Author
C. D. Nickell
Bio: C. D. Nickell is an academic researcher from University of Illinois at Urbana–Champaign. The author has contributed to research in topics: Callus & Phialophora gregata. The author has an hindex of 10, co-authored 11 publications receiving 1609 citations.
Papers
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1,309 citations
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TL;DR: Since the differences were not large, the results indicate that the tissue culture process is not necessarily detrimental to plant performance, which is an important consideration since tissue culture techniques are used in many genetic engineering methods.
Abstract: Genetic alterations of regenerated plants based on the tissue culture process (somaclonal variation) have become common for many plant species including soybean [Glycine max (L.) Merr.]. The objective of this study was to test for the presence of tissue-culture-derived genetic variation in eight agronomic traits in homozygous progeny regenerated by organogenesis using the commercially important cultivar Asgrow ‘A3127.’ A total of 86 lines derived by repeated self-pollination of nine regenerated plants was grown in two locations for 2 years. When compared to the unregenerated parent, statistically significant variation (P<0.05) was found for maturity, lodging, height, seed protein and oil, but not for seed quality, seed weight, or seed yield. All of the variation noted was beneficial and did not involve decreased yield. Since the differences were not large, the results indicate that the tissue culture process is not necessarily detrimental to plant performance, which is an important consideration since tissue culture techniques are used in many genetic engineering methods.
131 citations
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TL;DR: A highly significant correlation (r = 0.55) was observed between maturity and the genome size of the 20 cultivars and this relationship exists in soybean.
Abstract: Previous studies have reported a correlation between genome size and relative maturity among plant species The objective of this study was to determine whether such a relationship exists in soybean Twenty cultivars, representing maturity groups ranging from 000 to IX, were analyzed using flow cytometric procedures A 15% difference in genome size was observed ranging from ‘BSR 201’ at 288 pg to ‘Maple Presto’ at 251 pg A highly significant correlation (r = 055) was observed between maturity and the genome size of the 20 cultivars
63 citations
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TL;DR: It was indicated that F 2 phenotypic differences were largely due to environmental variation, thereby making yield selection for tolerance to Mn deficiency in the F 2 generation difficult, and selection based on F 2:3 family means should be effective.
Abstract: Manganese deficiency is a recurring problem in soybean [Glycine max (L.) Merr.] grown on high pH soils. Presently, the application of chemical fertilizers is used to alleviate yield losses associated with this deficiency. Because chemical control is often ineffective, genetic tolerance could be used in areas where Mn deficiency is a problem. The development of soybean genotypes tolerant to Mn deficiency is facilitated if the mode of inheritance is understood. The F 2 and F 2:3 progeny of the tolerant germplasm line A14 and the intolerant cultivar Pride B216 were evaluated in the field for tolerance to Mn deficiency. The observed wide range in progeny response to Mn deficiency suggested digenic inheritance. This model was supported by the results of a cluster analysis applied to the F 2:3 family mean ratings and variances. Low estimates of narrow sense heritability (k 2 = 0.19) in the F 2 generation indicated that F 2 phenotypic differences were largely due to environmental variation, thereby making yield selection for tolerance to Mn deficiency in the F 2 generation difficult. Estimates of narrow sense heritability based on F 2:3 family means were greater (h 2 = 0.70), indicating that selection based on F 2:3 family means should be effective.
20 citations
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TL;DR: The results show that in vitro evaluation for Fe efficiency can be a useful tool for plant breeders.
Abstract: The objectives of this study were: (i) to develop a tissue culture technique for the evaluation of Fe efficiency in soybean, and (ii) to compare the laboratory technique with field Fe chlorosis scores. Nineteen genotypes that had low and high levels of Fe efficiency were evaluated in the laboratory and at five field locations. Friable callus was induced from epicotyl sections, weighed, and placed on two different modified Murashige and Skoog media; one low in α-naphthaleneacetic acid and the other low in Fe. Callus growth was rated as lack of growth compared to respective controls. As an example, Fe-inefficient cultivars (‘Asgrow A3205’ and ‘Pride B216’) had significantly reduced growth compared to Fe-efficient germ plasm lines (‘All’ and ‘A14’). Correlation between the laboratory and field chlorosis rating was highest for the low auxin medium (r2 = 0.78), although correlation for the low Fe medium was also significant (r2 = 0.72). These results show that in vitro evaluation for Fe efficiency can be a useful tool for plant breeders.
20 citations
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12 Feb 2004
TL;DR: A novel soybean cultivar, designated 0509244, is disclosed in this article, which relates to the seeds of soybean and plants of the soybean 0509 244 and to methods for producing a soybean plant produced by crossing the cultivar with itself or another soybean variety.
Abstract: A novel soybean cultivar, designated 0509244, is disclosed. The invention relates to the seeds of soybean cultivar 0509244, to the plants of soybean 0509244 and to methods for producing a soybean plant produced by crossing the cultivar 0509244 with itself or another soybean variety. The invention further relates to hybrid soybean seeds and plants produced by crossing the cultivar 0509244 with another soybean cultivar.
1,165 citations
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12 Feb 2004
TL;DR: In this paper, a novel soybean cultivar, designated 0509240, was proposed and a method for producing a soybean plant produced by crossing the cultivar with itself or another soybean variety.
Abstract: A novel soybean cultivar, designated 0509240, is disclosed. The invention relates to the seeds of soybean cultivar 0509240, to the plants of soybean 0509240 and to methods for producing a soybean plant produced by crossing the cultivar 0509240 with itself or another soybean variety. The invention further relates to hybrid soybean seeds and plants produced by crossing the cultivar 0509240 with another soybean cultivar.
1,148 citations
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TL;DR: Current procedures for estimation of absolute DNA amounts in plants using flow cytometry are reviewed, with special emphasis on preparation of nuclei suspensions, stoichiometric DNA staining and the use of DNA reference standards.
844 citations
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TL;DR: A brief review of studies in the area of phytoaccumulation is provided, most of which have been carried out in Europe and the USA, with particular attention given to the role of phytochelators in making the heavy metals bio- available to the plant and their symbionts in enhancing the uptake of bio-available heavy metals.
806 citations
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TL;DR: The results obtained in this study demonstrate that flow cytometry with DNA intercalators is a reliable method for estimation of nuclear genome size in plants, and confirmed an urgent need for an agreement on standards.
583 citations