B
Bethany Claridge
Researcher at La Trobe University
Publications - 10
Citations - 134
Bethany Claridge is an academic researcher from La Trobe University. The author has contributed to research in topics: Medicine & Internal medicine. The author has an hindex of 3, co-authored 5 publications receiving 29 citations. Previous affiliations of Bethany Claridge include Baker IDI Heart and Diabetes Institute.
Papers
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Journal ArticleDOI
Development of Extracellular Vesicle Therapeutics: Challenges, Considerations, and Opportunities.
Bethany Claridge,Bethany Claridge,Jonathan Lozano,Jonathan Lozano,Qi Hui Poh,Qi Hui Poh,David W. Greening +6 more
TL;DR: Extracellular vesicles (EVs) hold great promise as therapeutic modalities due to their endogenous characteristics, however, further bioengineering refinement is required to address clinical and commercial limitations as discussed by the authors.
Book ChapterDOI
A Protocol for Isolation, Purification, Characterization, and Functional Dissection of Exosomes.
Alin Rai,Haoyun Fang,Monique Fatmous,Monique Fatmous,Bethany Claridge,Bethany Claridge,Qi Hui Poh,Qi Hui Poh,Richard J. Simpson,David W. Greening,David W. Greening +10 more
TL;DR: In this article, a systematic "how to" protocol was proposed to obtain highly purified exosomes and perform their biophysical and biochemical characterization, which employs a mass spectrometry-based proteomics approach to characterize the protein composition of exosome.
Journal ArticleDOI
Proteomic dissection of large extracellular vesicle surfaceome unravels interactive surface platform.
Alin Rai,Alin Rai,Alin Rai,Haoyun Fang,Bethany Claridge,Bethany Claridge,Richard J. Simpson,David W. Greening +7 more
TL;DR: In this article, the authors used a membrane impermeant derivative of biotin to capture surface proteins coupled with mass spectrometry analysis, and showed that out of 4143 proteins identified in density-gradient purified L-EVs (1.07-1.11 g/mL, from multiple cancer cell lines), 961 proteins are surface accessible.
Journal ArticleDOI
Proteome characterisation of extracellular vesicles isolated from heart.
Bethany Claridge,Bethany Claridge,Alin Rai,Alin Rai,Alin Rai,Haoyun Fang,Aya Matsumoto,Jieting Luo,Julie R. McMullen,David W. Greening +9 more
TL;DR: In this article, the authors used Langendorff-collagenase-based enzymatic perfusion and differential centrifugation to isolate extracellular vesicles (cEVs) from mouse heart.
Journal ArticleDOI
Post-translational and transcriptional dynamics – regulating extracellular vesicle biology
TL;DR: This work has shown that protein post-translational modifications are emerging as major effectors of EV biology and function, and in turn, regulate cellular signaling, and further understanding of such modifications and intermolecular interactions will provide advances in EV-based therapeutic strategies.