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Amy C. Groth
Researcher at Stanford University
Publications - 6
Citations - 2687
Amy C. Groth is an academic researcher from Stanford University. The author has contributed to research in topics: Integrase & Recombinase. The author has an hindex of 6, co-authored 6 publications receiving 2535 citations.
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Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31.
TL;DR: The phiC31 integrase injected into embryos as mRNA functioned to promote integration of an attB-containing plasmid into the attP site, resulting in up to 55% of fertile adults producing transgenic offspring.
Journal ArticleDOI
A phage integrase directs efficient site-specific integration in human cells
TL;DR: It is demonstrated that in the presence of the phiC31 integrase, precise unidirectional integration occurs with an efficiency of 100% in Escherichia coli and >50% in human cells, forming the basis for site-specific integration strategies potentially useful in a broad range of genetic engineering applications.
Journal ArticleDOI
Phage integrases: biology and applications.
Amy C. Groth,Michele P. Calos +1 more
TL;DR: Integrases of the serine family have been shown to work efficiently in mammalian cells, mediating efficient integration at introduced att sites or native sequences that have partial identity to att sites, which has applications in areas such as gene therapy, construction of transgenic organisms, and manipulation of cell lines.
Journal ArticleDOI
Mammalian genomes contain active recombinase recognition sites.
TL;DR: It is discovered that sequences from the human and mouse genomes surprisingly divergent from loxP can support Cre-mediated recombination at up to 100% of the efficiency of the native lox P site in bacterial assays.
Journal ArticleDOI
Creating transgenic Drosophila by microinjecting the site-specific phiC31 integrase mRNA and a transgene-containing donor plasmid.
TL;DR: A microinjection-based φC31 integrase mRNA-mediated method for creating transgenic Drosophila strains is described, which is more efficient than traditional methods and ensures that the transgene is targeted to a precise genomic position.