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Amélie D’Attoma

Bio: Amélie D’Attoma is an academic researcher from University of Lyon. The author has contributed to research in topics: Hydrophilic interaction chromatography & Two-dimensional chromatography. The author has an hindex of 5, co-authored 5 publications receiving 222 citations.

Papers
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Journal ArticleDOI
TL;DR: A comparative study of different sets of chromatographic conditions including stationary phase, mobile phase and column temperature was carried out with mixtures of representative solutes in order to find out the best two-dimensional analytical conditions for charged compounds.

63 citations

Journal ArticleDOI
TL;DR: A procedure for RPLC×RPLC separations able to define, for a given analysis time, the optimized LC×LC parameters for achieving the best compromise between high peak capacity and low dilution is proposed.

59 citations

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TL;DR: A ten-fold gain in analysis time along with a significant gain in peak capacity are obtained with both systems compared to the most efficient one-dimensional separation of peptides recently published.

58 citations

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TL;DR: Comprehensive on-line two-dimensional liquid chromatography was used for the characterization of bio-oils obtained by fast pyrolysis of lignocellulosic biomass, and the best of both techniques was applied to the separation of the aqueous phase of a partially dehydroxygenated bio-oil.

53 citations

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TL;DR: It was found that column performance was strongly dependent on the type of stationary phase, especially in acidic medium, and ammonia acetate at neutral pH led to the best results in terms of both efficiency and peak capacity.

19 citations


Cited by
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Journal ArticleDOI
TL;DR: In this paper, a comprehensive multi-scale review of the state of the art in fast pyrolysis is presented, as well as their multiscale interactions, including the reaction mechanisms and kinetics.

288 citations

Journal ArticleDOI
TL;DR: There simply is not enough room in LC chromatograms to separate very many compounds that behave "statistically" and the attainable peak capacity does not suffice to separate complex samples, so LC cannot easily deal with complex mixtures that contain more than a few dozen analytes.
Abstract: Liquid chromatography (LC) is an incredibly successful analytical separation tool. Its versatility is unprecedented, because of the many different separation modes (reversed-phase LC, ion-exchange chromatography, size-exclusion chromatography, etc.) and because almost all samples can be dissolved in some kind of solvent, ranging from water to organic solvents to strong acids or bases. Conditions (mobile and stationary phases, additives, pH, temperatures, etc.) can be found to separate almost all pairs of analytes. For example, LC is immensely successful in the separation of enantiomers. Good selectivities can be accompanied by high efficiencies in a very short time, using contemporary ultra-high-performance liquid chromatography (UHPLC) instrumentation and (short) columns packed with sub-2-μm particles. However, LC cannot deliver very high efficiencies in a short time. Unlike other techniques, such as gas chromatography (GC) or capillary electrophoresis (CE), plate counts exceeding 100,000 are not routinely obtained in LC. As a result, LC cannot easily deal with complex mixtures that contain more than a few dozen analytes. While the selectivity between any pair of analytes can be maximized, these peaks may then start to overlap with other relevant analytes or with matrix compounds. There simply is not enough room in LC chromatograms to separate very many compounds that behave \"statistically\"1 and the attainable peak capacity does not suffice to separate complex samples. As a rule of thumb, LC offers a high probability of success for separating samples containing 10 or 20 components in 1 or 2 hours, or up to 50 components in about 10 hours2,3.

231 citations

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TL;DR: This tutorial discusses the motivations for doing two-dimensional liquid chromatography and describes the commonly used implementations of the method, and discusses the state of the art in 2D-LC performance as measured by peak capacity.
Abstract: In this tutorial, we discuss the motivations for doing two-dimensional liquid chromatography (2D-LC) and describe the commonly used implementations of the method. We review important guiding principles for method development, discuss the state of the art in 2D-LC performance as measured by peak capacity, and describe example applications from different fields that we hope will inspire new users to adopt 2D-LC for their analytical problems.

216 citations

Journal ArticleDOI
TL;DR: The rationale and principles of two‐dimensional liquid chromatography experiments are summarized, advantages and disadvantages of combining different selectivities are described, and strategies to improve the quality of two-dimensional liquid Chromatography separations are discussed.
Abstract: Online comprehensive two-dimensional liquid chromatography has become an attractive option for the analysis of complex nonvolatile samples found in various fields (e.g. environmental studies, food, life, and polymer sciences). Two-dimensional liquid chromatography complements the highly popular hyphenated systems that combine liquid chromatography with mass spectrometry. Two-dimensional liquid chromatography is also applied to the analysis of samples that are not compatible with mass spectrometry (e.g. high-molecular-weight polymers), providing important information on the distribution of the sample components along chemical dimensions (molecular weight, charge, lipophilicity, stereochemistry, etc.). Also, in comparison with conventional one-dimensional liquid chromatography, two-dimensional liquid chromatography provides a greater separation power (peak capacity). Because of the additional selectivity and higher peak capacity, the combination of two-dimensional liquid chromatography with mass spectrometry allows for simpler mixtures of compounds to be introduced in the ion source at any given time, improving quantitative analysis by reducing matrix effects. In this review, we summarize the rationale and principles of two-dimensional liquid chromatography experiments, describe advantages and disadvantages of combining different selectivities and discuss strategies to improve the quality of two-dimensional liquid chromatography separations.

167 citations