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Anuradha Biswas

Bio: Anuradha Biswas is an academic researcher from University of Calcutta. The author has contributed to research in topics: Phosphatidylcholine. The author has an hindex of 1, co-authored 1 publications receiving 9 citations.

Papers
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Journal ArticleDOI
TL;DR: The acyl group distributions in the 1- and 2-positions of hen egg phosphatidylcholine obtained from the gas-liquid chromatographic analysis of the methyl ester corresponding to the lyso and free fatty acid band agreed with those obtained by the method of Wells and Hanahan.

9 citations


Cited by
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Journal ArticleDOI
TL;DR: The results indicate that sPLA(2)-III can create white matter pathologies that are remyelinated by Schwann cells 2 to 3 weeks after injury, and is the first report of a reaching task being able to discriminate between various grades of cervical white matter damage and varying extents of recovery.

38 citations

Journal ArticleDOI
TL;DR: In this paper, a method for the lipase deacylation of triglycerides is described in which 0.1 − 0.2 ml of the enzyme solution in 1 M tris buffer (pH 8.2) containing 2 − 4 mg of protein is applied as a band on a 0.5mm thick silica gel thin-layer chromatographic plate.

23 citations

Journal ArticleDOI
01 Dec 1983-Lipids
TL;DR: A rapid method for the stereospecific analysis of triglycerides based on enzymatic hydrolysis on thin layer plates was applied to a number of GlyCine max, Glycine soya, Avena sativa and Avena sterilis strains and large deviations from the common triglyceride pattern were not found.
Abstract: A rapid method for the stereospecific analysis of triglycerides based on enzymatic hydrolysis on thin layer plates was applied to a number ofGlycine max, Glycine soya, Avena sativa andAvena sterilis strains. The percentage of each fatty acid on thesn-1,sn-2- andsn-3-positions was linearly related to the total percentage of the fatty acid in the triglyceride. Large deviations from the common triglyceride pattern were not found.

20 citations

Journal ArticleDOI
01 Dec 1985-Lipids
TL;DR: The PE component of rabbit sperm phospholipids appears to differ from that of the other cells in having the previously unreported diplasmalogen as its major constituent.
Abstract: The question of whether diplasmalogens [1,2-di(O-1′-alkenyl) phosphatidyl derivatives] make up part of the plasmalogen component of cell phospholipids was examined using rabbit epididymal spermatozoa. These cells are readily obtained as a highly homogeneous suspension and long have been known to have high plasmalogen content. Phospholipids were determined by thin layer chromatography (TLC) with CuSO4 staining. Plasmalogens were determined by hydrolysis of the phospholipids with TCA/HCl, followed by TLC and CuSO4 staining. Ethanolamine derivatives were determined by ninhydrin. The phosphatidylethanolamine (PE) content of these cells was 29±2 μg/108 cells, 90% of which was assayed as diplasmalogen and 10% as diacyl PE. No monoplasmalogen could be detected. The presence of diplasmalogen as the major component of PE was given further support from infrared and proton nuclear magnetic resonance (1H-NMR) spectroscopy, which showed the presence of O-1′-alkenyl substituents but near absence of O-acyl substituents. The phosphatidylcholine (PC) content of the cells was 104±5 μ/108 cells, of which 50% was monoplasmalogen with the 1′-alkenyl group on the 2 position of the glycerol moiety. No diplasmalogen was found in PC. The other phospholipids in rabbit sperm were phosphatidylglycerol (PG), cardiolipin (CL), sphingomyelin (SP) and lysophosphatidylcholine (LPC). Phosphatidylserine (PS) and phosphatidylinositol (PI) were present at the limits of detectability of the TLC method. None of these phospholipids contained plasmalogen. The PE component of rabbit sperm phospholipids appears to differ from that of the other cells in having the previously unreported diplasmalogen as its major constituent.

19 citations

Journal ArticleDOI
TL;DR: In this paper, the effects of microwave roasting on the composition and positional distribution of the fatty acids were studied not only for phospholipid composition but also for positional distribution.
Abstract: Whole soybeans were exposed to microwave roasting for 6, 12, and 20 min at a frequency of 2,450 MHz and were studied not only for phospholipid composition but also for positional distribution of the fatty acids. During microwave roasting, the greatest rate of phospholipid losses (P<0.05) was observed in phosphatidylethanolamine (PE), followed by phosphatidylcholine (PC) and phosphatidylinositol (PI), respectively. Therefore, the effects of microwave roasting on the composition and positional distribution of the fatty acids are likely clearer in PE than in PC or PI. However, the principal characteristics for the positional distribution of fatty acids are still retained during microwave roasting: unsaturated fatty acids, especially linoleic, are predominantly concentrated in the 2-position, and saturated fatty acids, especially palmitic, primarily occupy the 1-position after 12 or 20 min of roasting. The results suggest that unsaturated fatty acids located in the 2-position are significantly protected from microwave roasting.

15 citations