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Benjamin Harke
Researcher at Istituto Italiano di Tecnologia
Publications - 51
Citations - 3608
Benjamin Harke is an academic researcher from Istituto Italiano di Tecnologia. The author has contributed to research in topics: STED microscopy & Microscopy. The author has an hindex of 24, co-authored 51 publications receiving 3296 citations. Previous affiliations of Benjamin Harke include Max Planck Society.
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Journal ArticleDOI
STED microscopy with continuous wave beams.
TL;DR: In this article, the authors reported stimulated emission depletion (STED) fluorescence microscopy with continuous wave (CW) laser beams, achieving a resolution of 29-60 nm in the focal plane.
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Resolution scaling in STED microscopy
TL;DR: A comprehensive study of the inverse square root dependence of spatial resolution on the saturation factor in stimulated emission depletion (STED) microscopy is undertaken and the generalized square-root law is shown to predict the practical resolution with high accuracy.
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Anatomy and dynamics of a supramolecular membrane protein cluster
Jochen J. Sieber,Katrin I. Willig,Carsten Kutzner,Claas Gerding-Reimers,Benjamin Harke,Gerald Donnert,Burkhard Rammner,Christian Eggeling,Stefan W. Hell,Helmut Grubmüller,Thorsten Lang +10 more
TL;DR: A balance between self-association and crowding-induced steric repulsions is sufficient to explain both the size and dynamics of syntaxin clusters and likely of many oligomerizing membrane proteins that form supramolecular structures.
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Tuning of synapse number, structure and function in the cochlea
Alexander C. Meyer,Thomas Frank,Darina Khimich,Gerhard Hoch,Dietmar Riedel,Nikolai M. Chapochnikov,Yury M Yarin,Yury M Yarin,Benjamin Harke,Stefan W. Hell,Alexander Egner,Tobias Moser +11 more
TL;DR: In this article, the number of ribbon synapses per inner hair cell per IHC peaks where the cochlea is most sensitive to sound and showed substantial variability for presynaptic Ca(2+) signals even within individual IHCs.
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Bassoon and the Synaptic Ribbon Organize Ca2+ Channels and Vesicles to Add Release Sites and Promote Refilling
Thomas Frank,Thomas Frank,Mark A. Rutherford,Nicola Strenzke,Nicola Strenzke,Andreas Neef,Tina Pangršič,Darina Khimich,Anna Fejtova,Eckart D. Gundelfinger,M. Charles Liberman,Benjamin Harke,Keith E. Bryan,Amy S. Lee,Alexander Egner,Dietmar Riedel,Tobias Moser,Tobias Moser +17 more
TL;DR: Bassoon and the ribbon create a large number of release sites by organizing Ca²+ channels and vesicles, and promote vesicle replenishment in hair cell ribbon synapses following genetic disruption of the presynaptic scaffolds.