Annytha Ina Rohi Detha

Bio: Annytha Ina Rohi Detha is an academic researcher from University of Nusa Cendana. The author has contributed to research in topics: Lactic acid & Antimicrobial. The author has an hindex of 5, co-authored 26 publications receiving 66 citations.

Karakteristik Antimikroba Bakteri Asam Laktat Susu Kuda Sumba Terhadap Bakteri Salmonella Typhimurium

Abstract: Tujuan dari penelitian ini adalah untuk mengidentifikasi aktivitas antimikroba dari bakteri asam laktat yang diisolasi dari susu sumba terhadap bakteri patogen Salmonella Typhimurium; dan membandingkan penghambatan bakteri asam laktat dalam bentuk filtrat dan non-filtrat. Penelitian ini dilakukan di Laboratorium Penyakit Hewan dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Universitas Nusa Cendana. Pembuatan Filtrat Bakteri Asam Laktat dan Bakteri non-Filtrat dari asam laktat utuh adalah bakteri asam laktat yang non-filtrat, sedangkan filtrat bakteri asam laktat melewati proses centrifuge dari MRS Broth dan dituangkan ke dalam tabung reaksi dan disentrifugasi pada 3000 rpm untuk 30 menit untuk memisahkan supernatan dari endapan. Berdasarkan data, diameter zona hambat filtrat bakteri asam laktat dalam metode sumur menunjukkan hasil 12,2±2,1 mm Diameter zona hambat bakteri asam laktat non-filtrat dalam metode sumur menunjukkan hasil 13,3±0,1 mm. Berdasarkan perbandingan, bakteri asam laktat menggunakan metode sumur non filtrat memiliki zona hambat yang lebih rendah daripada metode sumur non filtrat. Bakteri asam laktat memiliki kategori kekuatan antimikroba yang kuat terhadap bakteri Salmonella Typhimurium dan diameter zona hambat optimal bakteri asam laktat dalam bentuk non-filtrat lebih besar daripada diameter zona hambat optimal bakteri asam laktat dalam bentuk filtrat.

Antimicrobial Properties of Lauric Acid and Monolaurin in Virgin Coconut Oil: A Review

Abstract: Virgin coconut oil is obtained by wet processing of coconut milk using fermentation, centrifugation, enzymatic extraction, and the microwave heating method. Presently, VCO has several positive effects and benefits to human health, hence, it is regularly consumed and widely known as a unique functional food. VCO contains lauric acid (45 to 52 %). By lipase in the digestive system, VCO can undergo a breakdown into lauric acid, 1-monolaurin, and 2-monolaurin. These components have both hydrophilic and lipophilic groups and are also recognized as excellent antimicrobial lipids. Furthermore, lauric acid and monolaurin can be used as antibacterial, antifungal, and antiviral with broad-spectrum inhibition. Lauric acid and monolaurin have a strong ability to destroy gram-positive bacteria, especially S. aureus, fungi such as C. Albicans, and viruses including vesicular stomatitis virus (VSV), herpes simplex virus (HSV), and visna virus (VV). Lauric acid and monolaurin interact with certain functional groups located in the cell membrane and can cause damage to the cell. In general, the potential of VCO as healthy food is contributed by lauric acid and monolaurin which are antimicrobial agents.

Seroprevalence of Japanese encephalitis virus using competitive enzyme linked immunosorbent assay (C-ELISA) in pigs in East Sumba, Indonesia

Abstract: Japanese Encephalitis (JE), a vector-borne zoonotic viral disease, is mostly prevalent in Asian countries. The objective of this study was to investigate the occurence of JE virus (JEV) among pigs in East Sumba, Indonesia. Blood samples (n=52) were randomly collected from 52 apparantly healthy pigs where pig population was high in East Sumba. The samples were subjected for seroprevalence study for the presence of antibodies against JEV using competitive enzyme linked immunosorbent assay (C-ELISA). Results showed that 53% (n=28/52) blood samples from the pigs contained antibodies against JEV. This finding is suggestive that the JEV is circulating among pig population in East Sumba, Indonesia. The data may help in designing control strategies of the JEV in the East Sumba, Indonesia.

Antimicrobial activity of traditional wines (Sopi and Moke) against Salmonella sp. and Escherichia coli

Abstract: Objective: Sopi and Moke are two traditional wines in Indonesia. The present study aimed at investigating the antimicrobial effects of Sopi and Moke as compared to other commercial disinfectants. Materials and methods: The alcohol level and pH of the traditional wines ( Sopi and Moke ) were determined by alcohol meter and pH meter, respectively. The susceptibility test was perfomed to determine the antimicrobial activity of Sopi against E scherichia c oli which was isolated from cattle, and the activity of Moke was tested against Salmonella sp . which was a local isolate of poultry. Results: In susceptibility test, Sopi showed 17.5 mm in zone of inhibition against E. coli , while Formades ® , a commercial disinfectant showed 16 mm of zone of inhibition against the same bacteria. Moke showed 17.5 mm inhibition zone against Salmonella sp . , whereas Antisep ® , a commercial disinfectant had 28 mm of inhibition zone against the same isolate. Conclusion: The results indicate that Sopi and Moke have antimicrobial effects on E. coli and Salmonella sp. , respectively. The findings of this study suggest that Sopi and Moke can be used as potential antimicrobial agents. http://doi.org/10.5455/javar.2016.c166

Fractionation and Identification Antimicrobial Activity of Sumba Mare Milk Protein Against Causative Agent of Subclinical Mastitis

Abstract: The aims of this study were to fractionate and to identify antimicrobial activity of Sumba mare's milk protein against causative agent of subclinical mastitis. Antimicrobial compounds isolation of whey protein were fractionate using alkaline alumina column. Determination of antimicrobial activity of protein fractions through suseptibility test against bacteria causing subclinical mastitis which have been isolated and confirmed (sugar fermentation, indole, methyl red, voges proskauer, hydrogen sulphide, citrate and catalase) from dairy farms in the area of Bogor. Identification of antimicrobial compounds using HPLC method, there are six main peaks conferring to their different polarities and retention times. Fractionation results of six fractions with different polarity levels were tested for antimicrobial activity against bacteria causing subclinical mastitis (Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae and Streptococcus pyogenes). The third whey protein fraction, soluble in acetone exhibited significant inhibition activity on the growth of S. agalactiae and S. pyogenes.

How Central Is the Domestic Pig in the Epidemiological Cycle of Japanese Encephalitis Virus? A Review of Scientific Evidence and Implications for Disease Control.

Abstract: Despite the existence of human vaccines, Japanese encephalitis (JE) remains the leading cause of human encephalitis in Asia. Pigs are described as the main amplifying host, but their role in JE epidemiology needs to be reassessed in order to identify and implement efficient control strategies, for both human and animal health. We aimed to provide a systematic review of publications linked to JE in swine, in terms of both individual and population characteristics of JE virus (JEV) infection and circulation, as well as observed epidemiological patterns. We used the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement to select and analyze relevant articles from the Scopus database, 127 of which were included in the review. Pigs are central, but the implication of secondary hosts cannot be ruled out and should be further investigated. Although human vaccination cannot eradicate the virus, it is clearly the most important means of preventing human disease. However, a better understanding of the actual involvement of domestic pigs as well as other potential JEV hosts in different JEV epidemiological cycles and patterns could help to identify additional/complementary control measures, either by targeting pigs or not, and in some specific epidemiological contexts, contribute to reduce virus circulation and protect humans from JEV infection.

Bioactivities and analysis of peptides from Sumbawa horse milk generated by Bacillus thuringiensis protease

Abstract: Sumbawa horse milk is claimed to cure some diseases such as asthma, hypertension, diabetes and gastrointestinal disorder but its potential bioactive peptide has not been explored. The aims of this study are to evaluate bioactivities peptides from Sumbawa horse milk protein hydrolysate and to analyzethe physio-chemical properties of selected peptides. The milk protein was hydrolyzed by Bacillus thuringiensis protease, the peptide produced were sequential fractionated and then analyzed for antibacterial and antioxidant activities. The peptide fraction <3 kDa was then sequenced using LCMS-MS and the physio-chemical properties of the peptides were analyzed. The result showed that peptides fraction <3 kDa from the 30 min hydrolysis was the most active as antibacterial and more active to Gram negative bacteria. For antioxidant, scavenging activity of the fraction per µg protein/mL were 83% to ABTS and 31% to DPPH radicals. The values were similar with vitamin C 12.5 µg/mL for ABTS and 14.5 µg/mL for DPPH. Peptide HPYFYAPELLYYANK with molecular weight prediction 1887.92 Da and isoelectric point 7.47 has high therapeutic index prediction (64.75). The result showed that peptides from Sumbawa horse milk hydrolyzed by Bacillus thuringiensis protease was active as antibacterial and antioxidant. Peptide HPYFYAPELLYYANK from fraction <3 kDa was potential as antibacterial.

Karakteristik Antimikroba Bakteri Asam Laktat Susu Kuda Sumba Terhadap Bakteri Salmonella Typhimurium

Abstract: Tujuan dari penelitian ini adalah untuk mengidentifikasi aktivitas antimikroba dari bakteri asam laktat yang diisolasi dari susu sumba terhadap bakteri patogen Salmonella Typhimurium; dan membandingkan penghambatan bakteri asam laktat dalam bentuk filtrat dan non-filtrat. Penelitian ini dilakukan di Laboratorium Penyakit Hewan dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Universitas Nusa Cendana. Pembuatan Filtrat Bakteri Asam Laktat dan Bakteri non-Filtrat dari asam laktat utuh adalah bakteri asam laktat yang non-filtrat, sedangkan filtrat bakteri asam laktat melewati proses centrifuge dari MRS Broth dan dituangkan ke dalam tabung reaksi dan disentrifugasi pada 3000 rpm untuk 30 menit untuk memisahkan supernatan dari endapan. Berdasarkan data, diameter zona hambat filtrat bakteri asam laktat dalam metode sumur menunjukkan hasil 12,2±2,1 mm Diameter zona hambat bakteri asam laktat non-filtrat dalam metode sumur menunjukkan hasil 13,3±0,1 mm. Berdasarkan perbandingan, bakteri asam laktat menggunakan metode sumur non filtrat memiliki zona hambat yang lebih rendah daripada metode sumur non filtrat. Bakteri asam laktat memiliki kategori kekuatan antimikroba yang kuat terhadap bakteri Salmonella Typhimurium dan diameter zona hambat optimal bakteri asam laktat dalam bentuk non-filtrat lebih besar daripada diameter zona hambat optimal bakteri asam laktat dalam bentuk filtrat.

Anisakiasis Pada Ikan Laut Di Indonesia: Prevalensi, Sebaran Dan Potensi Patogenitasnya Pada Manusia

Abstract: Nematode worm Anisakis sp is the causative agent of anisakiasis, a zoonosis which continues to be a health problem in humans, globally. Its clinical spectum varies from mild to very severe and always connected with sea fishes, one important source of nutrition for the community that cannot be ignored. In addition, consumer’s behavior and lifestyle in processing and consuming sea fishes also have has the potency as the predisposing factor for its transmission and clinical manifestations. This review article aimed to describes recent global data on anisakiasis and its occurrence in marine fishes of Indonesian waters and the risk of transmission in the community.

Karakterisasi parasit pada komoditas perikanan di Balai Karantina Ikan, Pengendalian Mutu, dan Keamanan Hasil Perikanan Semarang, Jawa Tengah.

Abstract: Parasite is one of the infectious disease pathogens in fish which can cause secondary infection by bacteria, fungi and viruses. Therefore, fish quarantine activities are needed to prevent disease transmission and are carried out by the fish quarantine center. This study aimed to characterize the types of parasites that infect fish commodities that will be trafficked at the Fish Quarantine Center, Quality Control and Safety of Fishery Products in Semarang, Central Java. Parasitic examination was carried out using the original microscopic method by scraping the mucus, gills and internal organs, then characterizing it morphologically. The results showed that the infected parasites in fish samples included ectoparasites (Trichodina, Gyrodactylus, Dactylogyrus, Chilodonella, and Argulus) and endoparasites (Anisakis). Keywords: Parasites Fishery Commodity BKIPMHP Semarang ABSTRAK Parasit merupakan salah satu patogen penyebab penyakit infeksius yang dapat memicu terjadinya infeksi sekunder oleh bakteri, jamur maupun virus. Oleh sebab itu, perlu dilakukan tindakan karantina ikan untuk mencegah terjadinya penyebaran penyakit yang dilakukan oleh balai karantina ikan. Penelitian ini bertujuan untuk mengkarakterisasi jenis parasit yang menginfeksi komoditas ikan yang akan dilalulintaskan di Balai Karantina Ikan, Pengendalian Mutu dan Keamanan Hasil Perikanan Semarang, Jawa Tengah. Metode penelitian yang digunakan yaitu pemeriksaan secara mikroskopis menggunakan metode natif/langsung pada lendir, insang dan organ dalam ikan, dilakukan karakterisasi secara morfologis. Hasil penelitian menunjukkan bahwa sampel ikan terinfeksi parasit dari golongan ektoparasit (Trichodina, Gyrodactylus, Dactylogyrus, Chilodonella, and Argulus) dan endoparasit (Anisakis). Kata kunci: Parasit Komoditas Perikanan BKIPMHP Semarang