Bin E. Li

TL;DR: This work identifies ENL as an unrecognized gene that is specifically required for proliferation in vitro and in vivo and suggests a mechanistic rationale for disrupting the YEATS domain in disease.

TL;DR: The CRISPR array repair lineage tracing (CARLIN) mouse line and corresponding analysis tools that can be used to simultaneously interrogate the lineage and transcriptomic information of single cells in vivo are described.

TL;DR: It is reported that EZH2, the catalytic subunit of Polycomb Repressive Complex 2 (PRC2), is overexpressed in CML LICs and required for colony formation and survival and cell-cycle progression of CML cell lines, and defines EZh2 as a selective vulnerability for CML cells and their LICs, regardless of BCR-ABL1 mutational status.

TL;DR: The CARLIN mouse line and corresponding analysis tools are described that can be used to simultaneously interrogate the lineage and transcriptomic information of single cells in vivo and allow the unbiased identification of transcriptional signatures based on in vivo stem cell function without a need for markers or cell sorting.

TL;DR: It is concluded that the progressively increased enhancer dependence of cell-type–specific genes with developmental age is conserved during development, characterized by simpler promoter-centric regulation of cell–type-specific genes in embryonic cells and increased combinatorial enhancer-driven control in adult cells.