Carol A. Ziomek

TL;DR: The in-vitro development of 1-cell embryos beyond the 2-cell stage in response to the removal of glucose and the addition of glutamine to the culture medium suggests that glucose may block some essential metabolic process, and that glutamine may be a preferred energy substrate during early development for these mouse embryos.

TL;DR: Variations of CZB medium were tested for their ability to support the development of 1-cell embryos from 4 strains of mice and optimum development to morula and blastocyst stages was in C ZB medium containing both glutamine and glucose.

TL;DR: Phylogenetic analysis indicates that the three mouse AP isozymes form a distinct group separate from the human tissue-specific AP iszymes, suggesting the taxon-specific evolution of the AP genes as opposed to independent evolution of AP genes expressed in specific tissues.

TL;DR: A variety of cell types of known APase reactivity were stained by this protocol and F9 teratocarcinoma cells, intestinal epithelial cells, and rat fetal primordial germ cells were all found to be highly positive for APase activity, in agreement with published results on APase localization in these cells.

TL;DR: One‐cell mouse embryos that block at the 2‐cell stage can progress to the morula stage in CZB medium, but fail to cavitate and then swell and lyse, but a 1‐min exposure to 27 mM glucose at the 4‐ cell stage will support a high frequency of development to the blastocyst stage in the same medium.