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Carl L. Hansen
Researcher at University of British Columbia
Publications - 100
Citations - 10210
Carl L. Hansen is an academic researcher from University of British Columbia. The author has contributed to research in topics: Stem cell & Protein crystallization. The author has an hindex of 43, co-authored 100 publications receiving 8904 citations. Previous affiliations of Carl L. Hansen include Institute for Systems Biology & California Institute of Technology.
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Journal ArticleDOI
Long-Term Monitoring of Bacteria Undergoing Programmed Population Control in a Microchemostat
TL;DR: The microfluidic bioreactor enabled long-term monitoring of unnatural behavior programmed by the synthetic circuit, which included sustained oscillations in cell density and associated morphological changes, over hundreds of hours.
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A robust and scalable microfluidic metering method that allows protein crystal growth by free interface diffusion.
TL;DR: This work developed a scheme for metering fluids on the picoliter scale that is scalable to highly integrated parallel architectures and is independent of the properties of the working fluid, and demonstrates that diffraction-quality crystals may be grown and harvested from such nanoliter-volume reactions.
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Dynamics of genomic clones in breast cancer patient xenografts at single-cell resolution
Peter Eirew,Peter Eirew,Adi Steif,Adi Steif,Jaswinder Khattra,Jaswinder Khattra,Gavin Ha,Gavin Ha,Damian Yap,Damian Yap,Hossein Farahani,Hossein Farahani,Karen A. Gelmon,Stephen Chia,Colin Mar,Adrian Wan,Emma Laks,Emma Laks,Justina Biele,Justina Biele,Karey Shumansky,Jamie Rosner,Andrew McPherson,Andrew McPherson,Cydney B. Nielsen,Cydney B. Nielsen,Andrew Roth,Andrew Roth,Calvin Lefebvre,Calvin Lefebvre,Ali Bashashati,Ali Bashashati,Camila P. E. de Souza,Celia Siu,Radhouane Aniba,Radhouane Aniba,Jazmine Brimhall,Arusha Oloumi,Arusha Oloumi,Tomo Osako,Tomo Osako,Alejandra Bruna,José Luis Sandoval,Teresa Ruiz de Algara,Teresa Ruiz de Algara,Wendy Greenwood,Kaston Leung,Hongwei Cheng,Hongwei Cheng,Hui Xue,Hui Xue,Yuzhuo Wang,Yuzhuo Wang,Dong Lin,Dong Lin,Andrew J. Mungall,Richard D. Moore,Yongjun Zhao,Julie Lorette,Long V. Nguyen,Long V. Nguyen,David G. Huntsman,David G. Huntsman,Connie J. Eaves,Connie J. Eaves,Carl L. Hansen,Marco A. Marra,Carlos Caldas,Sohrab P. Shah,Sohrab P. Shah,Samuel Aparicio +70 more
TL;DR: The results show that measurement of genomically defined clonal population dynamics will be highly informative for functional studies using patient-derived breast cancer xenoengraftment, and indicates that genomic aberrations can be reproducible determinants of evolutionary trajectories.
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Microfluidic Synthesis of Highly Potent Limit-size Lipid Nanoparticles for In Vivo Delivery of siRNA
Nathan M. Belliveau,Jens Huft,Paulo Jc Lin,Sam Chen,Alex K. K. Leung,Timothy Leaver,Andre Wild,Justin B. Lee,Robert Joseph Taylor,Ying K. Tam,Carl L. Hansen,Pieter R. Cullis +11 more
TL;DR: It is shown that microfluidic mixing techniques, which permit millisecond mixing at the nanoliter scale, can reproducibly generate limit size LNP siRNA systems 20 nm and larger with essentially complete encapsulation of siRNA over a wide range of conditions with polydispersity indexes as low as 0.02.
Journal ArticleDOI
High-throughput microfluidic single-cell RT-qPCR
Adam K. White,Michael VanInsberghe,Oleh Petriv,Mani Hamidi,Darek Sikorski,Marco A. Marra,James M. Piret,Samuel Aparicio,Carl L. Hansen +8 more
TL;DR: This work presents a fully integrated microfluidic device capable of performing high-precision RT-qPCR measurements of gene expression from hundreds of single cells per run, and shows that nanoliter volume processing reduced measurement noise, increased sensitivity, and provided single nucleotide specificity.