Showing papers by "Carlo M. Croce published in 1980"
TL;DR: A stem cell is constructed which contains a set of genes (SV40), the expression of which is regulated differently in stem and differentiated cells, which could be used to determine the mechanism of suppression of expression of these genes in stem cells.
Abstract: Thymidine kinase-deficient (TK-; ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21)F9 teratocarcinoma stem cells have been transformed with a recombinant plasmid genome consisting of the pBR322 genome linked to a herpes simplex virus type 1 thymidine kinase gene (HSV-1 tk) and a simian virus 40 (SV40) genome. A clonal line of stem cells was obtained that contains only one copy of plasmid DNA, which is integrated into murine chromosomal DNA through a site on the pBRR322 genome. The HSV-1 tk gene, which is adjacent to the SV40 genome, is expressed in stem cells, whereas SV40 gene expression is not detectable. If differentiation of these stem cells is induced, the differentiated cells express SV40 early gene products. Thus, we have constructed a stem cell which contains a set of genes (SV40), the expression of which is regulated differently in stem and differentiated cells. This cell line could be used to determine the mechanism of suppression of expression of these genes in stem cells.
55 citations
TL;DR: A long-lasting, but not permanent, increase in XO activity was observed in mice during bacterial or protozoal infection or with Ehrlich ascitic carcinoma, and a nonspecific defence mechanism is indicated, probably involving enhanced oxidative processes.
52 citations
TL;DR: Somatic cell hybrids between totipotent mouse teratocarcinoma and myeloma cells are produced and should make it possible to study the expression of already rearranged immunoglobulin genes during mouse development.
Abstract: We have produced somatic cell hybrids between totipotent mouse teratocarcinoma and myeloma cells. These hybrids behave like the teratocar-cinoma cell parent and express teratocarcinoma embryonal antigens. However, they also express the myeloma H-2 antigens. The availability of these hybrids should make it possible to study the expression of already rearranged immunoglobulin genes during mouse development.
13 citations