We have used the Escherichia coli beta-glucuronidase gene (GUS) as a gene fusion marker for analysis of gene expression in transformed plants. Higher plants tested lack intrinsic beta-glucuronidase activity, thus enhancing the sensitivity with which measurements can be made. We have constructed gene fusions using the cauliflower mosaic virus (CaMV) 35S promoter or the promoter from a gene encoding the small subunit of ribulose bisphosphate carboxylase (rbcS) to direct the expression of beta-glucuronidase in transformed plants. Expression of GUS can be measured accurately using fluorometric assays of very small amounts of transformed plant tissue. Plants expressing GUS are normal, healthy and fertile. GUS is very stable, and tissue extracts continue to show high levels of GUS activity after prolonged storage. Histochemical analysis has been used to demonstrate the localization of gene activity in cells and tissues of transformed plants.

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GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

Keywords: Time-Resolved Fluorescence ; Resonance Energy-Transfer ; Near-Infrared Luminescence ; Double-Stranded Dna ; Prostate-Specific Antigen ; Photoinduced Electron-Transfer ; Europium-Tetracycline Complex ; Sybr-Green-I ; Terbium Complexes ; Optical Probes Reference EPFL-ARTICLE-149396doi:10.1021/cr900362eView record in Web of Science Record created on 2010-06-17, modified on 2017-05-12

Lanthanide Luminescence for Biomedical Analyses and Imaging

The developmental types of secretory cavities in Citrus remain unclear and whether or not programmed cell death is involved in the developmental process of secretory cavities remains an enigma. Regarding cavity formation in Citrus sinensis fruits, this work uncovered novel evidence to delineate secretory cavity formation in schizolysigeny, supporting the possibility of utilizing secretory cavities as a new cell biology model for investigating the molecular mechanism of plant programmed cell death.

Apoptosis. The role of the endonuclease

Fluorometric assay of DNA in cartilage explants using Hoechst 33258.

https://www.ucl.ac.uk/%7Eucbzwdr/publications/Barres%20et%20al%20%28Cell%201992%29.pdf

Cell death and control of cell survival in the oligodendrocyte lineage

A simple, rapid, and sensitive DNA assay procedure.

Publisher Summary Glucuronidase is useful in dividing mutations into four categories: structural genes, regulatory genes, processing genes, and temporal genes. This chapter discusses regulatory genes that affect the synthesis of mouse kidney glucuronidase in response to androgens. A kinetic analysis of induction suggests that these properties would be accounted for if this gene determines the affinity of the chromosomal region for the androgen-receptor protein. Genetic analysis of these mutants should determine whether much regulatory information is present around the enzyme structural gene in mammals. The pearl mutation is a strikingly different type of regulatory mutant for it apparently increases glucuronidase synthesis by sending signals from a chromosomal site far removed from the glucuronidase structural gene. Further, it increases the activity of several lysosomal enzymes. The mutants are of interest in that they may affect the functions of several kinds of subcellular organelles. The beige mutation, for example, causes altered morphology or function of histamine storage granules, platelet storage granules, and lamellar bodies of alveolar cells. Although their gene products are still unknown, the pigment mutants should be useful in defining the biogenesis, processing, functions, and interrelationships of the subcellular organelles.

Genetic regulation of mammalian glucuronidase.

A regulatory locus in a higher organism has been shown to control a specific messenger RNA activity. The Gur locus in mice regulates the production of kidney beta-glucuronidase messenger RNA activity after induction of the beta-glucuronidase structural gene, Gus, by testosterone. beta-Glucuronidase messenger RNA was assayed by its ability to direct the synthesis of catalytically active murine beta-glucuronidase in Xenopus oocytes.

C Labarca

Papers

A simple, rapid, and sensitive DNA assay procedure.

Genetic regulation of mammalian glucuronidase.

A regulatory locus for mouse beta-glucuronidase induction, Gur, controls messenger RNA activity

Genetic determination of kinetic parameters in beta-glucuronidase induction by androgen.