Archita Gupta

Bio: Archita Gupta is an academic researcher from Birla Institute of Technology, Mesra. The author has contributed to research in topics: Medicine & Nanocrystalline material. The author has an hindex of 4, co-authored 10 publications receiving 29 citations. Previous affiliations of Archita Gupta include Birla Institute of Technology and Science.

Nitrate Reductase mediated synthesis of surface passivated nanogold as broad-spectrum antibacterial agent

Abstract: The green synthesis of gold nanoparticles has attracted tremendous interest owing to their unique physicochemical properties and widespread applications which are primarily size-dependent. The stability, less reaction time, and use of biological resources as novel nanofactories as an alternative to conventional synthesis strategies are the main objectives of green synthesis approaches. However, to attain size-controlled synthesis from the biogenic route is still a challenge. Hence, use of nontoxic stabilizers becomes increasingly essential. Herein, we describe an emerging, simple, nonconventional approach to synthesize stable and size-controlled biogenic nanogold using cell lysate supernatant containing nitrate reductase of Bacillus licheniformis in the presence of Tween 20 and dodecanethiol respectively. The face-centered central composite design used for the optimization of gold nanoparticles (AuNPs) biosynthesis. The maximum AuNPs biosynthesis obtained using the optimized media variables, glucose (2.1 g/L), peptone (14.05 g/L), yeast extract (4.14 g/L), and potassium nitrate (3.91 g/L) was 0.769 a.u. Highly stable monodispersed nanogold of 10.4 ± 0.6 nm and 12.5 ± 0.9 nm sizes arranged in ordered self-assembly was obtained. The stability profile and kinetics of bioreduction was evaluated with respect to time, and the involvement of the nitrate reductase enzyme in bioreduction was validated by inhibitor study. The physicochemical properties of biogenic nanoparticles were characterized using multiple spectroscopy and microscopy techniques. The obtained nanogold demonstrated excellent bactericidal property against both gram-negative and gram-positive bacteria in size-dependent manner and thus could find tremendous utility in clinical, biological, and environmental applications as a broad-spectrum antibacterial agent.

Biofabrication of gold nanoparticles with bone remodeling potential: an in vitro and in vivo assessment

Abstract: Recent thrust in the biomaterials research has shifted towards developing bioactive and resorbable scaffolds with nanotechnological interventions. Next-generation bone scaffolds need to be multifunctional with desired osteo-integration ability, conformed resorption rate and in vivo imaging potential. In this study, we synthesized biogenic multifunctional gold nanoparticles (AuNPs) using immobilized crude enzyme (ImCE) of Bacillus licheniformis. The multifunctional characteristics of AuNPs and reusability of ImCE for AuNP synthesis can make the process and application cost-effective. ImCE catalyzed the bioreduction of gold ions to AuNPs of 18.4 ± 3.3 nm. AuNPs showed good hemocompatibility and antioxidant property without any toxic effects. Further, AuNPs demonstrated the increase in alkaline phosphatase activity and calcium deposition through MC3T3-E1 murine preosteoblast cell line and rat bone marrow stromal cells (BMSCs). The osteoinductive effect of AuNPs was also investigated in vivo wherein the composite cryogel scaffold functionalized with bone morphogenic protein-2 (rhBMP-2), zoledronic acid (ZA), and AuNPs was implanted in a muscle pouch of Wistar rats. AuNPs had synergistic effect on bone formation along with rhBMP-2 and ZA as seen from micro-computed tomography and histological analysis. The AuNPs were found to exhibit autofluorescence property and enhanced the degradation of scaffold at the implanted site in vivo. The developed process of using ImCE presents a novel approach for biogenic fabrication of AuNPs with the potential orthopedic application.

The Review of Natural Products

Abstract: The Review of Natural Products is the foremost source of current natural product information for health care professionals. More than 350 in-depth monographs are included, based on scientific research, not just anecdotal information. The Review of Natural Products provides detailed information about natural products, including their botany, history, chemistry, pharmacology, medicinal uses, toxicology, and patient information. It also includes significantly documented drug interactions.Provides up-to-date, objective assessment of the latest medical and scientific studies on natural products, including medically active foods (nutraceuticals).

Fish-scale bio-inspired multifunctional ZnO nanostructures

Abstract: Scales provide optical disguise, low water drag and mechanical protection to fish, enabling them to survive catastrophic environmental disasters, predators and microorganisms. The unique structures and stacking sequences of fish scales inspired the fabrication of artificial nanostructures with salient optical, interfacial and mechanical properties. Herein, we describe fish-scale bio-inspired multifunctional ZnO nanostructures that have similar morphology and structure to the cycloid scales of the Asian Arowana. These nanostructured coatings feature tunable light refraction and reflection, modulated surface wettability and damage-tolerant mechanical properties. The salient properties of these multifunctional nanostructures are promising for applications in: - (i) optical coatings, sensing or lens arrays for use in reflective displays, packing, advertising and solar energy harvesting; - (ii) self-cleaning surfaces, including anti-smudge, anti-fouling and anti-fogging, and self-sterilizing surfaces, and; - (iii) mechanical/chemical barrier coatings. This study provides a low-cost and large-scale production method for the facile fabrication of these bio-inspired nanostructures and provides new insights for the development of novel functional materials for use in 'smart' structures and applications.

Potential application of gold nanoparticles in food packaging: a mini review

Abstract: In the past few decades, there have been remarkable advances in our knowledge of gold nanoparticles (AuNPs) and synthesizing methods. AuNPs have become increasingly important in biomedical and industrial applications. As a newly implemented method, AuNPs are being used in nanopackaging industries for their therapeutic and antibacterial characteristics as well as their inert and nontoxic nature. As with other NPs, AuNPs have privileges and disadvantages when utilized in the food sector, yet a significant body of research has shown that, due to the specific nontoxic characteristics, AuNPs could be used to address other NP flaws. In this mini review, we present synthesizing methods, food industry applications, and mechanisms of action of gold nanoparticles. Regarding the investigations, gold nanoparticles can play a major role to reduce microbial load in foodstuff and therefore can be implemented in food packaging as an effective approach.