A
Ario de Marco
Researcher at European Bioinformatics Institute
Publications - 46
Citations - 3069
Ario de Marco is an academic researcher from European Bioinformatics Institute. The author has contributed to research in topics: Medicine & Biology. The author has an hindex of 24, co-authored 34 publications receiving 2691 citations. Previous affiliations of Ario de Marco include University of Nova Gorica & European Institute of Oncology.
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Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli.
TL;DR: This article reviews the available strategies for exploiting the physiological mechanisms of bactera to produce properly folded disulfide-bonded proteins and recommends several strategies aimed at the formation and at the quality control of disulfides in the oxidizing periplasm.
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Symmetric dimethylation of H3R2 is a newly identified histone mark that supports euchromatin maintenance
Valentina Migliori,Julius Muller,Sameer Phalke,Diana Low,Marco Bezzi,Marco Bezzi,Wei Chuen Mok,Sanjeeb Kumar Sahu,Jayantha Gunaratne,Paola Capasso,Christian Bassi,Christian Bassi,Valentina Cecatiello,Ario de Marco,Walter Blackstock,Vladimir A. Kuznetsov,Bruno Amati,Bruno Amati,Marina Mapelli,Ernesto Guccione,Ernesto Guccione +20 more
TL;DR: H3R2me2s is identified as a previously unknown mark that keeps genes poised in euchromatin for transcriptional activation upon cell-cycle withdrawal and differentiation in human cells.
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Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli.
TL;DR: The engineered E. coli strains and the two-step procedure presented here led to a remarkable increase in the solubility of a various recombinant proteins and should be applicable to a wide range of target proteins produced in biotechnology.
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NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies
Sandrine Moutel,Sandrine Moutel,Sandrine Moutel,Nicolas Bery,Nicolas Bery,Virginie Bernard,Laura Keller,Laura Keller,Emilie Lemesre,Emilie Lemesre,Ario de Marco,Laetitia Ligat,Jean-Christophe Rain,Gilles Favre,Gilles Favre,Aurélien Olichon,Aurélien Olichon,Franck Perez,Franck Perez +18 more
TL;DR: This work describes the first fully synthetic phage display library of humanized llama single domain antibody (NaLi-H1: Nanobody Library Humanized 1), based on a humanized synthetic singledomain antibody (hs2dAb) scaffold optimized for intracellular stability, which provides high affinity binders without animal immunization.
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Native folding of aggregation-prone recombinant proteins in Escherichia coli by osmolytes, plasmid- or benzyl alcohol–overexpressed molecular chaperones
TL;DR: Combined strategies of osmolyte-induced native folding with heat-, BA-, or plasmid-induced chaperone coexpression can be thought to optimize yields of natively folded recombinant proteins in bacteria, for research and biotechnological purposes.