Author
Arnold J. Berk
Bio: Arnold J. Berk is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: Gene & Endonuclease. The author has an hindex of 4, co-authored 4 publications receiving 3597 citations.
Topics: Gene, Endonuclease, Adenovirus early region 1A, DNA, RNA
Papers
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TL;DR: A simple and sensitive method for detecting, sizing and mapping RNA transcripts from viral or cloned DNAs has been developed and used to examine the cytoplasmic transcripts produced during the early phase of adenovirus 2 (Ad2) infection of HeLa cells.
2,367 citations
TL;DR: It is demonstrated that the expression of many early adenovirus mRNAs is dependent upon the activity of a pre-early viral product, which is defective in adanovirus 5 host range (Ad hr) group I mutants.
642 citations
TL;DR: The structure of adenovirus 2 (Ad2) cytoplasmic RNAs produced during the early phase of infection is defined and it is suggested that, like the SV40 tumor antigens, the polypeptides encoded by these Ad2 mRNAs have an identical amino acid sequence at their N terminal ends, but have different C terminal sequences.
512 citations
TL;DR: The concentration of early mRNAs observed in cells mixedly infected with unirradiated and irradiated virions suggests that initiation of transcription is the rate-limiting step in the production of the early m RNAs, and that this rate is independent of the number of viral templates within the nucleus.
88 citations
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TL;DR: In this paper, a simple and efficient method for synthesizing pure single stranded RNAs of virtually any structure is described, based on the unusually specific RNA synthesis by bacteriophage SP6 RNA polymerase which initiates transcription exclusively at an SP6 promoter.
Abstract: A simple and efficient method for synthesizing pure single stranded RNAs of virtually any structure is described. This in vitro transcription system is based on the unusually specific RNA synthesis by bacteriophage SP6 RNA polymerase which initiates transcription exclusively at an SP6 promoter. We have constructed convenient cloning vectors that contain an SP6 promoter immediately upstream from a polylinker sequence. Using these SP6 vectors, optimal conditions have been established for in vitro RNA synthesis. The advantages and uses of SP6 derived RNAs as probes for nucleic acid blot and solution hybridizations are demonstrated. We show that single stranded RNA probes of a high specific activity are easy to prepare and can significantly increase the sensitivity of nucleic acid hybridization methods. Furthermore, the SP6 transcription system can be used to prepare RNA substrates for studies on RNA processing (1,5,9) and translation (see accompanying paper).
5,732 citations
TL;DR: It is proposed that the AU sequences are the recognition signal for an mRNA processing pathway which specifically degrades the mRNAs for certain lymphokines, cytokines, and proto-oncogenes.
3,981 citations
TL;DR: A simple and sensitive method for detecting, sizing and mapping RNA transcripts from viral or cloned DNAs has been developed and used to examine the cytoplasmic transcripts produced during the early phase of adenovirus 2 (Ad2) infection of HeLa cells.
2,367 citations
TL;DR: It is proposed that the IVS portion of the RNA has several enzyme-like properties that enable it to break and reform phosphodiester bonds and that enzymes, small nuclear RNAs and folding of the pre-rRNA into an RNP are unnecessary for these reactions.
2,077 citations
TL;DR: A regulatory linkage between the function of two oncogenes--c-myc and c-sis--the latter being the putative structural gene for PDGF is suggested, consistent with a model that a labile protein may regulate c- myc levels in these cells.
2,073 citations