B
Bertrand Séraphin
Researcher at University of Strasbourg
Publications - 168
Citations - 25929
Bertrand Séraphin is an academic researcher from University of Strasbourg. The author has contributed to research in topics: RNA splicing & RNA. The author has an hindex of 72, co-authored 164 publications receiving 24966 citations. Previous affiliations of Bertrand Séraphin include University of Paris & French Institute of Health and Medical Research.
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Journal ArticleDOI
Functional organization of the yeast proteome by systematic analysis of protein complexes
Anne-Claude Gavin,Markus Bösche,Roland Krause,Paola Grandi,Martina Marzioch,Andreas Bauer,Jörg Schultz,Jens Rick,Anne-Marie Michon,Cristina-Maria Cruciat,Marita Remor,Christian Höfert,Malgorzata Schelder,Miro Brajenovic,Heinz Ruffner,Alejandro Merino,Karin Klein,Manuela Hudak,David Dickson,Tatjana Rudi,Volker Gnau,Angela Bauch,Sonja Bastuck,Bettina Huhse,Christina Leutwein,Marie-Anne Heurtier,Richard R. Copley,Angela Edelmann,Erich Querfurth,Vladimir Rybin,Gerard Drewes,Manfred Raida,Tewis Bouwmeester,Peer Bork,Bertrand Séraphin,Bernhard Kuster,Gitte Neubauer,Giulio Superti-Furga +37 more
TL;DR: The analysis provides an outline of the eukaryotic proteome as a network of protein complexes at a level of organization beyond binary interactions, which contains fundamental biological information and offers the context for a more reasoned and informed approach to drug discovery.
Journal ArticleDOI
A generic protein purification method for protein complex characterization and proteome exploration.
Guillaume Rigaut,Anna Shevchenko,Berthold Rutz,Matthias Wilm,Matthias Mann,Bertrand Séraphin +5 more
TL;DR: A generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag and Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein.
Journal ArticleDOI
The tandem affinity purification (TAP) method: a general procedure of protein complex purification.
Oscar Puig,Friederike Caspary,Guillaume Rigaut,Berthold Rutz,Emmanuelle Bouveret,Elisabeth Bragado-Nilsson,Matthias Wilm,Bertrand Séraphin +7 more
TL;DR: The TAP method is developed as a tool that allows rapid purification under native conditions of complexes, even when expressed at their natural level, and is a very useful procedure for protein purification and proteome exploration.
Journal ArticleDOI
Cryptic Pol II Transcripts Are Degraded by a Nuclear Quality Control Pathway Involving a New Poly(A) Polymerase
Françoise Wyers,Mathieu Rougemaille,Gwenael Badis,Jean-Claude Rousselle,Marie-Elisabeth Dufour,Jocelyne Boulay,Béatrice Regnault,Frédéric Devaux,Abdelkader Namane,Bertrand Séraphin,Bertrand Séraphin,Domenico Libri,Alain Jacquier +12 more
TL;DR: It is shown here that several supposedly silent intergenic regions in the genome of S. cerevisiae are actually transcribed by RNA polymerase II, suggesting that the expressed fraction of the genome is higher than anticipated.
Journal ArticleDOI
Positive feedback in eukaryotic gene networks: cell differentiation by graded to binary response conversion.
TL;DR: Positive feedback is used to construct a synthetic eukaryotic gene switch in Saccharomyces cerevisiae that resembles analog–digital conversion and has implications for understanding the graded and probabilistic mechanisms of enhancer action and cell differentiation.