Showing papers by "Anders Björklund published in 1976"
TL;DR: While the majority of the monoamine-containing neurons in the transplants died within the first month after transplantation, a significant number of neurons survived for at least half a year in the brain.
484 citations
TL;DR: Transplants of adrenergic, dopaminergic or indolaminergic neurones were transplanted to a cavity in the retrosplenial cortex to give them the opportunity to grow along the lesioned temporoammonic perforant path into the partly denervated hippocampal formation, demonstrating a remarkable ability of the regenerating axons to establish reproducible and characteristic terminal patterns in large areas of the hippocampus.
Abstract: CENTRAL and peripheral monoaminergic neurones have been found to survive transplantation to certain sites in the adult rat brain1. The neurones grow extensively in their new location and the newly formed axons extend through the surrounding scar tissue into the host brain. We now report experiments in which transplants of adrenergic, dopaminergic or indolaminergic neurones were transplanted to a cavity in the retrosplenial cortex to give them the opportunity to grow along the lesioned temporoammonic perforant path into the partly denervated hippocampal formation. The results demonstrate a remarkable ability of the regenerating axons to establish reproducible and characteristic terminal patterns in large areas of the hippocampus.
252 citations
TL;DR: The present technique has a sensitivity for the dopamine- and noradrenaline-containing systems that is comparable with that of the glyoxylic acid-Vibratome technique, which utilizes fresh, glyoxy Lic acid-perfused tissue.
Abstract: A technique is described for highly sensitive and precise visualization of central catecholamine systems in paraffin sections of freeze-dried tissue. The procedure is based on perfusion of the animal with a solution containing formaldehyde and/or glyoxylic acid, in the presence of a very high magnesium content (40 g MgSO4/150 ml solution) and acid pH. The perfused tissue is rapidly frozen, freeze-dried, treated with formaldehyde vapours (at +80°C for 1h), embedded in paraffin in vacuo, and finally sectioned.
129 citations
TL;DR: The regrowth of lesioned central acetylcholinesterase (AChE)-positive axons in the adult rat was studied in irides implanted to two different brain sites, and central cholinergic neurones seem partly able to replace the peripheral ones in the reinnervation of a denervated peripheral target.
75 citations
TL;DR: The ability of the iris to support regenerative ingrowth by fibres not entirely appropriate for normal re-innervation suggested to us that the iri might also support regeneratives ingrowthBy fibres containing putative amino acid transmitters.
Abstract: IRIDES transplanted into the central nervous system (CNS) of the rat provide a good substrate for regenerative ingrowth of central noradrenergic and cholinergic fibres to ‘reinnervate’ the denervated iris1–3. Fluorescence histo-chemistry or acetylcholinesterase staining reveals that the regenerating noradrenergic or cholinergic fibres will produce an innervation that closely mimics the original noradrenergic or cholinergic innervation1–3. Physiological experiments4 on portal vein transplants indicate that functional adrenergic and cholinergic terminals are formed, and it seems likely that functional neuromuscular junctions are also re-established in the iris transplants. In addition to supporting the ingrowth of noradrenergic and cholinergic fibres, fluorescence histochemistry2 reveals that 5-hydroxy-tryptamine- and dopamine-containing fibres will also grow into iris transplants, albeit less extensively than noradrenergic fibres. This ability of the iris to support regenerative ingrowth by fibres not entirely appropriate for normal re-innervation suggested to us that the iris might also support regenerative ingrowth by fibres containing putative amino acid transmitters.
19 citations
01 Jan 1976
TL;DR: The morphological alterations induced by the anti-NGF serum treatment in the adrenergic terminals, and the time course of these changes, strongly suggest that besides a reduction in neuronal NA content, the antiserum treatment causes an actual degeneration of nerve terminals.
Abstract: Publisher Summary This chapter discusses the role of nerve growth factor (NGF) in maintenance and regeneration of adrenergic axons. There is considerable evidence that nerve growth factor plays an important role in the ontogenetic growth and the development of sympathetic adrenergic neurons. The morphological alterations induced by the anti-NGF serum treatment in the adrenergic terminals, and the time course of these changes, strongly suggest that besides a reduction in neuronal NA content, the antiserum treatment causes an actual degeneration of nerve terminals. There is much to indicate that the NGF-sensitivity of adrenergic neurons changes quantitatively and also qualitatively during the first few weeks of life, which coincides with the time of maturation of the sympathetic nervous system in the mouse. Whereas in the chick embryo and the newborn mouse, NGF has been reported to induce an increase both in size and number of adrenergic ganglion cell bodies, there is probably only a hypertrophic response of the adrenergic cell bodies in the adult. In most of the peripheral tissues, innervated by both para- and prevertebral sympathetic chain ganglia, the NGF treatment caused a marked stimulation of the axonal regeneration, as observed at both 9 and 21 days after the 6-OH-DA induced axonal damage.
5 citations