Comparative analysis of liver transcriptomes associated with hypoxia tolerance in the gynogenetic blunt snout bream

Gynogenetic diploid was induced in red crucian carp (RCC) (Carassius auratus Red Variety) eggs using UV-irradiated spermatozoa from blunt snout bream (B) (Megalabrama amblycephala) or from mirror carp (C) (Cyprinus carpio. L). Spermatozoa were genetically inactivated by an appropriate UV dosage, and then the maternal DNA was duplicated with cold shock at 0–4°C. When using the spermatozoa of B, the fertilization rate, hatching rate and survival at first feeding were 52.6 ± 3.0 %, 23.6 ± 4.1 % and 15.7 ± 3.4 % respectively, and the survival at first feeding was significantly higher than that (11.3 ± 2.2%) when using the spermatozoa of C(Cypripnus carpio. L). According to the morphological characteristics, the chromosome number and the degree of gonadal development, gynogenetic RCC could be distinguished from the control hybrids of RCC ♀ × B♂. The individuals with red body color, 100 chromosomes and normal gonadal development were syccessful gyogenetic RCC, while the individuals with 124 or 148 chro...

Induction of gynogenesis in red crucian carp using Spermatozoa of blunt snout bream

The first case of induced gynogenesis in Neotropical fishes using the yellowtail tetra (Astyanax altiparanae) as a model organism

Telophase of the first cleavage is the key stage for optimally inducing mitotic gynogenesis in rice field eel (Monopterus albus)

Comparative study on growth performance and morphological characteristics of the meio- and mito-gynogenesis olive flounder (Paralichthys olivaceus)

Induction of diploid gynogenesis by heat shock treatment in silver barb (Barbonymus gonionotus)

The experiment was conducted to compare the growth performance of gynogenetic females and gynogenetic neo-males with mixed-sex silver barb (Barbonymus gonionotus) in six earthen ponds for a period of 120 days. Three treatments i.e. T1 (Gynogenetic female), T2 (Gynogenetic neo-male) and T3 (mixed-sex, control) each having two replicates were stocked with 60 fish/decimal. During stocking the mean length and weight of fishes were 5.83±0.12 cm and 2.72±0.10 g, 5.85±0.12 cm and 2.77±0.12 g and 5.79±0.16 cm and 2.66±0.16 g, in T1, T2 and T3, respectively. The fishes were reared with a commercial fish feed (Mega Feed) of different types such as pre-starter (36% crude protein) for the first 42 days, starter (32% protein) for the second 42 days and grower (32% protein) for the last 36 days. Water quality parameters were measured and found in optimal levels for fish culture. A significantly highest (p 0.05) different. Similar trend was also observed in other growth parameters such as final length, final weight, % weight gain, average daily gain and specific growth rate among the treatments. However, no significant difference (p>0.05) was observed for survival among the treatments. The significantly highest (p>0.05) gross and net production was observed in T1 (1219.62±23.29 and 1178.83±22.33 kg/ha/120 days) and lowest in T3 (1029.38±22.44 and 989.54±21.30 kg/ha/120 days) and no significantly different (p> 0.05) was observed for the production between T1 and T2. Considering the growth performance of silver barb, it can be concluded that the gynogenetic females and neo-males possess higher production potentiality and thus generation of all-female population using neo-males would be an effective approach for accelerating fish production.

/pdf/comparison-of-growth-performance-of-gynogenetic-female-4mw1tbap56.pdf

Comparison of growth performance of gynogenetic female, gynogenetic neo-male and normal mixed-sex silver barb (Barbonymus gonionotus) in earthen ponds

This study was dealt with induction of diploid meiotic and mitotic gynogenesis in Barbonymus gonionotus through cold shock treatment for production of monosex all-female population. For inhibition of paternal inheritance, sperm DNA was denatured by exposing cell suspension containing 8×108 ml-1 to UV at 196 μWcm-2 for 1.5 min. After fertilization of eggs using uv-treated sperm, meiotic diploidization was done by cold shock treatment at 2 °C for 1 min applied 1.5 min of post-fertilization that produced 44.23±0.62% meiotic gynogens. Mitotic diploidization was done by the same cold shock treatment applied after 27.5 min of fertilization that produced 36.70±0.63% mitotic gynogens. Ploidy status of all the progenies was evaluated by karyotyping. The karyotype analysis revealed 23 (N) chromosomes in haploids, and 46 (2N) chromosomes in meiotic and mitotic gynogens and controls. Sexing of fish showed nearly all-female, ranging from 98.28 to 100% and 96.43 to 100% female in the meiotic and mitotic gynogens respectively. The sex ratio in the control group was nearly 1:1. DNA microsatellite analysis revealed that the meiotic gynogenetic offspring possessed two alleles the same as their mother and the mitotic gynogenetic offspring possessed any of the two alleles of their mother and became homozygous. No meiotic and mitotic gynogens have inheritance of paternal alleles. The control offspring shared alleles from both male and female parents. Thus, the study confirmed the production of meiotic and mitotic gynogens of silver barb through cold shock treatment and they could be converted into sex-reversed males (XX) by masculinizing hormone treatment for production of monosex all-female population.

Ananaya A. Nishat

Papers

Induction of diploid gynogenesis by heat shock treatment in silver barb (Barbonymus gonionotus)

Comparison of growth performance of gynogenetic female, gynogenetic neo-male and normal mixed-sex silver barb (Barbonymus gonionotus) in earthen ponds

Induction of meiotic and mitotic gynogenesis in silver barb (Barbonymus gonionotus) through cold shock treatment