Ali H. Bahkali

Bio: Ali H. Bahkali is an academic researcher from King Saud University. The author has contributed to research in topics: Dothideomycetes & Pleosporales. The author has an hindex of 50, co-authored 287 publications receiving 9411 citations. Previous affiliations of Ali H. Bahkali include Yeungnam University & Landcare Research.

Bacterial isolates exhibiting multidrug resistance, hemolytic activity, and high 16S rRNA gene similarity with well-known pathogens found in camel milk samples of Riyadh region.

Abstract: Customary consumption of unpasteurized milk by the population in the central Najed region of Saudi Arabia may pose a health risk. Therefore, 80 camel milk samples were collected aseptically from seven different stations of Riyadh region. The biochemical and microbiological properties of these milk samples were determined. Nutrient agar and brain heart infusion agar were used to determine mesophilic aerobic counts (MACs). The MAC in each mL of milk varied from 60 to 16 × 104 CFU/mL on nutrient agar. Based on the colony morphology, 176 colonies were collected from different samples, and these isolates were de-replicated into 80 unique isolates using rep-PCR analysis. Surprisingly, the 16S rRNA sequence analysis of these strains revealed that more than one-third of the collected milk samples contained strains that share maximum sequence similarities with well-known pathogens, such as Brucella, Bacillus anthracis, Listeria monocytogenes, and MRSA. Furthermore, many strains exhibit 16S rRNA gene similarity with opportunistic pathogens such as Citrobacter freundii and Kytococcus schroeteri. Many strains exhibit β-hemolytic activity and resistant to six different antibiotics. Our study suggested that consumption of raw camel milk from this region constitutes a great health risk.

Life-history Traits of the Blacktrip sardinella, Sardinella melanura (Clupeidae) in the Gwadar, Balochistan Coast, Pakistan

Abstract: The present study describes the first complete and comprehensive description on life-history traits of Sardinella melanura including length-frequency distributions (LFDs), length-weight relationships (LWRs), condition factors (allometric condition, KA; Fulton’s condition, KF; relative condition, KR), and relative weight (WR) in the Gwadar, Balochistan Coast, Pakistan. Additionally, form factor (a3.0) using available a and b regression parameters of LWRs and size at first sexual maturity (Lm) using available maximum length for 07 Sardinella spp. in 23 water-bodies were calculated. Seasonal samples of S. melanura were collected from the Gwadar, Balochistan Coast, Pakistan using gill nets during July 2013 to J u n e 2014. Total length (TL) was measured to nearest 0.1 cm using digital slide calipers and total body weight (BW) was measured using an electronic balance with 0.1 g accuracy for each individual. A total of 600 individuals of S. melanura were analyzed, where minimum and maximum TL was 12.5 cm and 23.00 cm, respectively and BW was 16.5g and 114.8 g, correspondingly. Highest number (25.83%) of its population stands at 17.00 cm to 18.00 cm size group. The b value of LWR indicated negative allometric growth in the population. All the condition factors (KA, KF, KR, and WR) were significantly correlated with TL and BW (P < 0.001). WR showed significant difference from 100 (P < 0.001) representing imbalanced population in presence of prey and predator. In addition, the a3.0 for S. melanura was 0.0078. The size at first sexual maturity (Lm) was 14.43 cm in TL of S. melanura in the Gwadar, Balochistan coast, Pakistan.

Sexual morph of Seimatosporium cornii found on Cornus sanguinea in Italy

Abstract: The sexual morph of Seimatosporium cornii , collected from dead stems of Cornus sanguinea in Arezzo, Italy, is reported in this paper. It was linked to S. cornii based on analysis of combined LSU and ITS sequence data . The sexual morph is characterized by minutely stromatic ascomata, cylindrical to clavate asci, with a J+, apical apparatus and 1–3-septate, fusiform ascospores. Seimatosporium cornii can be distinguished from the closely related species, Discostroma fuscellum , by the structure of the peridium, shape and septation of the ascospores and also its asexual morph. The sexual morph of S. cornii is reported from the same host and location as its asexual morph. We also provide data of SSU, TEF1-α and β-TUB genes of our strain, deposited in GenBank.

A new species

Abstract: We redescribe the only previously known species of Myrsidea from bulbuls, M. pycnonoti Eichler. Sixteen new species are described; they and their type hosts are: M. phillipsi ex Pycnonotus goiavier goiavier (Scopoli), M. gieferi ex P. goiavier suluensis Mearns, M. kulpai ex P. flavescens Blyth, M. finlaysoni ex P. finlaysoni Strickland, M. kathleenae ex P. cafer (L.), M. warwicki ex Ixos philippinus (J. R. Forster), M. mcclurei ex Microscelis amaurotis (Temminck), M. zeylanici ex P. zeylanicus (Gmelin), M. plumosi ex P. plumosus Blyth, M. eutiloti ex P. eutilotus (Jardine and Selby), M. adamsae ex P. urostictus (Salvadori), M. ochracei ex Criniger ochraceus F. Moore, M. borbonici ex Hypsipetes borbonicus (J. R. Forster), M. johnsoni ex P. atriceps (Temminck), M. palmai ex C. ochraceus, and M. claytoni ex P. eutilotus. A key is provided for the identification of these 17 species.

Ab initio calculation of vibrational absorption and circular dichroism spectra using density functional force fields

Abstract: : The unpolarized absorption and circular dichroism spectra of the fundamental vibrational transitions of the chiral molecule, 4-methyl-2-oxetanone, are calculated ab initio. Harmonic force fields are obtained using Density Functional Theory (DFT), MP2, and SCF methodologies and a 5S4P2D/3S2P (TZ2P) basis set. DFT calculations use the Local Spin Density Approximation (LSDA), BLYP, and Becke3LYP (B3LYP) density functionals. Mid-IR spectra predicted using LSDA, BLYP, and B3LYP force fields are of significantly different quality, the B3LYP force field yielding spectra in clearly superior, and overall excellent, agreement with experiment. The MP2 force field yields spectra in slightly worse agreement with experiment than the B3LYP force field. The SCF force field yields spectra in poor agreement with experiment.The basis set dependence of B3LYP force fields is also explored: the 6-31G* and TZ2P basis sets give very similar results while the 3-21G basis set yields spectra in substantially worse agreements with experiment. jg

A European Database of Fusarium graminearum and F. culmorum Trichothecene Genotypes

Abstract: Fusarium species, particularly Fusarium graminearum and F. culmorum, are the main cause of trichothecene type B contamination in cereals. Data on the distribution of Fusarium trichothecene genotypes in cereals in Europe are scattered in time and space. Furthermore, a common core set of related variables (sampling method, host cultivar, previous crop, etc.) that would allow more effective analysis of factors influencing the spatial and temporal population distribution, is lacking. Consequently, based on the available data, it is difficult to identify factors influencing chemotype distribution and spread at the European level. Here we describe the results of a collaborative integrated work which aims (1) to characterize the trichothecene genotypes of strains from three Fusarium species, collected over the period 2000–2013 and (2) to enhance the standardization of epidemiological data collection. Information on host plant, country of origin, sampling location, year of sampling and previous crop of 1147 F. graminearum, 479 F. culmorum, and 3 F. cortaderiae strains obtained from 17 European countries was compiled and a map of trichothecene type B genotype distribution was plotted for each species. All information on the strains was collected in a freely accessible and updatable database (www.catalogueeu.luxmcc.lu), which will serve as a starting point for epidemiological analysis of potential spatial and temporal trichothecene genotype shifts in Europe. The analysis of the currently available European dataset showed that in F. graminearum, the predominant genotype was 15-acetyldeoxynivalenol (15-ADON) (82.9%), followed by 3-acetyldeoxynivalenol (3-ADON) (13.6%), and nivalenol (NIV) (3.5%). In F. culmorum, the prevalent genotype was 3-ADON (59.9%), while the NIV genotype accounted for the remaining 40.1%. Both, geographical and temporal patterns of trichothecene genotypes distribution were identified.