Camila Hatsu Pereira Yoshida

Bio: Camila Hatsu Pereira Yoshida is an academic researcher from University of Western Ontario. The author has contributed to research in topics: Chemistry & Shoot. The author has an hindex of 3, co-authored 7 publications receiving 21 citations.

Response of soybean to soil waterlogging associated with iron excess in the reproductive stage.

Abstract: Soil waterlogging is a common problem in some agricultural areas, including regions under soybean (Glycine max) cultivation. In waterlogged soils, soil O2 depletion occurs due to aerobic microorganisms and plants, affecting the metabolic and physiological processes of plants after suffering anoxia in their root tissue. Another harmful factor in this situation is the exponential increase in the availability of iron (Fe) in the soil, which may result in absorption of excess Fe. The present study sought to evaluate the response mechanisms in soybean leaves ‘Agroeste 3680’ by physiological and biochemical analyses associating them with the development of pods in non-waterlogged and waterlogged soil, combined with one moderate and two toxic levels of Fe. Gas exchange was strongly affected by soil waterlogging. Excess Fe without soil waterlogging reduced photosynthetic pigments, and potentiated this reduction when associated with soil waterlogging. Starch and ureide accumulation in the first newly expanded trifoliate leaves proved to be response mechanisms induced by soil waterlogging and excess Fe, since plants cultivated under soil non-waterlogged soil at 25 mg dm−3 Fe showed lower contents when compared to stressed plants. Thus, starch and ureide accumulation could be considered efficient biomarkers of phytotoxicity caused by soil waterlogging and excess Fe in soybean plants. The reproductive development was abruptly interrupted by the imposition of stresses, leading to a loss of pod dry biomass, which was largely due to the substantial decrease in the net photosynthetic rate, as expressed by area (A), the blockage of carbohydrate transport to sink tissues and an increase of malondialdehyde (MDA). The negative effect on reproductive development was more pronounced under waterlogged soil.

Methyl jasmonate modulation reduces photosynthesis and induces synthesis of phenolic compounds in sweet potatoes subjected to drought

Abstract: Sweet potato [Ipomoea batatas (L.) Lam.] has wide adaptability to different climatic conditions. However, its yield can be affected by prolonged periods of drought. Application of exogenous jasmonates can modulate several physiological and biochemical processes, improving plant tolerance to abiotic stress. This study sought to evaluate the role of exogenous application of methyl jasmonate (MeJA) in attenuating the adverse effects of drought stress by physiobiochemical analyses and their impact during the early initiation of tuberous roots. The experimental design was completely randomized and arranged in a 2 x 2 factorial, comprised of two concentrations of a MeJA plant regulator [without (0 µmol·L-1) and with (13 µmol·L-1) application] and two water regimes (optimum and drought conditions, corresponding to a field capacity of 100 and 40%, respectively). Plants treated with MeJA showed a reduction in total leaf area and leaf dry biomass but increased adventitious root dry biomass. In addition, MeJA application in sweet potato plants affected photosynthetic performance and increased and antioxidant phenolic compounds, carotenoids, anthocyanins, and proline. The evaluated response mechanisms showed that the severity of drought was more prominent than the positive effects of MeJA, since the increases on antioxidant pigments and secondary metabolites were not sufficient to mitigate stress caused by drought, which was reflected in the reduced tuberous root production.

Physiological and toxic effects of selenium on seed germination of cowpea seedlings

Abstract: Selenium (Se) is considered a beneficial chemical element for plants, but in high concentrations it may present symptoms of toxicity. The present study aimed to evaluate 11 concentrations of Se (0; 0.1; 0.5; 1; 5; 10; 20; 40; 80; 400; 800 mg.L-1) to determine the low and high (toxicity) critical levels to seed germination of cowpea (Vigna unguiculata). In addition, alterations in the rate of photosynthetic pigments, lipid peroxidation and sugars during the initial growth development of seedlings were analysed. Seeds exposed to 800 mg.L-1 of Se showed a decrease of 20% of seed germination index compared to the control treatment. The decrease in seedling growth reflected in the increase of total sugars and sucrose concentration in both the shoot and root in response to exposure to Se concentration. There was a decrease in the concentration of leaf chlorophyll, carotenoids and pheophytin from seedlings exposed to high Se concentration. The rate of lipid peroxidation and the hydrogen peroxide concentration in the shoot was reduced up to the concentration of 1 mg.L-1 with subsequent increase in response to Se concentration applied. In the roots, the lipid peroxidation rate increased at concentrations higher than 80 mg.L-1. The highest oxidation rate of the cellular lipid membrane in response to Se occurred in the shoot, due to oxidation reactions in the chloroplast. Degradation of photosynthetic pigments and accumulation of total sugars and sucrose can be considered efficient biomarkers to indicate the toxicity of Se in cowpea seedlings and probably in other crops.

Iron toxicity: effects on the plants and detoxification strategies

Abstract: Iron (Fe) is an essential micronutrient for plants, as a cofactor in multi-heme cytochromes and within iron–sulfur clusters. However, Fe can be toxic at high concentrations. Free Fe in cells can disrupt the cell redox balance toward a pro-oxidant state, generating oxidative stress. The focuses of this review were to elucidate the Fe detoxification strategies used by plants, as well as describe the Fe excess effects on the plant body and its impact on the physiological, morphological and metabolic traits. Therefore, we highlight the importance of evaluating Fe toxicity and provide a paper compilation on Fe detoxification strategies and morpho-physiological responses to excess Fe, directing further research in this segment.

Application of 2,4-D hormetic dose associated with the supply of nitrogen and nickel on cotton plants.

Abstract: Growth traits, yield, N content, photosynthetic pigments, ammonia and amino acids were measured to verify the effect of the interaction between N, Ni, and 2,4-D applied in the cotton crop. The objective was to study the hormonal effect of 2,4-D associated with the application of N and Ni in coverage to improve yield. The N (0, 40, 80, and 120 kg ha-1) and Ni(0, 300, 450, and 600 g ha-1) were applied to the soil in the square phenological growth stage. The commercial 2,4-D DMA® BR (0 and 1.8 g a.e ha-1) was applied to the leaves at the same growth stage. The supply of N in cover fertilization up to 120 kg ha-1 was beneficial for cotton, providing greater yield and content of photosynthetic pigments. The application of 2,4-D in a hormetic dose, as a synthetic auxin during the beginning of flowering, proved to be a promising technique to improve cotton yield. This end-of-cycle response is related to the requirement for auxins during the cotton fruiting process.

The Role of Plant Growth-Promoting Bacteria in Alleviating the Adverse Effects of Drought on Plants

Abstract: Plant growth-promoting bacteria play an essential role in enhancing the physical, chemical and biological characters of soils by facilitating nutrient uptake and water flow, especially under abiotic stress conditions, which are major constrains to agricultural development and production. Drought is one of the most harmful abiotic stress and perhaps the most severe problem facing agricultural sustainability, leading to a severe shortage in crop productivity. Drought affects plant growth by causing hormonal and membrane stability perturbations, nutrient imbalance and physiological disorders. Furthermore, drought causes a remarkable decrease in leaf numbers, relative water content, sugar yield, root yield, chlorophyll a and b and ascorbic acid concentrations. However, the concentrations of total phenolic compounds, electrolyte leakage, lipid peroxidation, amounts of proline, and reactive oxygen species are considerably increased because of drought stress. This negative impact of drought can be eliminated by using plant growth-promoting bacteria (PGPB). Under drought conditions, application of PGPB can improve plant growth by adjusting hormonal balance, maintaining nutrient status and producing plant growth regulators. This role of PGPB positively affects physiological and biochemical characteristics, resulting in increased leaf numbers, sugar yield, relative water content, amounts of photosynthetic pigments and ascorbic acid. Conversely, lipid peroxidation, electrolyte leakage and amounts of proline, total phenolic compounds and reactive oxygen species are decreased under drought in the presence of PGPB. The current review gives an overview on the impact of drought on plants and the pivotal role of PGPB in mitigating the negative effects of drought by enhancing antioxidant defense systems and increasing plant growth and yield to improve sustainable agriculture.