B
Barry L. Wanner
Researcher at Harvard University
Publications - 131
Citations - 36349
Barry L. Wanner is an academic researcher from Harvard University. The author has contributed to research in topics: Gene & Escherichia coli. The author has an hindex of 63, co-authored 131 publications receiving 33446 citations. Previous affiliations of Barry L. Wanner include University of Michigan & Wayne State University.
Papers
More filters
Journal ArticleDOI
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
TL;DR: A simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s), which should be widely useful, especially in genome analysis of E. coli and other bacteria.
Journal ArticleDOI
Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection.
Tomoya Baba,Takeshi Ara,Miki Hasegawa,Yuki Takai,Yoshiko Okumura,Miki Baba,Kirill A. Datsenko,Masaru Tomita,Barry L. Wanner,Hirotada Mori,Hirotada Mori +10 more
TL;DR: These mutants—the ‘Keio collection’—provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome‐wide testing of mutational effects in a common strain background, E. coli K‐12 BW25113.
Journal ArticleDOI
Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence
Ekaterina Semenova,Matthijs M. Jore,Kirill A. Datsenko,Anna Semenova,Edze R. Westra,Barry L. Wanner,John van der Oost,Stan J. J. Brouns,Konstantin Severinov +8 more
TL;DR: It is proposed that the crRNA seed sequence plays a role in the initial scanning of invader DNA for a match, before base pairing of the full-length spacer occurs, which may enhance the protospacer locating efficiency of the E. coli Cascade complex.
Journal ArticleDOI
Escherichia coli K-12: a cooperatively developed annotation snapshot—2005
Monica Riley,Takashi Abe,Martha B. Arnaud,Mary B. Berlyn,Frederick R. Blattner,Roy R. Chaudhuri,Jeremy D. Glasner,Takashi Horiuchi,Ingrid M. Keseler,Takehide Kosuge,Hirotada Mori,Hirotada Mori,Nicole T. Perna,Guy Plunkett,Kenneth E. Rudd,Margrethe H. Serres,Gavin H. Thomas,Nicholas R. Thomson,David S. Wishart,Barry L. Wanner +19 more
TL;DR: A snapshot analysis based on the most recent genome sequences of two E.coli K-12 strains allows comparison of their genotypes and mutant status of alleles.
Journal ArticleDOI
Minimum information requested in the annotation of biochemical models (MIRIAM)
Nicolas Le Novère,Andrew Finney,Michael Hucka,Upinder S. Bhalla,Fabien Campagne,Julio Collado-Vides,Edmund J. Crampin,Matt D. B. Halstead,Edda Klipp,Pedro Mendes,Poul M. F. Nielsen,Herbert M. Sauro,Bruce E. Shapiro,Jacky L. Snoep,Hugh D. Spence,Barry L. Wanner +15 more
TL;DR: These rules define procedures for encoding and annotating models represented in machine-readable form to enable users to have confidence that curated models are an accurate reflection of their associated reference descriptions and to facilitate model reuse and composition into large subcellular models.