scispace - formally typeset
Search or ask a question
Author

Atsuko Shinohara

Bio: Atsuko Shinohara is an academic researcher from Juntendo University. The author has contributed to research in topics: Pregnancy & Sperm. The author has an hindex of 19, co-authored 60 publications receiving 1339 citations.
Topics: Pregnancy, Sperm, Selenium, Gadolinium, Excretion


Papers
More filters
Journal ArticleDOI
TL;DR: The results suggest that L-OHP encapsulated within TF-PEG-liposomes has potential for cancer therapy.

232 citations

Journal ArticleDOI
TL;DR: High retention of 10B in tumor tissue indicates that binding and concomitant cellular uptake of the extravasated TF-PEG liposomes occurs by TF receptor and receptor-mediated endocytosis, respectively, causing tumor growth suppression in vivo upon thermal neutron irradiation.

187 citations

Journal ArticleDOI
TL;DR: Seventy male factory workers were studied, and it was observed that the Se concentration was higher in RBC, and that there was no remarkable change in the plasma.
Abstract: Seventy male factory workers were studied. The lead concentrations in their blood (Pb-B) were 16.55±11.53 μg/100 ml (range 1.5 to 50.2 μg/100 ml). The subjects were divided into three groups according to Pb-B (in μg/100 ml): group A, Pb-B ≤10 (n=22); group B, 10 20 (n= 18). The mean±S.D. in each group was 5.57±2.53, 15.02±2.75, and 32.52±9.49 μg/100 ml, respectively. Pb in plasma was 0.011±0.010, 0.017±0.033, and 0.021±0.021 μg/liter, and Pb in the RBC was 0.281±0.246, 0.701±0.325, and 1.626±0.861 μg/g Hb, respectively. In addition to Pb concentration, the concentrations of 34 elements in the plasma or in the RBC were determined. Se concentrations in RBC in each group were 0.618±0.139, 0.670±0.207, and 0.728±0.200 μg/g Hb, and the mean values were significantly different between groups A and C (p<0.05). For Se concentrations in plasma, the mean±S.D. in each group was 0.132±0.035, 0.130±0.031, and 0.126±0.021 μ g/ml, respectively, and there was no significant difference between groups. On the other hand, when the activities of total SOD, Mn-SOD, Cu, Zn-SOD, and catalase in the plasma and the activities of GSR-Px both in the plasma and in the RBC were assayed, some differences were found. The activities in GSH-Px in RBC were 17.19±5.03, 17.59±3.95, and 15.25±3.18 μmol/g Hb/min, and those in plasma were 0.069±0.032, 0.081±0.023, and 0.080±0.028 μmol/ml/min. In group C, GSR-Px activity was lower in the RBC and higher in the plasma than those in group A, and it was observed that the Se concentration was higher in RBC, and that there was no remarkable change in the plasma. Catalase activity in group C was 3.58±0.81 mgH2O2/ml/30 min, which was significantly higher than that in group A (2.81±0.90 mg H2O2/ml/30 min). Further investigation is necessary in order to explain the above results. The regular indices used for evaluating lead exposure, showed significant correlations with Pb-B: r=−0.786 vs δ-Aminolevulinic acid (ALA) dehydratase activity in blood, r=0.927 vs. inhibition rate, and r=0.339 vs. ALA in urine.

103 citations

Journal ArticleDOI
TL;DR: Oral long-term lithium treatment could attenuate p-tau-induced motor disturbance not only by inhibiting GSK-3 but also by enhancing autophagy in tauopathy model mice.

73 citations

Journal ArticleDOI
TL;DR: Logistic regression analysis demonstrated that a 1 unit increase in blood lead levels led to an increased risk of preterm birth and adverse pregnancy outcomes may occur at blood lead concentrations below the current acceptable level.
Abstract: Objectives Although occupational and environmental exposures to lead have been dramatically reduced in recent decades, adverse pregnancy outcomes have been observed at ‘acceptable’ levels of blood lead concentrations (≤10 μg/dl). Methodology Blood samples were collected from 348 singleton pregnant women, aged 16–35 years, during the first trimester of pregnancy (8–12 weeks) for lead measurement by inductively coupled plasma–mass spectrometry. Subjects were followed up and divided into two groups (preterm and full-term deliveries) according to duration of gestation. Results The average (range) and geometric means of blood lead levels were 3.8 (1.0–20.5) and 3.5 μg/dl, respectively. Blood lead level was significantly (p Conclusion Adverse pregnancy outcomes may occur at blood lead concentrations below the current acceptable level.

70 citations


Cited by
More filters
01 Jan 1980
TL;DR: In this article, the influence of diet on the distribution of nitrogen isotopes in animals was investigated by analyzing animals grown in the laboratory on diets of constant nitrogen isotopic composition and found that the variability of the relationship between the δ^(15)N values of animals and their diets is greater for different individuals raised on the same diet than for the same species raised on different diets.
Abstract: The influence of diet on the distribution of nitrogen isotopes in animals was investigated by analyzing animals grown in the laboratory on diets of constant nitrogen isotopic composition. The isotopic composition of the nitrogen in an animal reflects the nitrogen isotopic composition of its diet. The δ^(15)N values of the whole bodies of animals are usually more positive than those of their diets. Different individuals of a species raised on the same diet can have significantly different δ^(15)N values. The variability of the relationship between the δ^(15)N values of animals and their diets is greater for different species raised on the same diet than for the same species raised on different diets. Different tissues of mice are also enriched in ^(15)N relative to the diet, with the difference between the δ^(15)N values of a tissue and the diet depending on both the kind of tissue and the diet involved. The δ^(15)N values of collagen and chitin, biochemical components that are often preserved in fossil animal remains, are also related to the δ^(15)N value of the diet. The dependence of the δ^(15)N values of whole animals and their tissues and biochemical components on the δ^(15)N value of diet indicates that the isotopic composition of animal nitrogen can be used to obtain information about an animal's diet if its potential food sources had different δ^(15)N values. The nitrogen isotopic method of dietary analysis probably can be used to estimate the relative use of legumes vs non-legumes or of aquatic vs terrestrial organisms as food sources for extant and fossil animals. However, the method probably will not be applicable in those modern ecosystems in which the use of chemical fertilizers has influenced the distribution of nitrogen isotopes in food sources. The isotopic method of dietary analysis was used to reconstruct changes in the diet of the human population that occupied the Tehuacan Valley of Mexico over a 7000 yr span. Variations in the δ^(15)C and δ^(15)N values of bone collagen suggest that C_4 and/or CAM plants (presumably mostly corn) and legumes (presumably mostly beans) were introduced into the diet much earlier than suggested by conventional archaeological analysis.

5,548 citations

Journal ArticleDOI
TL;DR: The features of nanoparticle therapeutics that distinguish them from previous anticancer therapies are highlighted, and how these features provide the potential for therapeutic effects that are not achievable with other modalities are described.
Abstract: Nanoparticles — particles in the size range 1–100 nm — are emerging as a class of therapeutics for cancer. Early clinical results suggest that nanoparticle therapeutics can show enhanced efficacy, while simultaneously reducing side effects, owing to properties such as more targeted localization in tumours and active cellular uptake. Here, we highlight the features of nanoparticle therapeutics that distinguish them from previous anticancer therapies, and describe how these features provide the potential for therapeutic effects that are not achievable with other modalities. While large numbers of preclinical studies have been published, the emphasis here is placed on preclinical and clinical studies that are likely to affect clinical investigations and their implications for advancing the treatment of patients with cancer.

3,975 citations

Journal ArticleDOI
TL;DR: Lipidic nanoparticles are the first nanomedicine delivery system to make the transition from concept to clinical application, and they are now an established technology platform with considerable clinical acceptance.

3,497 citations

Journal ArticleDOI
TL;DR: Delivery of conventional chemotherapeutic anti-cancer drugs is mainly discussed and exploitation and the understanding of these characteristics to design new drug delivery systems targeting the tumor are focused on.

2,272 citations

Journal ArticleDOI
TL;DR: Stealth liposomes can be actively targeted with monoclonal antibodies or ligands and encapsulating active molecules, with high target efficiency and activity by synthetic modification of the terminal PEG molecule.
Abstract: Among several promising new drug-delivery systems, liposomes represent an advanced technology to deliver active molecules to the site of action, and at present several formulations are in clinical use. Research on liposome technology has progressed from conventional vesicles ("first-generation liposomes") to "second-generation liposomes", in which long-circulating liposomes are obtained by modulating the lipid composition, size, and charge of the vesicle. Liposomes with modified surfaces have also been developed using several molecules, such as glycolipids or sialic acid. A significant step in the development of long-circulating liposomes came with inclusion of the synthetic polymer poly-(ethylene glycol) (PEG) in liposome composition. The presence of PEG on the surface of the liposomal carrier has been shown to extend blood-circulation time while reducing mononuclear phagocyte system uptake (stealth liposomes). This technology has resulted in a large number of liposome formulations encapsulating active molecules, with high target efficiency and activity. Further, by synthetic modification of the terminal PEG molecule, stealth liposomes can be actively targeted with monoclonal antibodies or ligands. This review focuses on stealth technology and summarizes pre-clinical and clinical data relating to the principal liposome formulations; it also discusses emerging trends of this promising technology.

2,069 citations