Author
Biman B. Mandal
Other affiliations: Indian Institute of Technology Kharagpur, Indian Institutes of Technology, Tufts University
Bio: Biman B. Mandal is an academic researcher from Indian Institute of Technology Guwahati. The author has contributed to research in topics: Fibroin & Tissue engineering. The author has an hindex of 45, co-authored 153 publications receiving 6254 citations. Previous affiliations of Biman B. Mandal include Indian Institute of Technology Kharagpur & Indian Institutes of Technology.
Topics: Fibroin, Tissue engineering, SILK, Bombyx mori, Sericin
Papers
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TL;DR: This study indicates rapid freeze-drying technique as an alternative method to fabricate highly interconnected porous scaffolds for developing functional 3D silk fibroin matrices for potential tissue engineering, biomedical and biotechnological applications.
490 citations
TL;DR: Development of a high compressive strength polymeric bone composite materials is reported, based on silk protein-protein interfacial bonding, which favored human bone marrow-derived mesenchymal stem cell differentiation toward bone-like tissue in vitro based on biochemical and gene expression for bone markers.
Abstract: Biomaterials for bone tissue regeneration represent a major focus of orthopedic research. However, only a handful of polymeric biomaterials are utilized today because of their failure to address critical issues like compressive strength for load-bearing bone grafts. In this study development of a high compressive strength (~13 MPa hydrated state) polymeric bone composite materials is reported, based on silk protein-protein interfacial bonding. Micron-sized silk fibers (10–600 µm) obtained utilizing alkali hydrolysis were used as reinforcement in a compact fiber composite with tunable compressive strength, surface roughness, and porosity based on the fiber length included. A combination of surface roughness, porosity, and scaffold stiffness favored human bone marrow-derived mesenchymal stem cell differentiation toward bone-like tissue in vitro based on biochemical and gene expression for bone markers. Further, minimal in vivo immunomodulatory responses suggested compatibility of the fabricated silk-fiber-reinforced composite matrices for bone engineering applications.
346 citations
TL;DR: This review reviewed various design considerations for skin repair and impact of several smart technologies viz., in situ 3D printing, portable bioprinters, electrosprayers and in situ forming hydrogels that have significantly improved wound healing and skin therapeutics.
290 citations
TL;DR: Rheological properties along with swellability, degradation, sol fraction estimation, equilibrium water content and swelling kinetics were evaluated, and MTT assay showed biocompatibility and absence of deleterious effects of hydrogel on cell viability and functionality.
263 citations
TL;DR: This study demonstrates the versatility of silk fibroin as a composite scaffolding material for use in cartilage tissue repair to create functional cartilage constructs that overcome the limitations of agarose biomaterials, and provide a much-needed alternative to the agarOSE standard.
205 citations
Cited by
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TL;DR: The ability of pore size and porosity of scaffolds to direct cellular responses and alter the mechanical properties of scaffold will be reviewed, followed by a look at nature's own scaffold, the extracellular matrix.
Abstract: Tissue engineering applications commonly encompass the use of three-dimensional (3D) scaffolds to provide a suitable microenvironment for the incorporation of cells or growth factors to regenerate ...
2,075 citations
01 Jan 2016
TL;DR: Fibroblasts of high population doubling level propagated in vitro, which have left the cell cycle, can carry out the contraction at least as efficiently as cycling cells as discussed by the authors, and the potential uses of the system as an immu- nologically tolerated "tissue" for wound hea ing and as a model for studying fibroblast function are discussed.
Abstract: Fibroblasts can condense a hydrated collagen lattice to a tissue-like structure 1/28th the area of the starting gel in 24 hr. The rate of the process can be regulated by varying the protein content of the lattice, the cell number, or the con- centration of an inhibitor such as Colcemid. Fibroblasts of high population doubling level propagated in vitro, which have left the cell cycle, can carry out the contraction at least as efficiently as cycling cells. The potential uses of the system as an immu- nologically tolerated "tissue" for wound hea ing and as a model for studying fibroblast function are discussed.
1,837 citations
TL;DR: The most relevant biopolymer-based hydrogel systems, the different methods of preparation, as well as an in depth overview of the applications in the field of tissue engineering will be given.
1,426 citations
01 Jan 2007
TL;DR: The terms "antioxidant", "oxidative stress" and "oxoidative damage" are widely used but rarely defined as discussed by the authors, and a brief review attempts to define them and to examine the ways in which oxidative stress and oxidative damage can affect cell behaviour both in vivo and in cell culture, using cancer as an example.
Abstract: The terms 'antioxidant', 'oxidative stress' and 'oxidative damage' are widely used but rarely defined. This brief review attempts to define them and to examine the ways in which oxidative stress and oxidative damage can affect cell behaviour both in vivo and in cell culture, using cancer as an example.
1,309 citations