Amit Singh

Bio: Amit Singh is an academic researcher from National Institute of Technology, Patna. The author has contributed to research in topics: Digital watermarking & Watermark. The author has an hindex of 57, co-authored 640 publications receiving 13795 citations. Previous affiliations of Amit Singh include Ithaca College & Center for Infectious Disease Research and Policy.

An epithelial Nfkb2 pathway exacerbates intestinal inflammation by supplementing latent RelA dimers to the canonical NF-κB module

Abstract: Aberrant inflammation associated with human ailments, including inflammatory bowel disease (IBD), is typically fuelled by the inordinate activity of RelA/NF-κB transcription factors. As such, the canonical NF-κB module mediates controlled nuclear activation of RelA dimers from the latent cytoplasmic complexes. What provokes pathological RelA activity in the colitogenic gut remains unclear. The noncanonical NF-κB pathway promotes immune organogenesis involving Nfkb2 gene products. Because NF-κB pathways are intertwined, we asked if noncanonical signaling aggravated inflammatory RelA activity. Our investigation revealed frequent engagement of the noncanonical pathway in human IBD. In a mouse model, an Nfkb2 function exacerbated gut inflammation by amplifying the epithelial RelA activity induced upon intestinal injury. Our mechanistic studies clarified that cell-autonomous Nfkb2 signaling supplemented latent NF-κB dimers leading to hyperactive canonical RelA response in the inflamed colon. In sum, regulation of latent NF-κB dimers links noncanonical signaling to RelA-driven inflammatory pathologies and may provide for therapeutic targets. In brief Noncanonical NF-κB signals in intestinal epithelial cells supplement latent RelA dimers that, in turn, aggravated canonical NF-κB response in the colitogenic gut exacerbating intestinal inflammation. Highlights Human IBD involves the frequent engagement of the noncanonical NF-κB pathway. Mice deficient in the noncanonical signal transducer Nfkb2 are resistant to experimental colitis. Noncanonical NF-κB signaling supplements latent RelA NF-κB dimers. Noncanonical NF-κB signaling amplifies canonical NF-κB response to TLR ligands.

Barriers in implementing lean manufacturing in Indian SMEs: a multi-criteria decision-making approach

Abstract: This study aims to investigate the interrelationships among the Lean manufacturing (LM) adoption barriers in Indian SMEs. This issue has its own importance as LM has become the inescapable requirement for small- and medium-scale enterprises (SMEs) because of the increased concerns about quality, cost, delivery time and rapidly growing competition in the manufacturing sector and in India it is opposed by many factors/barriers. To act for the eradication of these barriers, we need to systematically analyze them.,Based on the available literature and consultation with the experts, the authors identified 16 LM barriers for Indian SMEs. The authors analyzed the interdependencies among the barriers and prioritized them using integrated Grey-decision-making trial and evaluation laboratory (grey-DEMATEL) approach.,The findings show that limited financial resources, fear in adopting new technology, lack of top management commitment and poor leadership quality are the most critical barriers for LM diffusion in Indian SMEs.,The present research is based on the experts’ inputs, which may be subject to individual biases. In developing countries, such as India, geographical influences are also possible, which are neglected in this study.,This study provides significant insights that can help SMEs to focus on critical cause group barriers to accelerate the LM penetration.,The authors have proposed a Grey-DEMATEL-based LM barrier evaluation framework. Here, the authors analyze the interrelationships among the barriers for LM and segregate them in cause and effect groups.

Prevalence of coronary risk factors among Indian physicians.

Abstract: BACKGROUND AND OBJECTIVES Prevalence of coronary risk factors has not been well studied in Indian physicians, therefore, to determine prevalence of selected lifestyle and biochemical coronary risk factors we performed this study. METHODS Physicians attending a national conference were invited to participate. Of the 1000 questionnaires circulated 256 physicians (221 males, 35 females) responded and were examined for presence of smoking, obesity, truncal obesity, hypertension and ECG abnormalities. Two hundred and thirty four physicians (91.4%, 203 males, 31 females) underwent a fasting blood examination for determination of glucose and lipid profile. Subjects were divided into three age groups: Group I aged or = 50 years. RESULTS Mean age of the study population was 41.7 +/- 9.3 years (range 26-70). There was no significant difference in the distribution of height, weight, body-mass index (BMI), waist and hip circumference and waist-hip ratio (WHR) in different age-groups. Systolic blood pressure (BP) and fasting glucose and triglyceride levels increased with age in both males and females, while diastolic BP, total - LDL and HDL cholesterol levels did not change. Smoking or tobacco use was seen in five males (2.3%). Prevalence of obesity (BMI > or = 25.0 kg/m2) was in 104 (48.6%) males and 18 (51.4%) females. Truncal obesity diagnosed by WHR > 0.9 in males and > 0.8 in females was in 160 (72.4%) males and 23 (65.7%) females and a large waist circumference, > or = 100 cm in males and > or = 90 cm in females, was in 58 (26.2%) males and 7 (20.0%) females. Hypertension (> or = 140/90) was in 74 (33.5%) males and 7 (20%) females. A high prevalence of diabetes diagnosed using fasting blood glucose > or = 126 mg/dl or previous history was noted in males 19 (9.4%), females 4 (12.9%). Prevalence of high total cholesterol levels > or = 200 mg/dl was in 91 (44.8%) males and 10 (32.3%) females. High LDL cholesterol level (> or = 100 mg/dl) was in 144 (70.9%) males and 22 (70.9%) females and LDL levels > or = 130 mg/dl in 70 (34.5%) males and 9 (29.0%) female physicians. High triglyceride levels (> or = 200 mg/dl) were in 38 (18.7%) males and 4 (12.9%) females. Electrocardiographic abnormalities were present in seven subjects: Q-waves in two males and one female and ST-T changes in three males and one female. BMI correlated significantly (p < 0.05) with systolic BP, fasting glucose, and triglycerides and WHR with systolic BP, diastolic BP, fasting glucose, cholesterol and triglyceride levels. CONCLUSIONS There is a high prevalence of obesity, truncal obesity, hypertension and hypercholesterolaemia in Indian physicians while smoking, low HDL cholesterol and hypertriglyceridemia is low. The overall coronary risk is lower among Indian physicians as compared to previous Indian population studies.

Cytotoxicity and Cellular Internalization Studies of Biogenic Gold Nanotriangles in Animal Cell Lines

Abstract: Biogenic gold nanotriangles have been used in this study to understand their cytotoxicity and biocompatibility in animal cells. These gold nanotriangles were synthesized using the leaf extract of the lemon grass (Cymbopogan flexuosus) plant. Cancerous as well as non-cancerous cells were used to study their dose dependent viability on exposure to the gold nanotriangles. Additionally, it has been shown that gold nanotriangles are internalized inside the cells and are compartmentalized into the cytoplasm. Thus, it has been inferred that the biologically synthesized gold nanotriangles are indeed biocompatible and, thus, are promising candidates as scaffolds for delivery of drug, genes, or growth factors inside the cells. Additionally, their unique optical properties make them a promising candidate for hyperthermic treatment of cancer. The AFM analysis of the cells treated with gold nanotriangles showed pits on their surface, which could be the probably point of entry of these nanotriangles into the c...

Rubisco Regulation in Response to Altered Carbon Status in the Cyanobacterium Synechococcus elongatus PCC 7942.

Abstract: Photosynthetic organisms possess a variety of mechanisms to achieve balance between absorbed light (source) and the capacity to metabolically utilize or dissipate this energy (sink). While regulatory processes that detect changes in metabolic status/balance are relatively well studied in plants, analogous pathways remain poorly characterized in photosynthetic microbes. Here, we explored systemic changes that result from alterations in carbon availability in the model cyanobacterium Synechococcus elongatus PCC 7942 by taking advantage of an engineered strain where influx/efflux of a central carbon metabolite, sucrose, can be regulated experimentally. We observed that induction of a high-flux sucrose export pathway leads to depletion of internal carbon storage pools (glycogen) and concurrent increases in estimates of photosynthetic activity. Further, a proteome-wide analysis and fluorescence reporter-based analysis revealed that upregulated factors following the activation of the metabolic sink are concentrated on ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) and auxiliary modules involved in Rubisco maturation. Carboxysome number and Rubisco activity also increased following engagement of sucrose secretion. Conversely, reversing the flux of sucrose by feeding exogenous sucrose through the heterologous transporter resulted in increased glycogen pools, decreased Rubisco abundance, and carboxysome reorganization. Our data suggest that Rubisco activity and organization are key variables connected to regulatory pathways involved in metabolic balancing in cyanobacteria.

疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A

Abstract: 抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1（PfPMP1）与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员，通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

Pattern Recognition and Machine Learning

Abstract: Probability Distributions.- Linear Models for Regression.- Linear Models for Classification.- Neural Networks.- Kernel Methods.- Sparse Kernel Machines.- Graphical Models.- Mixture Models and EM.- Approximate Inference.- Sampling Methods.- Continuous Latent Variables.- Sequential Data.- Combining Models.

Harrison's Principles of Internal Medicine

Abstract: The 11th edition of Harrison's Principles of Internal Medicine welcomes Anthony Fauci to its editorial staff, in addition to more than 85 new contributors. While the organization of the book is similar to previous editions, major emphasis has been placed on disorders that affect multiple organ systems. Important advances in genetics, immunology, and oncology are emphasized. Many chapters of the book have been rewritten and describe major advances in internal medicine. Subjects that received only a paragraph or two of attention in previous editions are now covered in entire chapters. Among the chapters that have been extensively revised are the chapters on infections in the compromised host, on skin rashes in infections, on many of the viral infections, including cytomegalovirus and Epstein-Barr virus, on sexually transmitted diseases, on diabetes mellitus, on disorders of bone and mineral metabolism, and on lymphadenopathy and splenomegaly. The major revisions in these chapters and many

Shape‐Controlled Synthesis of Metal Nanocrystals: Simple Chemistry Meets Complex Physics?

Abstract: Nanocrystals are fundamental to modern science and technology. Mastery over the shape of a nanocrystal enables control of its properties and enhancement of its usefulness for a given application. Our aim is to present a comprehensive review of current research activities that center on the shape-controlled synthesis of metal nanocrystals. We begin with a brief introduction to nucleation and growth within the context of metal nanocrystal synthesis, followed by a discussion of the possible shapes that a metal nanocrystal might take under different conditions. We then focus on a variety of experimental parameters that have been explored to manipulate the nucleation and growth of metal nanocrystals in solution-phase syntheses in an effort to generate specific shapes. We then elaborate on these approaches by selecting examples in which there is already reasonable understanding for the observed shape control or at least the protocols have proven to be reproducible and controllable. Finally, we highlight a number of applications that have been enabled and/or enhanced by the shape-controlled synthesis of metal nanocrystals. We conclude this article with personal perspectives on the directions toward which future research in this field might take.