Andrea Illingworth

TL;DR: Flow cytometry is the method of choice for identifying cells deficient in GPI‐linked proteins and is, therefore, necessary for the diagnosis of PNH, but to date there has not been an attempt to standardize the methodology used to identify these cells.

TL;DR: Paroxysmal nocturnal hemoglobinuria is a life‐threatening disorder caused by an inability to make glyco‐phosphatidyl‐inositol (GPI) anchors and flow cytometry is the method of choice to detect the loss of GPI‐linked proteins.

TL;DR: The goal is to provide flow cytometry laboratories with an updated consensus approach to analysis and reporting of PNH results and to address the most common analytical challenges for accurate reporting of this rare disease.

TL;DR: The EXPLORE study aimed to determine the prevalence of undiagnosed PNH in patients with aplastic anemia, myelodysplastic syndrome, and/or other bone marrow failure (BMF) syndromes and the effect of PNH clone size on hemolysis.

TL;DR: This section (Part 2) of the updated ICCS PNH Consensus Guidelines will focus on specific instrument setup for these PNH assays, the identification and proper testing of appropriate antibody conjugates and combinations therof, and basic assay design.