Microfluidic paper-based platform for whole blood creatinine detection

Biosensing methods for determination of creatinine: A review

This review (with 340 refs) focuses on methods for specific and sensitive detection of metabolites for diagnostic purposes, with particular emphasis on electrochemical nanomaterial-based sensors. It also covers novel candidate metabolites as potential biomarkers for diseases such as neurodegenerative diseases, autism spectrum disorder and hepatitis. Following an introduction into the field of metabolic biomarkers, a first major section classifies electrochemical biosensors according to the bioreceptor type (enzymatic, immuno, apta and peptide based sensors). A next section covers applications of nanomaterials in electrochemical biosensing (with subsections on the classification of nanomaterials, electrochemical approaches for signal generation and amplification using nanomaterials, and on nanomaterials as tags). A next large sections treats candidate metabolic biomarkers for diagnosis of diseases (in the context with metabolomics), with subsections on biomarkers for neurodegenerative diseases, autism spectrum disorder and hepatitis. The Conclusion addresses current challenges and future perspectives.

Advances in the design of nanomaterial-based electrochemical affinity and enzymatic biosensors for metabolic biomarkers: A review.

Modern creatinine (Bio)sensing: Challenges of point-of-care platforms.

In this article, a novel and efficient electrochemical sensor of creatinine was facilely constructed on the surface of multifunctional nanohybrids-modified glassy carbon electrode (GCE), integrating creatinine-induced specific signal response and enzymeless ratiometric signal detection strategy. Dopamine hydrochloride as a reducing agent was dispersed in interface layers of graphene oxide. One-step hydrothermal reaction of graphene oxide with dopamine hydrochloride was conducted to prepare reduced graphene oxide (rGO), together with self-polymerization of polydopamine (PDA) attaching on rGO. Nile blue (NB) was loaded on rGO under ultrasonication through π-π stacking interactions between rGO and NB. The resulting PDA-rGO-NB complex was drop-coated on the surface of GCE. Under cyclic voltammetry scanning, copper nanoparticles (CuNPs) were prepared through electrochemical reduction of Cu2+ ions and electrodeposited on PDA-rGO-NB surface to construct a new CuNPs/PDA-rGO-NB/GCE sensing platform. Specific interactions of creatinine with Cu2+ ions from CuNPs surface led to regularly decreased peak current intensities located at –0.05 V (ICu2+) and negligible changes in peak current intensities located –0.325 V from NB (INB). There was a well plotted linear relationship between ICu2+/INB and creatinine concentration in the range from 0.01 to 100 μM, with a low detection limit of 2 nM. This sensing platform enabled highly sensitive and selective responses towards creatinine, over potential interferents. In practical samples, this developed enzymeless ratiometric electrochemical sensor realized efficient detection of creatinine, showing high detection performance.

Creatinine-induced specific signal responses and enzymeless ratiometric electrochemical detection based on copper nanoparticles electrodeposited on reduced graphene oxide-based hybrids

C.S. Pundir

Papers

An improved amperometric creatinine biosensor based on nanoparticles of creatininase, creatinase and sarcosine oxidase