathogens such as Citrobacter freundii and Kytococcus schroeteri. Many strains exhibit β-hemolytic activity and resistant to six different antibiotics. Our study suggested that consumption of raw camel milk from this region constitutes a great health risk.

6 citations


Journal ArticleDOI
TL;DR: In this study, monodispersed, highly biocompatible and substantially stable glucose encapsulated CdO nanoparticles (G-CdO NPs) of uniform sizes were synthesized using a sol-gel route and the potencies of these NPs against gram-negative and gram-positive multi-drug resistant (MDR) bacteria were investigated.

5 citations


Journal ArticleDOI
TL;DR: A new species of Neolinocarpon, N. phayaoense, was found on a branch of Hevea brasiliensis in northern Thailand, with evidence from morphology and phylogenetic analyses confirming the phylogenetic affinity in the species.
Abstract: A new species of Neolinocarpon , N. phayaoense was found on a branch of Hevea brasiliensis in northern Thailand . The new species is introduced in this paper, with evidence from morphology and phylogenetic analyses . Descriptions, illustrations and notes are provided for the new taxon. Maximum - likelihood and Bayesian inference analyses of a combined LSU and ITS sequence data clarified the phylogenetic affinity in Neolinocarpon , with the species separated from Linocarpon in Linocarpaceae . Neolinocarpon is morphologically distinct from Linocarpon in having immersed ascomata.

5 citations