Ana Daniela Lopes

Bio: Ana Daniela Lopes is an academic researcher from Universidade Paranaense. The author has contributed to research in topics: Genetic diversity & Biology. The author has an hindex of 9, co-authored 32 publications receiving 195 citations. Previous affiliations of Ana Daniela Lopes include Laboratory of Molecular Biology & Universidade Estadual de Maringá.

Genetic divergence in popcorn lines detected by microsatellite markers

Abstract: The purpose of this study was to use microsatellite markers to evaluate the genetic divergence in 10 popcorn lines. The number of alleles per locus ranged from 2 to 5. The proportion of polymorphic loci was highest (50%) in line Curagua, while Zelia II was the most monomorphic (100%). The arithmetic complement of similarity of Roger and Tanimoto for the 10 lines indicated lower genetic similarity between the lines Zelia II and Avati Pichinga I and higher similarity between Zelia I and Zelia II. The mean distance between groups was greatest between the group formed by lines IAC 112 I 112 IAC II, Avati Pichinga I, Avati Pichinga II and the group containing Yellow Pear Popcorn II. Strategies on how to use the heterosis between different lines in partial diallel crosses are indicated.

Magnitude of the genetic base of commercial popcorn and in recommendation in Brazil

Abstract: The purpose of this study was to quantify the genetic diversity among popcorn genotypes on the market and in the final test phase, suitable for three environments in Brazil, based on 11 agronomic traits and ISSR markers. In the environment Campos dos Goytacazes a more reliable group formation was observed than in the other environments. The molecular data indicated the presence of cultivars recommended in the same group, as in the case of UFVM2 Barao de Vicosa, IAC 125, Zelia and Jade. This result is an appeal to the few popcorn breeders in Brazil to pay particular attention to increase the genetic diversity among breeding programs conducted by public and private institutions in the country. There was virtually no correlation between the distance matrix obtained by the ISSR marker and the matrices obtained by the agronomic data.

Bioaccumulation of Lithium (Li2CO3) in Mycelia of the Culinary-Medicinal Oyster Mushroom, Pleurotus ostreatus (Agaricomycetes).

Abstract: Pleurotus ostreatus is a white-rot mushroom that bioaccumulates metals in basidiocarps and vegetative mycelia. This fungus has been used in soil and water bioremediation of several heavy metals; however, there are few studies of lithium mycelial bioaccumulation for pharmacological use. The aim of this study was to evaluate lithium bioaccumulation in P. ostreatus mycelia grown in a liquid malt extract cultivation medium with Li2CO3 or LiCl. Each lithium source was added to the medium to obtain a concentration of 0, 5, 10, 15, 20, 25, 30, 40, 50, 100, or 200 mg · L-1 lithium. The highest bioaccumulation of lithium in mycelia was 1575.29 μg · g-1 upon treatment with 40 mg · L-1 Li2CO3. P. ostreatus mycelia produce biomass and bioaccumulate both lithium sources, but more lithium bioaccumulates when in the form of Li2CO3. This study provides a prospective for the development of biotechnological products with high aggregate values and alternative ways to deliver lithium and eventually other salts for pharmacological use.

“三高人群”的Sweet Corn

Abstract: 剥开还带着田园中翠绿颜色的外衣，暴露出胎衣下那黄灿灿而饱满的果实——sweet com,甜美的玉米已经凭借其糯香的味道，从“舶来品”一跃成为了备受男人喜爱的主食之一，而它带给人们的惊喜还远远不止这些。

Efficiency of RAPD versus SSR markers for determining genetic diversity among popcorn lines.

Abstract: Using only one type of marker to quantify genetic diversity generates results that have been questioned in terms of reliability, when compared to the combined use of different markers. To compare the efficiency of the use of single versus multiple markers, we quantified genetic diversity among 10 S(7) inbred popcorn lines using both RAPD and SSR markers, and we evaluated how well these two types of markers discriminated the popcorn genotypes. These popcorn genotypes: "Yellow Pearl Popcorn" (P1-1 and P1-5), "Zelia" (P1-2 and P1-4), "Curagua" (P1-3), "IAC 112" (P9-1 and P9-2), "Avati Pichinga" (P9-3 and P9-5), and "Pisankalla" (P9-4) have different soil and climate adaptations. Using RAPD marker analysis, each primer yielded bands of variable intensities that were easily detected, as well as non-specific bands, which were discarded from the analysis. The nine primers used yielded 126 bands, of which 104 were classified as polymorphic, giving an average of 11.6 polymorphisms per primer. Using SSR procedures, the number of alleles per locus ranged from two to five, giving a total of 47 alleles for the 14 SSR loci. When comparing the groups formed using SSR and RAPD markers, there were similarities in the combinations of genotypes from the same genealogy. Correlation between genetic distances obtained through RAPD and SSR markers was relatively high (0.5453), indicating that both techniques are efficient for evaluating genetic diversity in the genotypes of popcorn that we evaluated, though RAPDs yielded more polymorphisms.

Análise biométrica de ganhos por seleção em população de milho pipoca de quinto ciclo de seleção recorrente

Abstract: Aiming to select superior progenies for concentration of favorable alleles in a popcorn population on the fifth cycle of intrapopulation full-sib recurrent selection, two hundred progenies were evaluated for six agronomic traits, including grain yield and popping expansion, in randomized complete block design with two replications within sets, in two distinct environments: Colegio Estadual Agricola Antonio Sarlo, in Campos dos Goytacazes, and Experimental Station of PESAGRO-RIO, located in Itaocara, Rio de Janeiro State, in 2007/2008. There were significant differences among families within sets in all traits, indicating the presence of genetic variability to be exploited in the future cycles. The utilized selection indexes were the Mulamba and Mock; and Smith and Hazel. The Mulamba and Mock index propitiated more elevated magnitudes of the predicted gains for most of the traits, including popping expansion and grain yield, with values of 6.01 and 8.53%, respectively, utilizing arbitrary weight attributed by tentative.