Carla Connelly

TL;DR: It is shown that previously known and new genes are necessary for optimal growth under six well-studied conditions: high salt, sorbitol, galactose, pH 8, minimal medium and nystatin treatment, and less than 7% of genes that exhibit a significant increase in messenger RNA expression are also required for optimal Growth in four of the tested conditions.

TL;DR: A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome), finding that 17 percent were essential for viability in rich medium.

TL;DR: A genetic system to recover many new L1 insertions in somatic cells was developed that faithfully mimic many aspects of L1s that accumulated since the primate radiation, and short identical sequences were shared between the donor and the target site's 3' end, suggesting a mechanistic model that helps explain the structure of L 1 insertions.

TL;DR: Characterization of these mutant strains will describe the functions of gene products crucial to the successful execution of processes required for aspects of the chromosome cycle that are important for chromosome transmission fidelity in mitosis.

TL;DR: Identification of Skp1p homologs from C. elegans, A. thaliana, and H. sapiens indicates that SKP1 is evolutionarily highly conserved, and represents an intrinsic kinetochore protein conserved throughout eukaryotic evolution and may be directly involved in linking kinETochore function with the cell cycle-regulatory machinery.