Bio: Alok Bhattacharya is an academic researcher from Tata Research Development and Design Centre. The author has contributed to research in topics: Gene & Complementation. The author has co-authored 1 publications.
TL;DR: In this article, two Escherichia coli clones expressing mycobacterium tuberculosis antigens were isolated from a gene-bank in the plasmid vector pBR 325.
Abstract: TwoEscherichia coli clones expressingMycobacterium tuberculosis antigens were isolated from a gene-bank in the plasmid vector pBR 325. ‘Western blot’ analysis revealed the presence of a unique protein band of molecular weight 68,000 and 38,000, respectively in cellextracts from each clone. The 68,000 dalton antigen was found to be expressed onEscherichia coli outer surface. Plasmid DNA from a third clone could confer leucine independence on two differentleu B mutants ofEscherichia coli but not on mutants in otherleu genes, pointing to the possibility ofgenetic complementation. Thus,Mycobacterium tuberculosis DNA is capable of expression inEscherichia coli.