Andaru Satryo

Bio: Andaru Satryo is an academic researcher from Gadjah Mada University. The author has contributed to research in topics: Agrostophyllum & Dendrobium. The author has an hindex of 1, co-authored 2 publications receiving 3 citations.

CYTOTOXIC EFFECT OF AQUADEST, ETHANOLIC, AND CHLOROFORM EXTRACTS OF Spathoglottis plicata Blume ON BREAST CANCER CELLS LINE (T47D CELLS)

Abstract: Breast cancer is one of cancer with high mortality. This cancer not only attacks women, but also men. Indonesia has many plants which potential as anticancer, such as orchids. Spathoglottis plicata is one of the orchid species that abundant in Indonesia and has a lot of antioxidant compounds which is guessed have anticancer properties. The objectives of this study were to study the cytotoxic activity and IC 50 value of aquadest, ethanolic, and chloroform extracts of S. plicata ’s pseudobulbs, leaves, and whole plants on T47D cells (breast cancer cells line) as well as cytotoxic activity of the specific fraction of the most toxic crude extract. S. plicata used in this study was obtained from Bungarinte nursery. Extractions were done by maceration method using aquadest, ethanol, and chloroform as the solvent. Cytotoxic test on T47D cells were done by MTT assay. The cytotoxic data were analyzed using one-way ANOVA followed by Tukey’s HSD test. The IC 50 of each extracts were calculate by probit analysis. The lowest IC 50 value among all extracts was fractionated and isolated by preparative TLC. The cytotoxic activity and IC 50 of this fractions were analyzed. The results showed that only 2 from 9 crude extracts that able to calculate its IC 50 because those two extracts have concentration dependent pattern of inhibition concentration. Chloroform extract have the lowest IC 50 value (369,837 μg/mL). Then, this extract fractionated by eluen n-hexane : ethyl acetate 4:1. Four fractions were collected. The lowest IC 50 value is fraction IV (144,41 μg/mL). Based on the results it could be concluded that S. plicata leaves have moderate potency to develop as anticancet agents, especially on breast cancer. Keywords : S. plicata , T47D cells, cytotoxic , MTT assay, preparative TLC .

Species diversity of epiphytic orchids in natural reserve of Mount Sibela, North Moluccas

Abstract: Sibela Mount Natural Reserve is located in South Halmahera, North Moluccas, Indonesia. It covers a 23 232 ha area of coastal forest, lowland rain forest and highland rain forest. The altitude range between 0 m to 2 110 m. above sea level (asl). Orchid exploration and inventory were conducted to study orchid diversity and distribution on this conservation areas. Observation on the population of epiphyte orchids was also done to study the dominant orchid in that area. It was recorded that there were 30 orchid species belonging to 14 genera (i.e. Aerides, Agrostophyllum, Bulbophyllum, Coelogyne, Dendrobium, Diplocaulobium, Eria, Flickingeria, Luisia, Pomatocalpa, Phreatia, Thelasis, Trichoglottis and Vandopsis) which were genera is the member of Epidendroideae subfamily. The genus with the highest frequency (44.59 %) was Dendrobium. Regarding elevation, the highest distribution of the epiphytic orchids in coastal forest and lowland rain forest ecosystem at 0 m to 600 m. asl.

An illustrated survey of orchid genera.

Abstract: More than 150 orchid genera are described, each accompanied by a superb Marion Sheehan illustration that shows a representative species; all display the same structures in similar views so that the reader can easily compare the variations among genera. In 1995, the authors received the American Orchid Society's Gold Medal of Achievement.

Orchid conservation in a small island: current study and challenges of Dendrobium striaenopsis conservation in Angwarmase island nature reserve, Moluccas, Indonesia

Abstract: Orchids are considered one of the most commercially valuable in ornamental plant. Dendrobium striaenopsis, an endemic orchid to Tanimbar Islands, Moluccas, is widely notable species for orchid hybridizing. However, with its restricted distribution to small islands, this species is prone to extinction. To date, the ecological aspects of D. striaenopsis are not well studied, thus can hamper its conservation efforts. 30 plots in 3 different types of habitat in Angwarmase Island Nature Reserve were surveyed to assess its abundance and habitat. The locals surrounding the reserve were interviewed to identify potential threats. 165 individuals D. striaenopsis were found during survey. The result indicated that D. striaenopsis was not host-specific orchid species. The species was found in 15 species host trees with Lumnitzera racemose was the common host species. Zone 2 (upper part of the main stem) was revealed as the most favorable part at the host tree (105 individuals). It was found that the habitat of the species was threatened by agriculture activities. Besides, D. striaenopsis was also traded illegally by locals.

Validity of BI-RADS System Mammography to detect breast cancer at Dr Sardjito Hospital Yogyakarta

Abstract: Background: Breast cancer is the second most common cancer among women worldwide and the sixth common cause of mortality in Indonesia. The diagnosis of breast cancer may be established by physical and imaging examinations, but definitive diagnosis should be verified by histopathologic examination. Mammography is an easy non-invasive imaging method to detect breast cancer with good accuracy. The lack of uniformity in mammography reporting has led to confusion as to the interpretation and management recommendation. The American College of Radiology Breast Imaging Reporting and Data System (BIRADS) has provided a standardized reporting system for mammography. The validity of this system at Dr Sardjito Hospital Yogyakarta has not been evaluated. Objective: To know the sensitivity, specificity, positive and negative predictive values, and accuracy of the BI-RADS System Mammography in diagnosing breast cancer. Methods: This study was a diagnostic test with cross sectional design on breast cancer diagnosis based on BI-RADS system mammography. All medical record files of patients with breast lump who underwent mammography and histopatologic examination, at Dr Sardjito Hospital Yogyakarta between December 2003-March 2008, were included in the analysis. Results: BI-RADS System Mammography at Dr Sardjito Hospital Yogyakarta had sensitivity of 87.5%, specificity of 93.7%, positive predictive value of 96.5%, negative predictive value 78.95%, positive likelihood ratio 14, negative likelihood ratio 0.13, prevalence 66.6%, and accuracy 89.6%. Conclusion: Mammography examination using BI-RADS System in Sardjito Hospital Yogyakarta was favourable in detecting breastcancer. Key words: breast cancer - BI-RADS mammography - histopathology - diagnostic value