Noble metals in medicine: Latest advances

Buffers for pH and Metal Ion Control

3‐Aminopropyltriethoxysilane-Functionalized Bioanalytical Platforms for Biosensors and Diagnostics Sandeep Kumar Vashist,*,†,‡ Edmond Lam, Sabahudin Hrapovic, Keith B. Male, and John H. T. Luong †HSG-IMIT Institut für Mikround Informationstechnik, Georges-Koehler-Allee 103, 79110 Freiburg, Germany ‡Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany National Research Council Canada, Montreal, Quebec H4P 2R2, Canada Innovative Chromatography Group, Irish Separation Science Cluster (ISSC), Department of Chemistry and Analytical, Biological Chemistry Research Facility (ABCRF), University College Cork, Cork, Ireland

/pdf/immobilization-of-antibodies-and-enzymes-on-3-3um0ob31sw.pdf

Immobilization of antibodies and enzymes on 3-aminopropyltriethoxysilane-functionalized bioanalytical platforms for biosensors and diagnostics.

DNA has played an early and powerful role in the development of bottom-up nanotechnologies, not least because of DNA's precise, predictable, and controllable properties of assembly on the nanometer scale. Watson-Crick complementarity has been used to build complex 2D and 3D architectures and design a number of nanometer-scale systems for molecular computing, transport, motors, and biosensing applications. Most of such devices are built with classical B-DNA helices and involve classical A-T/U and G-C base pairs. However, in addition to the above components underlying the iconic double helix, a number of alternative pairing schemes of nucleobases are known. This review focuses on two of these noncanonical classes of DNA helices: G-quadruplexes and the i-motif. The unique properties of these two classes of DNA helix have been utilized toward some remarkable constructions and applications: G-wires; nanostructures such as DNA origami; reconfigurable structures and nanodevices; the formation and utilization of hemin-utilizing DNAzymes, capable of generating varied outputs from biosensing nanostructures; composite nanostructures made up of DNA as well as inorganic materials; and the construction of nanocarriers that show promise for the therapeutics of diseases.

DNA Quadruple Helices in Nanotechnology.

https://repositorium.sdum.uminho.pt/bitstream/1822/20589/1/file.pdf

Dendrimers and derivatives as a potential therapeutic tool in regenerative medicine strategies—A review

http://www1.ci.uc.pt/pessoal/anabrett/pdfs/PDF-2009/133-BDD-EleCom.pdf

Hydroxyl radicals electrochemically generated in situ on a boron-doped diamond electrode

As carrier of genetic information, DNA is one of the most important intracellular targets that undergo modification and damage upon interaction with endogenous and exogenous factors. DNA is an excellent biomaterial for the construction of new devices, in nanotechnology and biosensor technology, for evaluation of DNA interaction with a broad range of chemical compounds and biomolecules, essential from a biological and a medical point of view. This review discusses recent advances on the design and applications of DNA-electrochemical biosensors that use DNA direct electrochemistry as a detection platform. AFM and voltammetric characterization of new bottom up immobilisation procedures of self-assembled nanostructures based on DNA single- and double-stranded, G-quadruplex, and i-motif configurations are presented, relevant for the development of new DNA-electrochemical biosensor devices. The applications of DNA-electrochemical biosensors, for the label-free detection of interactions with proteins, pharmaceutical compounds, metal ions and metal complexes, pollutants, free radicals, and electromagnetic radiation, were revisited.

Applications of a DNA-electrochemical biosensor

Natural phenolic compounds are abundant in the vegetable kingdom, occurring mainly as secondary metabolites in a wide variety of chemical structures Around 10,000 different plant phenolic derivatives have been isolated and identified This review provides an exhaustive overview concerning the electron transfer reactions in natural polyphenols, from the point of view of their in vitro antioxidant and/or pro-oxidant mode of action, as well as their identification in highly complex matrixes, for example, fruits, vegetables, wine, food supplements, relevant for food quality control, nutrition, and health research The accurate assessment of polyphenols' redox behavior is essential, and the application of the electrochemical methods in routine quality control of natural products and foods, where the polyphenols antioxidant activity needs to be quantified in vitro, is of the utmost importance The phenol moiety oxidation pathways and the effect of substituents and experimental conditions on their electrochemical behavior will be reviewed The fundamental principles concerning the redox behavior of natural polyphenols, specifically flavonoids and other benzopyran derivatives, phenolic acids and ester derivatives, quinones, lignins, tannins, lignans, essential oils, stilbenes, curcuminoids, and chalcones, will be described The final sections will focus on the electroanalysis of phenolic antioxidants in natural products and the electroanalytical evaluation of in vitro total antioxidant capacity

https://onlinelibrary.wiley.com/doi/pdf/10.1111/1541-4337.12566

Natural phenolic antioxidants electrochemistry: Towards a new food science methodology

Palladium nanoparticles and nanowires electrochemically deposited onto a carbon surface were studied using cyclic voltammetry, impedance spectroscopy and atomic force microscopy. The ex situ and in situ atomic force microscopy (AFM) topographic images showed that nanoparticles and nanowires of palladium were preferentially electrodeposited to surface defects on the highly oriented pyrolytic graphite surface and enabled the determination of the Pd nanostructure dimensions on the order of 50–150 nm. The palladium nanoparticles and nanowires electrochemically deposited onto a glassy carbon surface behave differently with respect to the pH of the electrolyte buffer solution. In acid or mild acid solutions under applied negative potential, hydrogen can be adsorbed/absorbed onto/into the palladium lattice. By controlling the applied negative potential, different quantities of hydrogen can be incorporated, and this process was followed, analysing the oxidation peak of hydrogen. It is also shown that the growth of the Pd oxide layer begins at negative potentials with the formation of a pre-monolayer oxide film, at a potential well before the hydrogen evolution region. At positive potentials, Pd(0) nanoparticles undergo oxidation, and the formation of a mixed oxide layer was observed, which can act as nucleation points for Pd metal growth, increasing the metal electrode surface coverage. Depending on thickness and composition, this oxide layer can be reversibly reduced. AFM images confirmed that the PdO and PdO2 oxides formed on the surface may act as nucleation points for Pd metal growth, increasing the metal electrode surface coverage.

/pdf/palladium-nanoparticles-and-nanowires-deposited-25szwgdcga.pdf

Palladium nanoparticles and nanowires deposited electrochemically: AFM and electrochemical characterization

http://www1.ci.uc.pt/pessoal/anabrett/pdfs/PDF-2009/131-Talidomida-Bioelectrochemistry.pdf

Ana-Maria Chiorcea-Paquim

Papers

Hydroxyl radicals electrochemically generated in situ on a boron-doped diamond electrode

Applications of a DNA-electrochemical biosensor

Natural phenolic antioxidants electrochemistry: Towards a new food science methodology

Palladium nanoparticles and nanowires deposited electrochemically: AFM and electrochemical characterization

In situ electrochemical and AFM study of thalidomide-DNA interaction