Arthur Poester Cordeiro

Bio: Arthur Poester Cordeiro is an academic researcher from Universidade Federal de Santa Catarina. The author has contributed to research in topics: Cytotoxicity & Liposome. The author has an hindex of 4, co-authored 9 publications receiving 39 citations.

Diethyldithiocarbamate encapsulation reduces toxicity and promotes leishmanicidal effect through apoptosis-like mechanism in promastigote and ROS production by macrophage.

Abstract: The use of compounds from natural or synthetic sources and nanotechnology may represent an alternative to develop new drugs for the leishmaniasis treatment. DETC is an inhibitor of the SOD1 enzyme, which leads to increased ROS production, important for the elimination of Leishmania. Thus, our objective was to assess the leishmanicidal in vitro effect of free Diethydithiocarbamate (DETC) and DETC loaded in beeswax-copaiba oil nanoparticles (DETC-Beeswax-CO Nps) on L. amazonensis forms and elucidate the possible mechanisms involved in the parasite death. DETC-Beeswax-CO Nps presented size below 200 nm, spherical morphology, negative zeta potential, and high encapsulation efficiency. Free DETC reduced the viability of promastigotes and increase ROS production, lower the mitochondrial membrane potential, cause phosphatidylserine exposure, and enhance plasma membrane permeability, in addition to promoting morphological changes in the parasite. Free DETC proved toxic in the assessment of toxicity to murine macrophages, however, the encapsulation of this compound was able to reduce these toxic effects on macrophages. DETC-Beeswax-CO Nps exerted anti-amastigote effect by enhancing the production of ROS, superoxide anion, TNF-α, IL-6, and reduced IL-10 in macrophages. Therefore, free DETC induces antipromastigote effect by apoptosis-like; and DETC-Beeswax-CO Nps exerted anti-leishmanial effect due to pro-oxidant and pro-inflammatory response.

Preparation of Solid Lipid Nanoparticles and Nanostructured Lipid Carriers for Drug Delivery and the Effects of Preparation Parameters of Solvent Injection Method

Abstract: Solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) have emerged as potential drug delivery systems for various applications that are produced from physiological, biodegradable, and biocompatible lipids. The methods used to produce SLNs and NLCs have been well investigated and reviewed, but solvent injection method provides an alternative means of preparing these drug carriers. The advantages of solvent injection method include a fast production process, easiness of handling, and applicability in many laboratories without requirement of complicated instruments. The effects of formulations and process parameters of this method on the characteristics of the produced SLNs and NLCs have been investigated in several studies. This review describes the methods currently used to prepare SLNs and NLCs with focus on solvent injection method. We summarize recent development in SLNs and NLCs production using this technique. In addition, the effects of solvent injection process parameters on SLNs and NLCs characteristics are discussed.

Magnetic Nanoparticles: From Synthesis to Theranostic Applications

Abstract: Over the past few years, engineered inorganic nanoparticles have been studied, researched, and applied extensively in the biomedical field because resultant platforms increase the usage of nanopart...

Recent Advances in Nanotechnology for the Treatment of Melanoma.

Abstract: Melanoma is one of the most aggressive forms of skin cancer, with few possibilities for therapeutic approaches, due to its multi-drug resistance and, consequently, low survival rate for patients. Conventional therapies for treatment melanoma include radiotherapy, chemotherapy, targeted therapy, and immunotherapy, which have various side effects. For this reason, in recent years, pharmaceutical and biomedical research has focused on new sito-specific alternative therapeutic strategies. In this regard, nanotechnology offers numerous benefits which could improve the life expectancy of melanoma patients with very low adverse effects. This review aims to examine the latest advances in nanotechnology as an innovative strategy for treating melanoma. In particular, the use of different types of nanoparticles, such as vesicles, polymers, metal-based, carbon nanotubes, dendrimers, solid lipid, microneedles, and their combination with immunotherapies and vaccines will be discussed.

Protein corona-induced aggregation of differently sized nanoplastics: impacts of protein type and concentration

Abstract: Nanoplastic contamination is one of the pressing environmental concerns globally. Among many environmental factors in aquatic systems, ubiquitous proteins are expected to affect the physicochemical properties of nanoplastics and subsequently influence their fate, transport, and toxicity. Here, time-resolved dynamic light scattering was used to investigate the comparative effects of negatively charged bovine serum albumin (BSA) and positively charged bovine trypsin (TRY) on polystyrene nanoplastic (PSNP) aggregation. The critical coagulation concentrations (NaCl) of 20 and 100 nm PSNPs decreased from 311 and 361 mM to 10 and 43 mM after interacting with TRY, respectively, mainly due to the additional electrostatic attractive force and intramolecular bridging. The attachment efficiencies of BSA–PSNP conjugates decreased from 1 to 0 with increasing electrolyte concentration, suggesting that the patch-charge attractive force may be screened by steric repulsion. At a relatively high level of protein (>10 mg L−1), PSNPs remained stable in BSA solution, but aggregated quickly in TRY solution. Results clearly showed that the aggregation of nanoplastics was highly related to the electrical charge of the proteins. Compared with 100 nm PSNPs, BSA stabilized 20 nm PSNPs more effectively, whereas TRY destabilized 20 nm PSNPs more effectively, indicating that the smaller PSNPs (20 nm) were more susceptible to the co-occurrence of proteins. This work highlighted the necessity of accounting for the protein type and particle size when evaluating the aggregation state and potential risk of emerging nanoplastics in aquatic systems.