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Antonina Yashchenko

Bio: Antonina Yashchenko is an academic researcher from Danylo Halytsky Lviv National Medical University. The author has contributed to research in topics: Lectin & Sodium hydrosulfide. The author has an hindex of 4, co-authored 18 publications receiving 40 citations.

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Journal ArticleDOI
TL;DR: H2S donors prevent GM age-related malfunctions by enhancement of CBS, CSE, and TST expression against fructose excess injury though reduction of oxidative damage.
Abstract: Objective Excess of fructose consumption is related to life-treating conditions that affected more than a third of the global population. Therefore, to identify a newer therapeutic strategy for the impact prevention of high fructose injury in age-related malfunctions of the gastric mucosa (GM) in the animal model is important. Methods Adult and aged male rats were divided into control groups (standard diet, SD) and high fructose diet (HFD) groups; acute water immersion restraint stress (WIRS) was induced for evaluation of GM adaptive response and effects of testing the therapeutic potential of H2S-releasing compounds (H2S donors). Histological examination of gastric damage was done on hematoxylin-eosin stained slides. Cystathionine beta-synthase (CBS), Cystathionine gamma-lyase (CSE), and Thiosulfate-dithiol sulfurtransferase (TST) activities and oxidative index were assessed during exogenous administration of H2S donors: sodium hydrosulfide (NaHS) and the novel hybrid H2S-releasing aspirin (ATB-340). The results showed that HFD increased gastric damage in adult and aged rats. HFD-associated malfunction characterized by low activities of H2S key enzymes, inducing increased oxidation. Pretreatment with NaHS, ATB-340 of aged rats in the models of HFD, and WIRS attenuated gastric damage in contrast to vehicle-treated group (p < 0.05). The effect of ATB-340 was characterized by reverse oxidative index and increased CBS, CSE, and TST activities. In conclusion, H2S donors prevent GM age-related malfunctions by enhancement of CBS, CSE, and TST expression against fructose excess injury though reduction of oxidative damage.

12 citations

Journal ArticleDOI
TL;DR: NPL lectin-NaGdF4:Eu3+ conjugated NC permitted distinct identification of contours of the melanoma tissue on histological sections using red excitation at 590-610 nm and near infrared emission of 700-720 nm, of potential practical significance for development of glycans-conjugated nanoparticles to be used for in vivo visualization of melanoma tumor.
Abstract: Aim To develop specific fluorescent markers for melanoma tumor visualization, which would provide high selectivity and reversible binding pattern, by the use of carbohydrate-recognizing proteins, lectins, combined with the physical ability for imaging deep in the living tissues by utilizing red and near infrared fluorescent properties of specific rare-earth doped nanocrystals (NC).

7 citations

Journal ArticleDOI
TL;DR: It was detected that postnatal morphogenesis of rat testis is accompanied by active glycoconjugates rearrangement, and most intense lectin labeling was characteristic for fetal Leydig cells, number of which reduced significantly on the 1st postnatal day.
Abstract: A panel of 15 peroxidase labeled lectins, supplemented with haematoxylin-eosin staining and PAS-reaction, were used to study modifications of carbohydrates in rat testis during postnatal morphogenesis, including prenatal day 20th, postnatal days 1st, 20th, 40th, in comparison to the adult rat testis. Tissue samples were fixed in Bouin’s fluid and embedded in paraffin. Lectin panel included Con A, PSA, GNA, NPA, PNA, VAA, PIFA, CNFA, CCRA, SBA, HPA, MPFA, WGA, SNA and LABA. It was detected that postnatal morphogenesis of rat testis is accompanied by active glycoconjugates rearrangement. In late prenatal and early postnatal development most intense lectin labeling was characteristic for fetal Leydig cells, number of which reduced significantly on the 1st postnatal day. Highest selectivity of these cells binding was documented with PSA, GNA and NPA. On postnatal day 20th was detected strong reactivity of developing spermatocytes with PSA, CNFA, HPA and WGA, and to a lesser extent– with other used lectins. This lectin binding increased on the postnatal day 40th, covering all subsets of multilayered spermatogenic epithelium in within the seminiferous tubules. Since postnatal day 40th onwards granule- and capstage pro-acrosomes, early and late acrosomes demonstrated strong reactivity with PNA, VAA, SBA, HPA, CNFA and SNA; most selective acrosomes labeling was detected with PNA and SBA. Majority of used lectins strongly reacted with glycoconjugate deposits, located in the adlumenal compartments of seminiferous tubules. Three original lectin preparartions (LABA, MPFA, PIFA) proved to be useful tools in andrology research.

6 citations

Journal ArticleDOI
TL;DR: In this paper, the effects of exogenous NaHS (5.6 mg/kg/day for 9 days) versus vehicle on mesentery changes were investigated and it was shown that pretreatment with NaHS inhibited MA and mitochondria alterations in aged rats exposed to HFD and WIRS, lowered TBARS, and enhanced H2S enzyme activities in contrast to the vehicle-treated group.
Abstract: A high fructose diet (HFD) and advanced age are key factors for the gradual loss of physiological integrity of adipose tissue. Endogenous hydrogen sulfide (H2S) has beneficial effects on cytoprotection and redox balance. But its interactive effects on age-related damage of mesenteric vessels and connective and adipose tissues (MA) during HFD which could be the base of the development of effective physiological-based therapeutic strategy are unknown. The aim of study was to investigate age- and HFD-induced mesenteric cellular changes and activities of enzymes in H2S synthesis and to test the effects of sodium hydrosulfide (NaHS) which is considered an H2S donor on them. Adult and aged male rats on a standard diet (SD) or 4-week HFD were exposed to acute water-immersion restraint stress (WIRS) for evaluation of mesenteric subcellular and cellular adaptive responses by electron microscopy. The effects of exogenous NaHS (5.6 mg/kg/day for 9 days) versus vehicle on mesentery changes were investigated. Serum glucose level, thiobarbituric acid reactive substances (TBARS), and activities of cystathionine γ-lyase (CSE) and cystathionine β-synthase (CBS), thiosulfate-dithiol sulfurtransferase (TST), and sulfite oxidase (SO) were examined by spectrophotometry. In both adult and aged SD groups, treatment with NaHS protected mesenteric cells after WIRS. In both groups, the treatment with NaHS also protected MA mitochondria, microvascular endothelial and sub-endothelial structures, and fibroblasts versus the vehicle-treated group that had signs of damage. HFD increased MA injury and mitochondrial changes in both aged and adult rats. HFD-associated malfunction is characterized by low activities of CSE, CBS, TST, SO, and increased TBARS. Finally, we demonstrated that pretreatment with NaHS inhibited MA and mitochondria alterations in aged rats exposed to HFD and WIRS, lowered TBARS, and enhanced H2S enzyme activities in contrast to the vehicle-treated group. Mitochondrial integrity alterations, endothelial damage, and redox imbalance are key factors for rat mesenteric adipose tissue damage during advanced age. These alterations and MA hypertrophic changes retain the central for HFD-induced damage. Moreover, H2S signaling contributes to MA and mitochondria redox balance that is crucial for advanced age and HFD injury. The future study of H2S donors' effects on mesenteric cells is fundamental to define novel therapeutic strategies against metabolic changes.

5 citations

Journal Article
TL;DR: A set of lectins with different carbohydrate affinities is used to investigate impairment in rat liver glycoconjugates influenced by streptozotocin-induced diabetes mellitus and demonstrate the applicability of the original fucose-specific lectin preparation to experimental histopathology.
Abstract: Diabetes mellitus (DM) currently belongs to the most widespread human pathologies, affecting about 4% of the world adult population. Despite the pivotal role of the liver in the development of metabolic disorders, the influence of DM on hepatic glycoconjugates remains obscure. The aim of the present investigation was to use a set of lectins with different carbohydrate affinities to investigate impairment in rat liver glycoconjugates influenced by streptozotocin-induced diabetes mellitus. The lectin panel included 7 conventional lectins – Con A, SNA, RCA, WGA, PNA, SBA, and HPA, supplemented with the original fucose-specific lectin preparation from Laburnum anagyroides bark (LABA). Tissue samples were fixed in 4% neutral formalin, embedded in paraffin, and subjected to lectin-peroxidase-diaminobenzidine staining. In control rats a strong reactivity against Con A, LABA, SBA and SNA with cytoplasmic granularities of hepatocytes was detected, while RCA, WGA and HPA showed a strong reactivity with vascular endothelium, and WGA and HPA with bile capillaries. Experimental diabetes was associated with a redistribution of Con A and LABA receptor sites from centrolobular hepatocytes to hepatocytes with peripheral localization. Among the most remarkable observations was DMinduced exposure of lectin reactivity with hepatocyte and endothelial cell nuclei. The endothelial lining of sinusoidal hemocapillaries, of central veins, and portal tract vessels also displayed a significant and differential rearrangement of carbohydrate determinants when influenced by DM. Diabetes-induced activation of Kupffer cells was accompanied by the expression of SNA, PNA and SBA receptor sites within the cytoplasm of these cells, which was lectin-negative in control specimens. The results reported provide a new insight into the pathogenesis of DM-induced impairment of hepatic carbohydrates, and demonstrate the applicability of the original fucose-specific lectin preparation to experimental histopathology.

4 citations


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TL;DR: The vascular system involves the heart and blood vessels, which include arteries, which are tubes that transport blood from your heart to the rest of your body, and veins, which return the blood to your heart.
Abstract: BY DR. PAUL DIMUZIO The vascular system involves the heart and blood vessels. The blood vessels include arteries, which are tubes that transport blood from your heart to the rest of your body, and veins, which return the blood to your heart. This complex system is involved in many diverse functions, but the most important is transporting vital oxygen and nutrients to your tissues and organs. ARTERIES are thicker and are prone to developing blockages or dilations. VEINS are thinner and have valves that keep the blood moving back toward the heart. The larger deep veins of the legs can develop clots. Blood vessels are composed of three layers.

37 citations

Journal ArticleDOI
TL;DR: It is shown here that SIRT1 in endothelial cells is a key mediator of pro-angiogenic signals secreted from myocytes that have implications for improving blood flow to organs and tissues, increasing performance, and reestablishing a virtuous cycle of mobility in aged individuals.
Abstract: With aging there is a notable decline in capillary density and blood flow contributing to mortality and morbidity. The use of NAD+ boosters to reverse aspects of aging, is in part, through the mechanism of activating sirtuin deacylases (SIRT1–SIRT7) that mediate the benefits of exercise and calorie restriction (CR). We show here that SIRT1 in endothelial cells is a key mediator of pro-angiogenic signals secreted from myocytes. Treatment of mice with the NAD+ precursor nicotinamide mononucleotide (NMN) improves blood flow and increases endurance in advanced aged mice by promoting SIRT1-dependent increases in capillary density, an effect augmented by exercise or increasing the levels of hydrogen sulfide (H2S), a CR mimetic and regulator of endothelial NAD+ levels. These findings have implications for improving blood flow to organs and tissues, increasing performance, and reestablishing a virtuous cycle of mobility in aged individuals.

34 citations

Journal ArticleDOI
Brian Godman1, Brian Godman2, Brian Godman3, Mainul Haque4, Trudy Leong, Eleonora Allocati5, Santosh Kumar, Salequl Islam6, Jaykaran Charan7, Farhana Akter8, Amanj Kurdi1, Amanj Kurdi9, Amanj Kurdi10, Carlos Vassalo11, Muhammed Abu Bakar, Sagir Abdur Rahim, Nusrat Sultana, Farzana Deeba, M. A. Halim Khan12, A. B.M.Muksudul Alam12, Iffat Jahan13, Zubair Mahmood Kamal14, Humaira Hasin15, Munzur-E-Murshid16, Shamsun Nahar6, M. E. Haque, Siddhartha Dutta7, Jha Pallavi Abhayanand7, Rimple Jeet Kaur7, Godfrey Mutashambara Rwegerera17, Renata Cristina Rezende Macedo do Nascimento18, Isabella Piassi Godói19, Mohammed Irfan20, Adefolarin A. Amu, Patrick Matowa, Joseph Acolatse, Robert Incoom, Israel Abebrese Sefah21, Jitendra Acharya22, Sylvia Opanga23, Lisper Wangeci Njeri24, David Kimonge23, Hye Young Kwon25, Seung Jin Bae26, Karen Koh Pek Khuan, Abdullahi Rabiu Abubakar27, Ibrahim Haruna Sani28, Tanveer Ahmed Khan, Shahzad Hussain, Zikria Saleem29, Oliver Ombeva Malande30, Thereza Piloya-Were28, Rosana Gambogi, Carla Hernandez Ortiz, Luke Alutuli, Aubrey Chichonyi Kalungia31, Iris Hoxha32, Vanda Marković-Peković33, Biljana Tubić33, Guenka Petrova34, Konstantin Tachkov34, Ott Laius, András Harsányi35, András Inotai36, Arianit Jakupi, Svens Henkuzens, Kristina Garuoliene37, Jolanta Gulbinovič37, Magdalene Wladysiuk38, Jakub Rutkowski, Ileana Mardare39, Jurij Fürst, Stuart McTaggart, Sean MacBride-Stewart, Caridad Pontes40, Caridad Pontes41, Corinne Zara, Eunice Twumwaa Tagoe10, Rita Banzi5, Janney Wale, Mihajlo Jakovljevic42, Mihajlo Jakovljevic43 
TL;DR: In this paper, the authors assess current utilisation rates for insulins, especially long-acting insulin analogues, and the rationale for patterns seen, across multiple countries to inform strategies to enhance future utilisation of insulin analogue biosimilars to benefit all key stakeholders.
Abstract: Background: Diabetes mellitus rates continue to rise, which coupled with increasing costs of associated complications has appreciably increased global expenditure in recent years. The risk of complications are enhanced by poor glycaemic control including hypoglycaemia. Long-acting insulin analogues were developed to reduce hypoglycaemia and improve adherence. Their considerably higher costs though have impacted their funding and use. Biosimilars can help reduce medicine costs. However, their introduction has been affected by a number of factors. These include the originator company dropping its price as well as promoting patented higher strength 300 IU/ml insulin glargine. There can also be concerns with different devices between the manufacturers. Objective: To assess current utilisation rates for insulins, especially long-acting insulin analogues, and the rationale for patterns seen, across multiple countries to inform strategies to enhance future utilisation of long-acting insulin analogue biosimilars to benefit all key stakeholders. Our approach: Multiple approaches including assessing the utilisation, expenditure and prices of insulins, including biosimilar insulin glargine, across multiple continents and countries. Results: There was considerable variation in the use of long-acting insulin analogues as a percentage of all insulins prescribed and dispensed across countries and continents. This ranged from limited use of long-acting insulin analogues among African countries compared to routine funding and use across Europe in view of their perceived benefits. Increasing use was also seen among Asian countries including Bangladesh and India for similar reasons. However, concerns with costs and value limited their use across Africa, Brazil and Pakistan. There was though limited use of biosimilar insulin glargine 100 IU/ml compared with other recent biosimilars especially among European countries and Korea. This was principally driven by small price differences in reality between the originator and biosimilars coupled with increasing use of the patented 300 IU/ml formulation. A number of activities were identified to enhance future biosimilar use. These included only reimbursing biosimilar long-acting insulin analogues, introducing prescribing targets and increasing competition among manufacturers including stimulating local production. Conclusions: There are concerns with the availability and use of insulin glargine biosimilars despite lower costs. This can be addressed by multiple activities.

20 citations

Journal ArticleDOI
TL;DR: H2S donors prevent GM age-related malfunctions by enhancement of CBS, CSE, and TST expression against fructose excess injury though reduction of oxidative damage.
Abstract: Objective Excess of fructose consumption is related to life-treating conditions that affected more than a third of the global population. Therefore, to identify a newer therapeutic strategy for the impact prevention of high fructose injury in age-related malfunctions of the gastric mucosa (GM) in the animal model is important. Methods Adult and aged male rats were divided into control groups (standard diet, SD) and high fructose diet (HFD) groups; acute water immersion restraint stress (WIRS) was induced for evaluation of GM adaptive response and effects of testing the therapeutic potential of H2S-releasing compounds (H2S donors). Histological examination of gastric damage was done on hematoxylin-eosin stained slides. Cystathionine beta-synthase (CBS), Cystathionine gamma-lyase (CSE), and Thiosulfate-dithiol sulfurtransferase (TST) activities and oxidative index were assessed during exogenous administration of H2S donors: sodium hydrosulfide (NaHS) and the novel hybrid H2S-releasing aspirin (ATB-340). The results showed that HFD increased gastric damage in adult and aged rats. HFD-associated malfunction characterized by low activities of H2S key enzymes, inducing increased oxidation. Pretreatment with NaHS, ATB-340 of aged rats in the models of HFD, and WIRS attenuated gastric damage in contrast to vehicle-treated group (p < 0.05). The effect of ATB-340 was characterized by reverse oxidative index and increased CBS, CSE, and TST activities. In conclusion, H2S donors prevent GM age-related malfunctions by enhancement of CBS, CSE, and TST expression against fructose excess injury though reduction of oxidative damage.

12 citations