C. M. Wilson

Bio: C. M. Wilson is an academic researcher. The author has contributed to research in topics: Quantitative analysis (chemistry). The author has an hindex of 1, co-authored 1 publications receiving 216 citations.

On the Chemical Composition of Eleven Species of Marine Phytoplankters

Abstract: Eleven species of marine planktonic representatives of the Chlorophyceae, Chrysophyceae, Bacillariophyceae, Dinophyceae and Myxophyceae have been analyzed for their chemical composition. All specie...

The Protein Component of Primary Cell Walls

Abstract: Publisher Summary The purpose of this chapter is to present data and discusses cell-wall protein from several points of view ranging from the purely enzymic aspects (cell-wall enzymes) to the structural hydroxyproline-rich wall proteins. Although the subject of wall protein is hardly a new field it does begin to look as though the demonstration of wall-bound hydroxyproline has imparted a revivifying stimulus to an old problem-this is more evident from work known to be in progress than from the amount of published material. Physically and chemically, the anisotropic cambial walls contain both cellulose and polyuronides. Electron microscopic studies show that more or less randomly interwoven cellulose microfibrils are recognized as another definitive characteristic of primary cell walls. Isolation of a clean wall fraction from various parts of whole plants is much more difficult than from suspension cultures. An important point is that the primary wall is often contaminated with secondary wall. This increases the difficulty of cell breakage and removal of the cell contents.

Biochemical Studies on the Thermal Dimorphism of Paracoccidioides brasiliensis

Abstract: The biochemical and morphological changes of the yeastlike (Y) form to the mycelial (M) form of Paracoccidioides brasiliensis were examined. The main polysaccharide of hexoses of the Y-form cell wall was alpha-glucan, whereas the polysaccharides of the M-form cell wall were beta-glucan and galactomannan. The alpha-glucan of the Y form contained mainly alpha-(1 --> 3)-glycosidic linkage. The beta-glucan of the M form contained mainly beta-(1 --> 3)-glycosidic linkage with a few branches at C-6 position. The incorporation of (14)C-glucose into the cell wall glucans showed that synthesis of alpha-glucan decreased rapidly after the temperature of the culture was changed from 37 to 20 C. The synthesis of beta-glucan was augmented at an early stage of the morphological change. The M-form cell wall contained 12 times more disulfide linkage than the Y form. The cell-free extracts of the whole cell of the Y form had five times more protein disulfide reductase activity than the M form, whereas extracts of the M form contained five to eight times more beta-glucanase activity than the Y form. From these results, a hypothesis for the production of the M form from the Y form is proposed.