Showing papers by "Bruce W. Birren published in 1992"
TL;DR: It is found that the clones based on Fosmid vector undergo detectable changes at a greatly reduced frequency and sequences that undergo drastic rearrangements and deletions during propagation in a conventional vector were stably propagated when recloned as Fosmids.
Abstract: Instability of complex mammalian genomic DNA inserts is commonplace in cosmid libraries constructed in conventional multicopy vectors. To develop a means to construct stable libraries, we have developed a low copy number cosmid vector based on the E.coli F factor replicon (Fosmid). We have tested relative stability of human DNA inserts in Fosmlds and in two conventional multicopy vectors (Lawrist 16 and Supercos) by comparing the frequency of changes In restriction patterns of the inserts after propagating randomly picked human genomic clones based on these vectors. We found that the clones based on Fosmid vector undergo detectable changes at a greatly reduced frequency. We also observed that sequences that undergo drastic rearrangements and deletions during propagation In a conventional vector were stably propagated when recloned as Fosmids. The results indicate that Fosmid system may be useful for constructing stable libraries from complex genomes.
278 citations
Patent•
26 Jun 1992
TL;DR: In this paper, a system for the separation of macromolecules in ADN is described. But the separation is performed by electrophorese. And the separation process is performed using electrophoresis.
Abstract: L'invention concerne un systeme destine a ameliorer la separation par electrophorese de macromolecules Elle concerne un systeme (100) qui comporte une base de connaissances (120) tiree de donnees d'etalonnage obtenues par separation de macromolecules, en particulier d'ADN, de taille connue, dans diverses conditions choisies pour l'optimisation des separations L'utilisateur saisit la plus petite taille et la plus grande taille de macromolecules d'ADN a separer D'apres cette information, le systeme (100) determine automatiquement les parametres de l'electrophorese de maniere qu'il ameliore sa resolution Application aux separations de macromolecules par electrophorese