Author
C. B. Barrett
Bio: C. B. Barrett is an academic researcher. The author has contributed to research in topics: Thin-layer chromatography & Silver nitrate. The author has an hindex of 1, co-authored 1 publications receiving 174 citations.
Topics: Thin-layer chromatography, Silver nitrate
Papers
More filters
TL;DR: In this paper, a procedure involving thin layer chromatography on silica impregnated with silver nitrate is described, which makes possible the separation of synthetic and natural glyceride mixtures into classes according to their degree of unsaturation, and within those classes the resolution of certain isomeric unsaturated glycerides.
Abstract: The paper describes a procedure involving thin layer chromatography on silica impregnated with silver nitrate, which makes possible the separation of synthetic and natural glyceride mixtures into classes according to their degree of unsaturation, and within those classes the resolution of certain isomeric unsaturated glycerides. The separations depend upon the ability of compounds having an olefinic double bond to form co-ordination complexes with the silver ion.
175 citations
Cited by
More filters
544 citations
TL;DR: The possibility of separating lipid materials on the basis of the number, type, and position of the unsaturated centers they contain, by virtue of the complexing of these unsaturated bonds with silver ions, provides a relatively recent but now very important addition to the range of separatory methods available to lipid chemists and biochemists.
398 citations
TL;DR: In this article, a method for the analysis of isomeric monoglycerides by thin-layer adsorption chromatography was developed for the separation of 1-and 2-monoglyceride.
Abstract: A method has been developed for the analysis of isomeric monoglycerides by thin-layer adsorption chromatography Isomeric monoglycerides are separated on silica gel impregnated with boric acid; a mixed solvent system is used The interaction of hydroxy compounds with boric acid allows the separation of 1- and 2-monoglycerides which cannot be resolved on silica gel alone In order to make the method quantitative, the resolved components are charred and their spot densities measured Glycerol, fatty acids, diglycerides, and triglycerides do not interfere The method does not induce isomerization
283 citations
TL;DR: A procedure for photodensitometric quantitation of thin-layer chromatograms is described which can give a complete analysis of a mixture of neutral lipids in a single chromatogram without the necessity for reference mixtures.
270 citations
TL;DR: The mechanism of silver ion chromatography is discussed in relation to recent studies using silver ion high-performance liquid chromatographic methodology, and those according to number, geometry and position of double bonds are discussed.
201 citations