Showing papers by "Carlo M. Croce published in 1972"
TL;DR: Although there was a significant effect on the efficiency of cell fusion, the critical time for the pH effect was in the first 4-8 days after fusion, presumably while viable hybrids were being formed from the multinucleated heterokaryocytes.
Abstract: The hybridization of a human and mouse cell was strikingly pH-dependent, with a well defined optimum at (about) pH 7.6-8.0. The yield of hybrid cell colonies (1 per 500-2000 heterokaryocytes) was several hundred times greater than that obtained at pH 6.8-7.2. Although there was a significant effect on the efficiency of cell fusion, the critical time for the pH effect was in the first 4-8 days after fusion, presumably while viable hybrids were being formed from the multinucleated heterokaryocytes.
72 citations
TL;DR: Fusion of rabbit cauda epididymal or ejaculate spermatozoa with F5-1 Syrian hamster somatic cells in the presence of lysolecithin resulted in the formation of heterokaryocytes, initiated disaggregation of spermutozoal chromatin and stimulated DNA synthesis by the disaggregated chromatin.
42 citations